scholarly journals HBV Genotype Assay

2020 ◽  
Author(s):  
Keyword(s):  
Hbv Genotype ◽  
Genotype Assay

P-A8 Evaluation of LDT HBV Genotype assay and SeqHepB Drug-Resistance Software

2014 ◽  
Vol 67 ◽  
pp. 79
Author(s):  
Chalom Sayada ◽  
Lech Mazur ◽  
Ah-Sung Young ◽  
Michael Mihalov ◽  
Li Ma ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Hbv Genotype ◽  
Genotype Assay

Not HBeAg status but HBV genotype predicts response to IFN in chronic hepatitis B

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
A Erhardt ◽  
A Ludwig ◽  
M Brunetto ◽  
M Popescu ◽  
F van Bömmel ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Hbv Genotype ◽  
Hbeag Status

scholarly journals Diagnosis of α-1-Antitrypsin Deficiency: An Algorithm of Quantification, Genotyping, and Phenotyping

2006 ◽  
Vol 52 (12) ◽  
pp. 2236-2242 ◽  
Author(s):  
Melissa R Snyder ◽  
Jerry A Katzmann ◽  
Malinda L Butz ◽  
Ping Yang ◽  
D Brian Dawson ◽  
...  
Keyword(s):  
Laboratory Testing ◽  
Null Allele ◽  
Melting Curve ◽  
Curve Analysis ◽  
Laboratory Evaluation ◽  
Melting Curve Analysis ◽  
Genotype Assay ◽  
Antitrypsin Deficiency ◽  
Phenotype Analysis ◽  
A1at Deficiency

Abstract Background: Laboratory testing in suspected α-1-antitrypsin (A1AT) deficiency involves analysis of A1AT concentrations and identification of specific alleles by genotyping or phenotyping. The purpose of this study was to define and evaluate a strategy that provides reliable laboratory evaluation of A1AT deficiency. Methods: Samples from 512 individuals referred for A1AT phenotype analysis were analyzed by quantification, phenotype, and genotype. A1AT concentrations were measured by nephelometry. Phenotype analysis was performed by isoelectric focusing electrophoresis. The genotype assay detected the S and Z deficiency alleles by a melting curve analysis. Results: Of the 512 samples analyzed, 2% of the phenotype and genotype results were discordant. Among these 10 discordant results, 7 were attributed to phenotyping errors. On the basis of these data we formulated an algorithm, according to which we analyzed samples by genotyping and quantification assays, with a reflex to phenotyping when the genotype and quantification results were not concordant. Retrospective analyses demonstrated that 4% of samples submitted for genotype and quantitative analysis were reflexed to phenotyping. Of the reflexed samples, phenotyping confirmed the genotype result in 85% of cases. In the remaining 15%, phenotyping provided further information, including identifying rare deficiency alleles and suggesting the presence of a null allele, and allowed for a more definitive interpretation of the genotype result. Conclusions: The combination of genotyping and quantification, with a reflex to phenotyping, is the optimal strategy for the laboratory evaluation of A1AT deficiency.

scholarly journals Molecular Characterization of Coexistence of HBsAg and Anti-HBs in a Patients Infected with HBV Genotype I

2019 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Jing-Hua Fan ◽  
Xiao Li ◽  
Yang Tang ◽  
Xin Lai ◽  
Xi Li ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Hbv Genotype ◽  
Genotype I
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