scholarly journals Immunoglobin G Phospholipid Unit per Milliliter

2020 ◽  
Author(s):  
Keyword(s):  
Immunoglobin G

scholarly journals Quercetin and Idebenone Ameliorate Oxidative Stress, Inflammation, DNA damage, and Apoptosis Induced by Titanium Dioxide Nanoparticles in Rat Liver

Dose-Response ◽  
2018 ◽  
Vol 16 (4) ◽  
pp. 155932581881218 ◽  
Author(s):  
Laila M. Fadda ◽  
Hanan Hagar ◽  
Azza M. Mohamed ◽  
Hanaa M. Ali
Keyword(s):  
Titanium Dioxide ◽  
Histopathological Examination ◽  
Titanium Dioxide Nanoparticles ◽  
Reactive Protein ◽  
Wide Range ◽  
Factor Α ◽  
Immunoglobin G ◽  
Endothelial Growth Factor

Titanium dioxide nanoparticles (TiO2-NPs) are extensively used in a wide range of applications; however, many reports have investigated their nanotoxicological effect at the molecular level either in vitro or in vivo systems. The defensive roles of quercetin (Qur) or idebenone (Id) against the hepatotoxicity induced by TiO2-NPs were evaluated in the current study. The results showed that the coadministration of Qur or Id to rats intoxicated with TiO2-NPs markedly ameliorated the elevation in hepatic malondialdehyde (MDA), serum alanine amino-transferase (ALT), glucose, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), immunoglobin G (IgG), and C-reactive protein (CRP) levels compared to their levels in TiO2-NPs-treated rats. The aforementioned antioxidants also effectively modulated the changes in the levels of serum vascular endothelial growth factor (VEGF), nitric oxide (NO), hepatic DNA breakage, caspase-3, and inhibition of drug metabolizing enzymes (cytochrome P450s; CYP4502E12E1) in rat livers induced by TiO2-NPs toxicity. The histopathological examination of the liver section showed that TiO2-NPs caused severe degeneration of most hepatocytes with an increase in collagen in the portal region, while treatment with the antioxidants in question improved liver architecture. These outcomes supported the use of Qur and Id as protective agents against the hepatotoxicity induced by TiO2-NPs and other hepatotoxic drugs.

scholarly journals Supplementation of Holstein dairy calves fed two levels of crude protein with methionine and lysine

2020 ◽  
Vol 50 (3) ◽  
pp. 442-451
Author(s):  
M. Niroumand ◽  
K. Rezayazdi ◽  
M. Ganjkhanlou
Keyword(s):  
Amino Acid ◽  
Crude Protein ◽  
Average Daily Gain ◽  
Dairy Calves ◽  
Clear Trend ◽  
Reduced Body ◽  
Holstein Calves ◽  
Amino Acid Balance ◽  
Immunoglobin G ◽  
Daily Gain

This study aimed to investigate various levels of crude protein (CP) in starter diets and their supplementation with amino acids (AAs) on efficiency, health, and serum metabolites of dairy calves. Ninety-six newborn Holstein calves were allocated to eight treatments, namely T1) 18% CP unsupplemented starter, T2) T1 supplemented with additional 20% methionine, T3) T1 with additional 20% lysine and 20% methionine, T4) T1 with additional 20% lysine, T5) 22% CP unsupplemented starter, T6) T5 supplemented with additional 20% methionine, T7) T5 supplemented with additional 20% lysine and 20% methionine, and T8) T5 supplemented with additional 20% lysine. Consumption of the starter was not influenced by the level of CP. Calves that received the unsupplemented 22% CP starter had higher average daily gain (ADG) and final weight. But no significant differences among diets were found in feed efficiency. Skeletal growth did not exhibit a clear trend. Calves that received T3 had fewer bouts of diarrhea and reduced body temperature. However, there were no significant dietary effects on immunoglobin G (IgG) or total protein concentration in blood. T1–T3 decreased serum urea concentration. Thus, use of T3 resulted in an improved amino acid balance, and was less expensive than the 22% CP starter.Keywords: amino acid, feed intake, immunity, pre-ruminant calf

scholarly journals Comparison of the Affinity Chromatography and the Ion Exchange Chromatography in the Isolation of Bovine Immunoglobin G

OALib ◽  
2014 ◽  
Vol 01 (06) ◽  
pp. 1-5
Author(s):  
Mrigendra Rajput ◽  
Shimaa M. G. Mansour ◽  
Lyle J. Braun ◽  
Mahmoud Darweesh ◽  
Neelu Thakur ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Affinity Chromatography ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Immunoglobin G

scholarly journals Evaluation of Immunoglobulin G Absorption from Goat Colostrum by Newborn Piglets

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 637
Author(s):  
Silvia Martínez Miró ◽  
Susana Naranjo ◽  
Josefa Madrid ◽  
Miguel José López ◽  
Cristian Jesús Sánchez ◽  
...  
Keyword(s):  
Total Serum ◽  
Control Group ◽  
Artificial Rearing ◽  
Promising Alternative ◽  
Newborn Piglets ◽  
Body Weights ◽  
Feed Supplements ◽  
Suckling Piglets ◽  
Experimental Treatments ◽  
Immunoglobin G

