scholarly journals Candida intermedia

2020 ◽  
Author(s):  
Keyword(s):  
Candida Intermedia

scholarly journals High capacity xylose transport in Candida intermedia PYCC 4715

2003 ◽  
Vol 3 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Márk Gárdonyi ◽  
MÃ¥ns ÖSterberg ◽  
Carla Rodrigues ◽  
Isabel Spencer-Martins ◽  
Bärbel Hahn-Hägerdal
Keyword(s):  
High Capacity ◽  
Xylose Transport ◽  
Candida Intermedia

scholarly journals Three novel species of the anamorphic yeast genus Candida in the Candida intermedia clade found in Japan, Thailand and Taiwan

2011 ◽  
Vol 57 (2) ◽  
pp. 73-81 ◽  
Author(s):  
Takashi Nakase ◽  
Sasitorn Jindamorakot ◽  
Somit Am-In ◽  
Ching-Fu Lee ◽  
Yumi Imanishi ◽  
...  
Keyword(s):  
Novel Species ◽  
Yeast Genus ◽  
Candida Intermedia ◽  
Anamorphic Yeast

scholarly journals Candida duobushaemulonii: An Old But Unreported Pathogen

2020 ◽  
Vol 6 (4) ◽  
pp. 374
Author(s):  
Irene Jurado-Martín ◽  
Cristina Marcos-Arias ◽  
Esther Tamayo ◽  
Andrea Guridi ◽  
Piet W. J. de Groot ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Related Species ◽  
Antifungal Drugs ◽  
Candida Auris ◽  
Closely Related Species ◽  
Identification Methods ◽  
Phenotypic Identification ◽  
Candida Intermedia ◽  
Candida Haemulonii

Candidiasis caused by species of the Candida haemulonii complex (Candida haemulonii and Candida duobushaemulonii) and closely related species, Candida auris and Candida pseudohaemulonii are increasing. These species often show reduced susceptibility to antifungal drugs, such as azoles and amphotericin B or, less frequently, echinocandins. However, conventional phenotypic identification methods are unable to accurately differentiate these species and, therefore, their prevalence may have been underestimated. In this study, 150 isolates that were probably misidentified were reanalyzed using two novel PCR approaches. We found that one isolate previously identified in 1996 as Candida intermedia was C. duobushaemulonii, being one of the oldest isolates of this species described to date. We also found that this isolate had reduced susceptibility to fluconazole, itraconazole, and amphotericin B.

scholarly journals Candida intermedia CBS 141442: A Novel Glucose/Xylose Co-Fermenting Isolate for Lignocellulosic Bioethanol Production

Energies ◽  
2020 ◽  
Vol 13 (20) ◽  
pp. 5363
Author(s):  
Antonio D. Moreno ◽  
Elia Tomás-Pejó ◽  
Lisbeth Olsson ◽  
Cecilia Geijer
Keyword(s):  
Glucose Consumption ◽  
The Novel ◽  
Adaptive Laboratory Evolution ◽  
Xylose Consumption ◽  
Candida Intermedia ◽  
Solid Agar ◽  
Consumption Rates ◽  
Ethanol Yields ◽  
Evolution Experiment ◽  
Wheat Straw Hydrolysate

The present study describes the isolation of the novel strain Candida intermedia CBS 141442 and investigates the potential of this microorganism for the conversion of lignocellulosic streams. Different C. intermedia clones were isolated during an adaptive laboratory evolution experiment under the selection pressure of lignocellulosic hydrolysate and in strong competition with industrial, xylose-fermenting Saccharomyces cerevisiae cells. Isolates showed different but stable colony and cell morphologies when growing in a solid agar medium (smooth, intermediate and complex morphology) and liquid medium (unicellular, aggregates and pseudohyphal morphology). Clones of the same morphology showed similar fermentation patterns, and the C. intermedia clone I5 (CBS 141442) was selected for further testing due to its superior capacity for xylose consumption (90% of the initial xylose concentration within 72 h) and the highest ethanol yields (0.25 ± 0.02 g ethanol/g sugars consumed). Compared to the well-known yeast Scheffersomyces stipitis, the selected strain showed slightly higher tolerance to the lignocellulosic-derived inhibitors when fermenting a wheat straw hydrolysate. Furthermore, its higher glucose consumption rates (compared to S. stipitis) and its capacity for glucose and xylose co-fermentation makes C. intermedia CBS 141442 an attractive microorganism for the conversion of lignocellulosic substrates, as demonstrated in simultaneous saccharification and fermentation processes.

scholarly journals Seleção de leveduras da Região Amazônica para produção de protease extracelular

2006 ◽  
Vol 36 (3) ◽  
pp. 299-306 ◽  
Author(s):  
Kilma Cristiane Silva Neves ◽  
Ana Lúcia Figueiredo Porto ◽  
Maria Francisca Simas Teixeira
Keyword(s):  
Candida Tropicalis ◽  
Candida Intermedia

