scholarly journals Pseudomonas alcaligenes

2020 ◽  
Author(s):  
Keyword(s):  
Pseudomonas Alcaligenes

Adsorptive Removal of Cd(II) by a Novel Extracellular Biopolymer Produced by Pseudomonas alcaligenes: Equilibrium and Kinetics

2010 ◽  
Vol 171-172 ◽  
pp. 15-18
Author(s):  
Zeng Quan Ji ◽  
Tian Hai Wang ◽  
Kai Hong Luo ◽  
Yao Qing Wang
Keyword(s):  
Chemical Interaction ◽  
Kinetic Studies ◽  
Maximum Adsorption Capacity ◽  
Metallic Ions ◽  
Equilibrium Studies ◽  
Kinetic Rates ◽  
Equilibrium And Kinetics ◽  
Potential Applications ◽  
Pseudo Second Order ◽  
Pseudomonas Alcaligenes

An extracellular biopolymer (PFC02) produced by Pseudomonas alcaligenes was used as an alternative biosorbent to remove toxic Cd(II) metallic ions from aqueous solutions. The effect of experimental parameters such as pH, Cd(II) initial concentration and contact time on the adsorption was studied. It was found that pH played a major role in the adsorption process, the optimum pH for the removal of Cd(II) was 6.0. The FTIR spectra showed carboxyl, hydroxyl and amino groups of the PFC02 were involved in chemical interaction with the Cd(II) ions. Equilibrium studies showed that Cd(II) adsorption data followed Langmuir model. The maximum adsorption capacity (qmax) for Cd(II) ions was estimated to be 93.55 mg/g. The kinetic studies showed that the kinetic rates were best fitted to the pseudo-second-order model. The study suggestted that the novel extracellular biopolymer biosorbent have potential applications for removing Cd(II) from wastewater.

scholarly journals Lignolytic Enzyme Activity of Isolated Bacteria from Termite (Coptotermes Sp.) and Milkfish (Chanos chanos Forsskal, 1775) Guts

2021 ◽  
Vol 13 (1) ◽  
pp. 70-76
Author(s):  
Emi Latifah ◽  
Putri Dwi Mulyani ◽  
Yekti Asih Purwestri
Keyword(s):  
Enzyme Activity ◽  
Bacillus Licheniformis ◽  
Test Method ◽  
Chanos Chanos ◽  
Enzyme Isolation ◽  
Optimum Ph ◽  
Qualitative Test ◽  
Pseudomonas Alcaligenes ◽  
Enzyme Source ◽  
Brevibacillus Parabrevis

Bacteria BSR 2, Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), Brevibacillus sp. (BSR 9), isolated from termite gut and Bacillus licheniformis (BSA B1) isolated from milkfish gut have been known to possess celluloytic activity. However, their lignolytic ability has not been known. This study aimed to determine the lignolytic ability of bacteria isolated from termit (Coptotermes sp.) and milkfish (Chanos chanos Forsskal, 1775) guts and their enzymes characterization. The qualitative test was done through the spot test method, while quantitative assay was performed spectrophotometrically at 335 nm to calculate vanillin concentration. The isolates were grown in Lignin Mineral Medium, then the optical density (OD620) were measured every 24 hours for 5 days using spectrophotometer to determine their growth profile and the best isolation time of the lignolytic enzyme. Based on results, the best lignolytic enzyme isolation time for strains Bacillus licheniformis (BSA B1) and BSR 2 were 5 days, yielding lignolytic enzyme activity of 0.961 ± 0.168 U/mg and 2.176 ± 0.088 U/mg respectively,  while strains Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), and Brevibacillus sp. (BSR 9) were 4 days, yielding of 1.206 ± 0.045 U/mg, 1.162 ± 0.191 U/mg, and 0.896 ± 0.108 U/mg, respectively. The strain BSR 2 showed the highest lignolytic activity compared to other strains. The optimum temperature for lignolytic enzyme activity of BSR 2 was 30 ℃ and the optimum pH was 7. The lignolytic enzyme activity showed that these bacterial isolates can be a chance to be used as new alternative lignolytic enzyme source in commercial bioconversion process.

scholarly journals عزل وتشخيص البكتريا الملوثة لنهر دجلة في بعض أقضية محافظة واسط