The aim of this study was to evaluate whether piglets absorb immunoglobin G (IgG) from goat colostrum and the potential effects of its ingestion on suckling piglets. Thirty-eight piglets with body weights ranging from 1000 to 1700 g were assigned to one of the three experimental treatments: Control group (C), where piglets were allowed to suckle normally, and porcine and goat groups. The piglets from the last two groups were removed from the sows after birth and received an oral 20 mL dose every 3 h of porcine (PC) or goat colostrum (GC), respectively, during first 12 h of life. Then, they were returned to newly farrowing sows to continue suckling until 20 d. The apparent efficiency of absorption (AEA) of IgG at 12 h was calculated as total serum IgG divided by ingested IgG. No diarrhea or symptoms of intolerance were observed at any time. On day 20, body weight and the number of dead piglets were similar in all three treatments (p > 0.05). At 12 h, the concentration of goat IgG in the serum of piglets fed GC was 8.11 mg/mL. AEA was 20.9% for goat IgG and 26.3% for porcine IgG (p > 0.05). Therefore, goat colostrum seems a promising alternative to study new feed supplements or artificial rearing of newborn piglets.

Immunocytochemical detection of fungi in the roots of Rhododendron

1986 ◽  
Vol 64 (4) ◽  
pp. 718-723 ◽  
Author(s):  
W. C. Mueller ◽  
B. J. Tessier ◽  
L. Englander
Keyword(s):  
Colloidal Gold ◽  
Thin Sections ◽  
Immunocytochemical Detection ◽  
Immunoglobin G

Antisera were prepared from fruiting structures of a Clavaria sp. found growing in the vicinity of Rhododendron plants and from mycelium of Pezizella ericae grown in culture. The antisera were used for the detection and differentiation of fungi in thin sections of roots of Rhododendron by an indirect immunocytochemical procedure using colloidal gold-labelled goat antirabbit immunoglobin G. Three distinct fungal types could be detected in roots: hyphae that reacted only with the Clavaria antiserum; hyphae that reacted only with the Pezizella antiserum; and hyphae that did not react with either antiserum.

scholarly journals Immunological Approaches and Different Strategies for Vaccine Development against SARS-COV-2

2020 ◽  
pp. 91-99
Author(s):  
Muhammed Babakir-Mina
Keyword(s):  
Viral Infection ◽  
Vaccine Development ◽  
Immunological Response ◽  
The Body ◽  
World Health ◽  
Innate And Adaptive Immunity ◽  
Humoral And Cellular Immunity ◽  
The Common ◽  
Health Organization ◽  
Immunoglobin G

Globally, SARS-CoV-2 outbreak is considered as pandemic viral infection by the World Health Organization (WHO). In the immunological response aspect, a very limited understanding has been progressed, mainly innate and adaptive immunity responses toward the virus. SARS-COV-2 causes severe respiratory disease and sometimes ended with the death. The body of the patients has ability to develop the immunity to cure the patient and more importantly both humoral and cellular immunity have studied against SARS-COV-2. There are different immune responses against the viral infection as it has seen in other previous diseases such as SARS-COV and MESR. On the base on immune response detected in recovered patients, scientists have started to develop the vaccines. Moreover, there are different strategies that used by researchers and pharmacological companies to develop vaccines including attenuated or killed viruses, RNA of a spike protein, and vector expressing a particular protein of the virus. The common antibodies have detected to work against SARS-COV-2 in sera of infected or recovered patients are immunoglobin G ( IgG) and immunoglobin M (IgM). The sera of patients recovered from COVID-19, after tittering of immunoglobulins (IgG titer) can be used for either treatment of disease or prophylaxis of infection by SARS-COV-2. This study gives an update on the current immunological approaches and vaccination strategies for the emerging SARS-COV-2, and discusses the challenges and hurdles to overcome for developing efficacious vaccines against this dangerous pathogen.

scholarly journals Controlled Human Infection With Bordetella pertussis Induces Asymptomatic, Immunizing Colonization

2019 ◽  
Vol 71 (2) ◽  
pp. 403-411 ◽  
Author(s):  
Hans de Graaf ◽  
Muktar Ibrahim ◽  
Alison R Hill ◽  
Diane Gbesemete ◽  
Andrew T Vaughan ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Quantitative Polymerase Chain Reaction ◽  
Human Infection ◽  
Wild Type ◽  
Immunological Responses ◽  
Inoculum Dose ◽  
Colony Forming Units ◽  
Community Transmission ◽  
Polymerase Chain ◽  
Immunoglobin G

