scholarly journals Focus of Cellular Alteration

2020 ◽  
Author(s):  
Keyword(s):  
Cellular Alteration

A Cytochemical Study of Glucose-6-Phosphatase (G-6-PASE) in Cells Participating in Atherogenesis of Rabbit Aorta

1975 ◽  
Vol 33 ◽  
pp. 460-461
Author(s):  
Sidney D. Kobernick ◽  
Edna A. Elfont ◽  
Neddra L. Brooks
Keyword(s):  
Lipid Metabolism ◽  
New Zealand ◽  
Aortic Wall ◽  
Rabbit Aorta ◽  
Plaque Formation ◽  
Metabolic Changes ◽  
Atherosclerotic Plaques ◽  
Cytochemical Study ◽  
Cellular Alteration ◽  
New Zealand White Rabbits

This cytochemical study was designed to investigate early metabolic changes in the aortic wall that might lead to or accompany development of atherosclerotic plaques in rabbits. The hypothesis that the primary cellular alteration leading to plaque formation might be due to changes in either carbohydrate or lipid metabolism led to histochemical studies that showed elevation of G-6-Pase in atherosclerotic plaques of rabbit aorta. This observation initiated the present investigation to determine how early in plaque formation and in which cells this change could be observed.Male New Zealand white rabbits of approximately 2000 kg consumed normal diets or diets containing 0.25 or 1.0 gm of cholesterol per day for 10, 50 and 90 days. Aortas were injected jin situ with glutaraldehyde fixative and dissected out. The plaques were identified, isolated, minced and fixed for not more than 10 minutes. Incubation and postfixation proceeded as described by Leskes and co-workers.

Su1588 Helicobacter Hepaticus Infection Promotes Hepatitis and Foci of Cellular Alteration in Farnesoid X Receptor (FXR) Deficient Mice

2012 ◽  
Vol 142 (5) ◽  
pp. S-972-S-973
Author(s):  
Alton G. Swennes ◽  
Alexis García ◽  
Nicola M. Parry ◽  
Kvin Lertpiriyapong ◽  
James G. Fox
Keyword(s):  
Farnesoid X Receptor ◽  
Helicobacter Hepaticus ◽  
Cellular Alteration ◽  
Deficient Mice

Mechanistic insights into cellular alteration of prion by poly‐D‐lysine: the role of H2H3 domain

2011 ◽  
Vol 25 (10) ◽  
pp. 3426-3435 ◽  
Author(s):  
Zhou Xu ◽  
Miquel Adrover ◽  
Annalisa Pastore ◽  
Stéphanie Prigent ◽  
Franck Mouthon ◽  
...  
Keyword(s):  
Cellular Alteration

scholarly journals Cellular Alteration by Interferon: a Virus-Free System for Assaying Interferon

1972 ◽  
Vol 10 (5) ◽  
pp. 896-901 ◽  
Author(s):  
William E. Stewart ◽  
Erik De Clercq ◽  
Pierre De Somer
Keyword(s):  
Free System ◽  
Cellular Alteration

Ectonucleoside Triphosphate Diphosphohydrolase Type 5 (Entpd5)-Deficient Mice Develop Progressive Hepatopathy, Hepatocellular Tumors, and Spermatogenic Arrest

2009 ◽  
Vol 46 (3) ◽  
pp. 491-504 ◽  
Author(s):  
R. Read ◽  
G. Hansen ◽  
J. Kramer ◽  
R. Finch ◽  
L. Li ◽  
...  
Keyword(s):  
Cellular Proliferation ◽  
Hepatocellular Adenoma ◽  
Oval Cell ◽  
Soluble Enzyme ◽  
Wild Type ◽  
Genetic Inactivation ◽  
Cellular Alteration ◽  
Low Incidence ◽  
Proliferation Of Hepatocytes ◽  
Deficient Mice

Ectonucleoside triphosphate diphosphohydrolase type 5 (ENTPD5, also CD39L4) is a soluble enzyme that hydrolyzes purine nucleoside diphosphates. Genetic inactivation of ENTPD5 in mice ( Entpd5-/-) resulted in 2 major histopathologic lesions: hepatopathy and aspermia. The hepatopathy was progressive and characterized by centrilobular hepatocyte hypertrophy, oval cell proliferation, bile staining of Kupffer cells, and hepatocyte degeneration with increasing incidence and severity of degenerative lesions, development of multiple foci of cellular alteration, and hepatocellular neoplasia with age. Greatly increased proliferation of hepatocytes in young adult as well as aged Entpd5-/- mice was demonstrated by Ki67 immunohistochemistry and 5î-bromo-3î-deoxyuridine incorporation. Of 15 Entpd5-/- mice between 44 and 69 weeks of age, all showed foci of cellular alteration in the liver, and at least 6 of 15 developed hepatocellular carcinoma (HCC), hepatocellular adenoma, or both. Significantly, none of these lesions were observed in 13 wild-type Entpd5++ littermates. These findings, combined with the historically low incidence (about 5%) of HCC in mice up to 2 years of age with the same genetic background, strongly suggest that loss of Entpd5 promotes hepatocellular neoplasia in mice. In humans, ENTPD5 has been found to be identical to the PCPH proto-oncogene, and dysregulation of this gene has been demonstrated in some human cancers. This mouse model could contribute to the understanding of the influence of ENTPD5/PCPH on cellular proliferation and neoplasia.

Defining Foci of Cellular Alteration in Short-Term and Medium-Term Rat Liver Tumor Models

1989 ◽  
Vol 17 (4_part_1) ◽  
pp. 561-568 ◽  
Author(s):  
James A. Popp ◽  
Thomas L. Goldsworthy
Keyword(s):  
Rat Liver ◽  
Mechanism Of Action ◽  
Hepatic Tumors ◽  
Medium Term ◽  
Short Term ◽  
Morphological Marker ◽  
Cellular Alteration ◽  
Size Number ◽  
Carcinogenic Potential ◽  
Carcinogenic Effects

Evaluation of foci of cellular alteration is utilized in short-term and mid-term rat liver models to assess chemicals for potential carcinogenic effects. Such foci are morphologically defined and readily identified by a variety of histological stains. No single morphological marker appears capable of identifying all foci because of their phenotypic heterogeneity. Since most rodent hepatocarcinogens induce an increase in the size and/or the number of foci prior to tumor appearance, foci are believed to be precursors to hepatic tumors. The size, number, phenotype and conversion rate of foci to tumors is chemical dependent. Despite certain limitations, the quantitation of rat liver foci provides a useful means to study the mechanism of action of selected chemicals and assess their carcinogenic potential.

Cellular alteration after dilution of cryoprotective solutions used for the vitrification of in vitro-produced bovine embryos

1999 ◽  
Vol 52 (3) ◽  
pp. 515-525 ◽  
Author(s):  
S. Kaidi ◽  
A. Van Langendonckt ◽  
A. Massip ◽  
F. Dessy ◽  
I. Donnay
Keyword(s):  
Bovine Embryos ◽  
Cellular Alteration
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