2017 ◽  
Vol 11 (1) ◽  
pp. 37-42
Author(s):  
Helal H. Hays ◽  
Reyad A. Abduljabar ◽  
Hameed Jasim
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Aeromonas Hydrophila ◽  
Enterobacter Cloacae ◽  
Aeromonas Caviae ◽  
Shigella Spp ◽  
Pseudomonas Alcaligenes

تضمنت الدراسة عزل وتشخيص الأنواع والأجناس البكتيرية السائدة في مياه نهر دجلة خلال سنة واحدة ابتداء من شهر حزيران 2015 ولغاية شهر آيار 2016 وبواقع 8 مواقع، توزعت على النحو الأتي: موقعين في كل منطقة تفصل بينهما مسافة 50 متر، في كل من: قضاء العزيزية وناحية الزبيدية وقضاء النعمانية وأما في مدينة الكوت فقد شملت موقعين، احدهما قبل سدة الكوت والأخرى بعد السدة . وتم ذلك بإجراء الاختبارات الكيموحيوية وتأكيد التشخيص باستعمال نظام(Analytical Profile Index )  API20E، تشير نتائج التشخيص إلى وجود 22 عزلة عائدة للأجناس والأنواع الآتية في عينات مياه النهر Aeromnonas veronii و Aeromonas punctata و Bacillus subtilis و Exiguobacterium profoun و Pseudomonas alcaligenes و Enterobacter cloacae و Aeromonas caviae و Pseudomonas fluroescen و Bordetella spp و Photobacterium damala و Aeromonas hydrophila و Acinetobacter bumannii و Serratia liquefciens و Moraxella spp و Buttiaxella agrestis و Shigella spp و Chryseomonas meningosept و Pasteurella spp و Erwinia spp و Escherichia coli و Enterobacterium hormaechei و Escherichia vuleneris. إذ شخصت في فصل الصيف (شهر حزيران 2015)  16 عزلة بنسبة 27.5% وفي فصل الخريف (شهر أيلول) 16 عزلة بنسبة 27.5 % ، أما في فصل الشتاء (شهر كانون الأول) فكانت 12 عزلة ونسبتها 20.6 % وأخيراً في فصل الربيع (شهر آيار 2016) فقد كانت 14 عزلة ونسبتها 24.1 %. وكان الموقع 1 في قضاء العزيزية هو الأكثر تلوث بالبكتريا بسبب مياه المجاري المنزلية وفضلات المواشي ضمن الأنشطة الزراعية. 

scholarly journals Numerical Taxonomy of Pseudomonas alcaligenes, P. pseudoalcaligenes, P. mendocina, P. stutzeri, and Related Bacteria

1989 ◽  
Vol 39 (2) ◽  
pp. 135-144 ◽  
Author(s):  
F. GAVINI ◽  
B. HOLMES ◽  
D. IZARD ◽  
A. BEJI ◽  
A. BERNIGAUD ◽  
...  
Keyword(s):  
Numerical Taxonomy ◽  
Pseudomonas Alcaligenes

Equilibrium and Kinetic Studies for the Biosorption of Aqueous Nickel(II) Ions onto the Pseudomonas alcaligenes Biomass

2010 ◽  
Vol 171-172 ◽  
pp. 41-44
Author(s):  
Xiao Cun Xiao ◽  
Gai Xia Fang ◽  
Er Li Zhao ◽  
Lv Bin Zhai ◽  
Jun Shuai Shi
Keyword(s):  
Industrial Wastewater ◽  
Chemical Interaction ◽  
Kinetic Studies ◽  
Synthetic Wastewater ◽  
Order Kinetic ◽  
Adsorption Capacities ◽  
Order Kinetic Model ◽  
Pseudo Second Order ◽  
Pseudomonas Alcaligenes ◽  
Batch Biosorption