Abstract Background Bordetella pertussis is among the leading causes of vaccine-preventable deaths and morbidity globally. Human asymptomatic carriage as a reservoir for community transmission of infections might be a target of future vaccine strategies, but has not been demonstrated. Our objective was to demonstrate that asymptomatic nasopharyngeal carriage of Bordetella pertussis is inducible in humans and to define the microbiological and immunological features of presymptomatic infection. Methods Healthy subjects aged 18–45 years with an antipertussis toxin immunoglobin G (IgG) concentration of <20 international units/ml were inoculated intranasally with nonattenuated, wild-type Bordetella pertussis strain B1917. Safety, colonization, and shedding were monitored over 17 days in an inpatient facility. Colonization was assessed by culture and quantitative polymerase chain reaction. Azithromycin was administered from Day 14. The inoculum dose was escalated, aiming to colonize at least 70% of participants. Immunological responses were measured. Results There were 34 participants challenged, in groups of 4 or 5. The dose was gradually escalated from 103 colony-forming units (0% colonized) to 105 colony-forming units (80% colonized). Minor symptoms were reported in a minority of participants. Azithromycin eradicated colonization in 48 hours in 88% of colonized individuals. Antipertussis toxin IgG seroconversion occurred in 9 out of 19 colonized participants and in none of the participants who were not colonized. Nasal wash was a more sensitive method to detect colonization than pernasal swabs. No shedding of Bordetella pertussis was detected in systematically collected environmental samples. Conclusions Bordetella pertussis colonization can be deliberately induced and leads to a systemic immune response without causing pertussis symptoms. Clinical Trials Registration NCT03751514.

Molecular Characterization of Inflammatory Tumors Facilitates Initiation of Effective Therapy

PEDIATRICS ◽  
2021 ◽  
Author(s):  
Franziska Wachter ◽  
Alyaa Al-Ibraheemi ◽  
Maria C. Trissal ◽  
Monica Hollowell ◽  
Steven G. DuBois ◽  
...  
Keyword(s):  
Weight Loss ◽  
Comprehensive Evaluation ◽  
Treatment Options ◽  
Molecular Testing ◽  
Presenting Symptoms ◽  
Oral Targeted Therapy ◽  
Immunoglobin G ◽  
Oncology Center ◽  
Primary Care Pediatricians

Inflammatory myofibroblastic tumor (IMT) is a rare, mesenchymal tumor that has an increased incidence in childhood. Tumors are usually isolated to the chest, abdomen, and retroperitoneum, but metastatic presentations can be seen. Presenting symptoms are nonspecific and include fever, weight loss, pain, shortness of breath, and cough. Approximately 85% of IMTs harbor actionable kinase fusions. The diagnosis can be delayed because of overlapping features with inflammatory disorders, such as elevated inflammatory markers, increased immunoglobin G levels, fever, weight loss, and morphologic similarity with nonmalignant conditions. We present a girl aged 11 years with a TFG-ROS1 fusion–positive tumor of the lung that was initially diagnosed as an immunoglobin G4–related inflammatory pseudotumor. She underwent complete left-sided pneumonectomy and later recurred with widely metastatic disease. We then report the case of a boy aged 9 years with widely metastatic TFG-ROS1 fusion–positive IMT with rapid molecular diagnosis. In both children, there was an excellent response to oral targeted therapy. These cases reveal that rapid molecular testing of inflammatory tumors is not only important for diagnosis but also reveals therapeutic opportunities. Targeted inhibitors produce significant radiologic responses, enabling potentially curative treatment approaches for metastatic ROS1 fusion IMT with previously limited treatment options. Primary care pediatricians and pediatric subspecialists have a crucial role in the early consultation of a pediatric oncology center experienced in molecular diagnostics to facilitate a comprehensive evaluation for children with inflammatory tumors.

scholarly journals Hyperhemolysis Syndrome without Underlying Hematologic Disease

2015 ◽  
Vol 2015 ◽  
pp. 1-3 ◽  
Author(s):  
Lauren Anne Eberly ◽  
Diaa Osman ◽  
Nathaniel Perryman Collins
Keyword(s):  
Laboratory Data ◽  
Rare Occurrence ◽  
Cell Disease ◽  
Hematologic Disease ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test ◽  
Hemolytic Transfusion Reaction ◽  
Life Threatening ◽  
Antibody Screen ◽  
Immunoglobin G

Introduction. Hyperhemolysis is characterized by a life-threatening hemolytic transfusion reaction, with hemoglobin (Hb) and hematocrit (Hct) dropping markedly lower than before transfusion. This phenomenon, commonly described in sickle cell disease, is a rare occurrence in patients without hemoglobinopathies.Case Report. A 55-year-old male presented to the hospital after a motorcycle crash and received 10 units of cross-matched blood for active bleeding. The patient was blood group O, with a negative antibody screen. Ten days later, he represented complaining of dyspnea and was found to have a hematocrit of 12%. The direct antiglobulin test was positive for anti-immunoglobin G and complement. Indirect antiglobulin test was positive for anti-Jka alloantibodies. The presence of Jka antigen was revealed in one unit of previously transfused blood; patient’s RBCs were negative for the Jka antigen. Laboratory data demonstrated findings consistent with DHTR, as well as reticulopenia and elevated ferritin levels. He continued to show signs of active hemolysis, requiring a total of 4 subsequent units of pRBCs. Each transfusion precipitated a drop in Hb and Hct to levels lower than before transfusion; once transfusions were held, the patient slowly recovered.Discussion. Hyperhemolysis in the setting of a DHTR can occur in patients without hematologic disease.

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