O objetivo deste trabalho foi identificar 50 leveduras isoladas a partir de diferentes substratos da Região Amazônica e selecionar uma espécie de maior atividade proteolítica. Entre as 26 espécies identificadas predominaram Candida aquatica (12%) e Candida tropicalis (10%). A fermentação submersa foi realizada em Extrato de Malte suplementado com gelatina 1%, Candida intermedia foi a que expressou maior atividade proteolítica, halo = 25 mm e 273 U/mL, crescimento máximo a 30 ºC, viabilidade celular 6,2 x 10(6) UFC, em 48 horas, não demonstrou características de patogenicidade e a melhor densidade do inóculo foi 3%, utilizando-se culturas com 24 horas de crescimento em ágar Malte.

scholarly journals Two glucose/xylose transporter genes from the yeast Candida intermedia: first molecular characterization of a yeast xylose–H+ symporter

2006 ◽  
Vol 395 (3) ◽  
pp. 543-549 ◽  
Author(s):  
Maria José Leandro ◽  
Paula Gonçalves ◽  
Isabel Spencer-Martins
Keyword(s):  
Sequence Data ◽  
High Capacity ◽  
Transport Systems ◽  
Partial Purification ◽  
Facilitated Diffusion ◽  
Xylose Transport ◽  
Candida Intermedia ◽  
Strain Isolation ◽  
Genes Encoding

Candida intermedia PYCC 4715 was previously shown to grow well on xylose and to transport this sugar by two different transport systems: high-capacity and low-affinity facilitated diffusion and a high-affinity xylose–proton symporter, both of which accept glucose as a substrate. Here we report the isolation of genes encoding both transporters, designated GXF1 (glucose/xylose facilitator 1) and GXS1 (glucose/xylose symporter 1) respectively. Although GXF1 was isolated by functional complementation of an HXT-null (where Hxt refers to hexose transporters) Saccharomyces cerevisiae strain, isolation of the GXS1 cDNA required partial purification and micro-sequencing of the transporter, identified by its relative abundance in cells grown on low xylose concentrations. Both genes were expressed in S. cerevisiae and the kinetic parameters of glucose and xylose transport were determined. Gxs1 is the first yeast xylose/glucose–H+ symporter to be characterized at the molecular level. Comparison of its amino acid sequence with available sequence data revealed the existence of a family of putative monosaccharide–H+ symporters encompassing proteins from several yeasts and filamentous fungi.

scholarly journals Brewing and Probiotic Potential Activity of Wild Yeasts Hanseniaspora Uvarum PIT001, Pichia Kluyveri LAR001 and Candida Intermedia ORQ001

2021 ◽  
Author(s):  
Renan Eugênio Araujo Piraine ◽  
Gustavo M Retzlaf ◽  
Vitória S. Gonçalves ◽  
Rodrigo C Cunha ◽  
Fabio Pereira Leivas Leite
Keyword(s):  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Starter Cultures ◽  
Mixed Fermentation ◽  
Candida Intermedia ◽  
Probiotic Potential ◽  
Hanseniaspora Uvarum ◽  
Wide Range ◽  
Pichia Kluyveri

Abstract Non-conventional yeasts can be isolated from a wide range of environmental sources, often found in beverage industry in mixed fermentations, in which the microorganisms’ inoculum usually is not fully known. It is important to know starter cultures, since in addition to favoring reproducibility, other properties can be discovered. Thus, the objective of this work was to identify and characterize yeasts isolated from environment, evaluating their probiotic potential and possible use in brewery. Isolates were obtained from flowers, fruits, leaves and mixed-fermentation beers, being identified by PCR. Yeasts with promising activity were evaluated regarding their growth under different pHs, temperature and presence of organic acids. To explore probiotic potential, in vitro tests were performed of antimicrobial activity and co-aggregation with food pathogens, auto-aggregation, and survival in simulated gastrointestinal tract conditions. In our study, Pichia kluyveri (LAR001), Hanseniaspora uvarum (PIT001) and Candida intermedia (ORQ001) were selected among 20 isolates. P. kluyveri was the only one that tolerated pH 2.5. Lactic acid was not inhibitory, while acetic acid and incubation at 37 °C had a partially inhibitory effect on yeasts growth. All yeasts tolerated α-acids from hops and NaCl up to 1%. It is suggested that isolates are able to adhere to intestinal cells and influence positively the organism in combating pathogens, as they showed auto-aggregation rates above 99% and antagonistic activity to pathogenic bacteria. The yeasts tolerated gastric environment conditions, however were more sensitive to pancreatic conditions. We conclude that isolated non-conventional yeasts showed probiotic potential and promising application in beer fermentation.

scholarly journals Occurrence of Free Base of Phytosphingosine in Candida intermedia (IFO 0761)

1974 ◽  
Vol 38 (6) ◽  
pp. 1263-1264 ◽  
Author(s):  
Akira KIMURA ◽  
Masao KIMURA ◽  
Hiroo OZAKI ◽  
Tatsurokuro TOCHIKURA ◽  
Koichi KOSHIMIZU
Keyword(s):  
Free Base ◽  
Candida Intermedia
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