The objective of this study is to assess the environmentaly friendly Ni(II) adsorption from synthetic wastewater using Pseudomonas alcaligenes biomass (PA-2). The ability of PA-2 to remove the Ni(II) ions was investigated by using batch biosorption procedure. The effects such as pH, dosage of biosorbent, Ni(II) initial concentration and sorbate–sorbent contact time and agitating speed on the adsorption capacities of PA-2 were studied. Biosorption equilibriums were rapidly established in about 60 min and the adsorption kinetics followed the pseudo-second order kinetic model. The maximum Ni(II) adsorption capacity determined from Langmuir isotherm were 82.23 mg/g PA-2 at pH 5.0, at 25±2°C and shaker speed 150 rpm, respectively. The carboxyl , hydroxyl and amino groups of the PA-2 were involved in chemical interaction with the Ni(II) ions depicted by Fourier transform infrared spectroscopic (FTIR) results. The study points to the potential of new use of Pseudomonas alcaligenes biomass as an effective biosorbent for the removal of Ni(II) and from environmental and industrial wastewater.

scholarly journals Studies of the polysaccharide fraction from the lipopolysaccharide of Pseudomonas alcaligenes

1974 ◽  
Vol 139 (3) ◽  
pp. 633-643 ◽  
Author(s):  
James A. Lomax ◽  
George W. Gray ◽  
Stephen G. Wilkinson
Keyword(s):  
Gel Filtration ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Methylation Analysis ◽  
Mild Acid Hydrolysis ◽  
Pseudomonas Alcaligenes ◽  
Crude Polysaccharide ◽  
Hydrolysis Of ◽  
Mild Acid

Studies of the lipopolysaccharide of Pseudomonas alcaligenes strain BR 1/2 were extended to the polysaccharide moiety. The crude polysaccharide, obtained by mild acid hydrolysis of the lipopolysaccharide, was fractionated by gel filtration. The major fraction was the phosphorylated polysaccharide, for which the approximate proportions of residues were; glucose (2), rhamnose (0.7), heptose (2–3), galactosamine (1), alanine (1), 3-deoxy-2-octulonic acid (1), phosphorus (5–6). The heptose was l-glycero-d-manno-heptose. The minor fractions from gel filtration contained free 3-deoxy-2-octulonic acid, Pi and PPi. The purified polysaccharide was studied by periodate oxidation, methylation analysis, partial hydrolysis, and dephosphorylation. All the rhamnose and part of the glucose and heptose occur as non-reducing terminal residues. Other glucose residues are 3-substituted, and most heptose residues are esterified with condensed phosphate residues, possibly in the C-4 position. Free heptose and a heptosylglucose were isolated from a partial hydrolysate of the polysaccharide. The location of galactosamine in the polysaccharide was not established, but either the C-3 or C-4 position appears to be substituted and a linkage to alanine was indicated. In its composition, the polysaccharide from Ps. alcaligenes resembles core polysaccharides from other pseudomonads: no possible side-chain polysaccharide was detected.

scholarly journals Molecular and Biochemical Characterization of the xlnD-Encoded 3-Hydroxybenzoate 6-Hydroxylase Involved in the Degradation of 2,5-Xylenol via the Gentisate Pathway in Pseudomonas alcaligenes NCIMB 9867

2005 ◽  
Vol 187 (22) ◽  
pp. 7696-7702 ◽  
Author(s):  
Xiaoli Gao ◽  
Chew Ling Tan ◽  
Chew Chieng Yeo ◽  
Chit Laa Poh
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Molecular Mass ◽  
Electron Donor ◽  
Biochemical Characterization ◽  
Aromatic Substrates ◽  
Gentisate Pathway ◽  
A Subunit ◽  
Pseudomonas Alcaligenes

ABSTRACT The xlnD gene from Pseudomonas alcaligenes NCIMB 9867 (strain P25X) was shown to encode 3-hydroxybenzoate 6-hydroxylase I, the enzyme that catalyzes the NADH-dependent conversion of 3-hydroxybenzoate to gentisate. Active recombinant XlnD was purified as a hexahistidine fusion protein from Escherichia coli, had an estimated molecular mass of 130 kDa, and is probably a trimeric protein with a subunit mass of 43 kDa. This is in contrast to the monomeric nature of the few 3-hydroxybenzoate 6-hydroxylases that have been characterized thus far. Like other 3-hydroxybenzoate 6-hydroxylases, XlnD could utilize either NADH or NADPH as the electron donor. P25X harbors a second 3-hydroxybenzoate 6-hydroxylase II that was strictly inducible by specific aromatic substrates. However, the degradation of 2,5-xylenol and 3,5-xylenol in strain P25X was found to be dependent on the xlnD-encoded 6-hydroxylase I and not the second, strictly inducible 6-hydroxylase II.

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