Esophageal Gland

2020 ◽  
Author(s):  
Keyword(s):  
Esophageal Gland

scholarly journals Immunoreactive Proteins in the Esophageal Gland Cells of Anisakis Simplex Sensu Stricto Detected by MALDI-TOF/TOF Analysis

Genes ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 683
Author(s):  
Lee Robertson ◽  
Susana C. Arcos ◽  
Sergio Ciordia ◽  
Noelia Carballeda-Sanguiao ◽  
María del Carmen Mena ◽  
...  
Keyword(s):  
Sensu Stricto ◽  
Anisakis Simplex ◽  
2D Electrophoresis ◽  
Western Blots ◽  
Gland Cells ◽  
Maldi Tof ◽  
Nematode Parasites ◽  
Esophageal Gland ◽  
Allergenic Potential ◽  
Phylogenetic Origin

In plant and animal nematode parasites, proteins derived from esophageal gland cells have been shown to be important in the host-nematodes relationship but little is known about the allergenic potential of these proteins in the genus Anisakis. Taking into account the increase of anisakiasis and allergies related to these nematodes, immunoreactive properties of gland cell proteins were investigated. Two hundred ventricles were manually dissected from L3 stage larvae of Aniskakis simplex s.s. to allow direct protein analysis. Denaturing gel electrophoresis followed by monochromatic silver staining which revealed the presence of differential (enriched) proteins when compared to total nematode extracts. Such comparison was performed by means of 1D and 2D electrophoresis. Pooled antisera from Anisakis spp.-allergic patients were used in western blots revealing the presence of 13 immunoreactive bands in the ventricular extracts in 1D, with 82 spots revealed in 2D. The corresponding protein bands and spots were excised from the silver-stained gel and protein assignation was made by MALDI-TOF/TOF. A total of 13 (including proteoforms) were unambiguously identified. The majority of these proteins are known to be secreted by nematodes into the external environment, of which three are described as being major allergens in other organisms with different phylogenetic origin and one is an Anisakis simplex allergen.

Endoscopic findings of esophageal gland duct adenoma resected by endoscopic submucosal dissection

2020 ◽  
Vol 92 (4) ◽  
pp. 961-962
Author(s):  
Masashi Yamamoto ◽  
Tsutomu Nishida ◽  
Dai Nakamatsu ◽  
Shiro Adachi ◽  
Masami Inada
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Endoscopic Findings ◽  
Submucosal Dissection ◽  
Esophageal Gland ◽  
Gland Duct

Proteomic profiles of soluble proteins from the esophageal gland in female Meloidogyne incognita

2012 ◽  
Vol 42 (13-14) ◽  
pp. 1177-1183 ◽  
Author(s):  
Xin-Rong Wang ◽  
Yovany A. Moreno ◽  
Han-Rong Wu ◽  
Chao Ma ◽  
Yun-feng Li ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Soluble Proteins ◽  
Esophageal Gland

scholarly journals Cloning and Characterization of an Esophageal-Gland-Specific Pectate Lyase from the Root-Knot Nematode Meloidogyne javanica

2002 ◽  
Vol 15 (6) ◽  
pp. 549-556 ◽  
Author(s):  
Elizabeth A. Doyle ◽  
Kris N. Lambert
Keyword(s):  
Plant Root ◽  
Middle Lamella ◽  
Pectate Lyase ◽  
Meloidogyne Javanica ◽  
Basal Cells ◽  
Class Iii ◽  
Root Knot Nematode ◽  
Ph Optimum ◽  
Pectate Lyases ◽  
Esophageal Gland

Root-knot nematodes (Meloidogyne javanica) are obligate sedentary endoparasites that must penetrate the host root to initiate their life cycle. Many enzymes are secreted by the nematode to facilitate host penetration; required enzymes may include pectate lyases and cellulases. Using differential screening, a class III pectate lyase, Mj-pel-1 (M. javanica pectate lyase 1), was cloned from a library enriched for esophageal gland genes. DNA gel blotting confirmed that the Mj-pel-1 gene was of nematode origin and a member of a small multigene family. In situ hybridization localized the expression of Mj-pel-1 to the basal cells of the esophageal glands, while immunolocalization detected the protein in the esophageal glands as well as on the exterior of the nematode, confirming that the protein is secreted. When MJ-PEL-1 was expressed in Pichia pastoris, the resulting protein was active. The pH optimum of MJ-PEL-1 was 10.0, and the enzyme was five times more active on pectate than on pectin. Like other class III pectate lyases, MJ-PEL-1 also displayed an absolute requirement for Ca2+. The root-knot nematode migrates through the middle lamella of the plant root; therefore, MJ-PEL-1 may be an important enzyme early in the infection process.

Esophageal Gland Duct Adenoma: Immunohistochemical Comparison With the Normal Esophageal Gland and Ultrastractural Analysis

2007 ◽  
Vol 31 (3) ◽  
pp. 469-475 ◽  
Author(s):  
Oi Harada ◽  
Hiroyoshi Ota ◽  
Tsutomu Katsuyama ◽  
Eiko Hidaka ◽  
Katsuhiko Ishizaka ◽  
...  
Keyword(s):  
Esophageal Gland ◽  
Gland Duct

scholarly journals A Profile of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Root-knot Nematode Meloidogyne incognita

2003 ◽  
Vol 16 (5) ◽  
pp. 376-381 ◽  
Author(s):  
Guozhong Huang ◽  
Bingli Gao ◽  
Tom Maier ◽  
R. Allen ◽  
Eric L. Davis ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Secretory Proteins ◽  
Cdna Clones ◽  
Root Knot Nematode ◽  
Long Distance ◽  
Gland Cells ◽  
Esophageal Gland ◽  
Genes Encoding ◽  
Nematode Parasitism ◽  
Parasitism Genes

Identifying parasitism genes encoding proteins secreted from a nematode's esophageal gland cells and injected through its stylet into plant tissue is the key to understanding the molecular basis of nematode parasitism of plants. Meloidogyne incognita parasitism genes were cloned by microaspirating the cytoplasm from the esophageal gland cells of different parasitic stages to provide mRNA to create a gland cell-specific cDNA library by long-distance reverse-transcriptase polymerase chain reaction. Of 2,452 cDNA clones sequenced, deduced protein sequences of 185 cDNAs had a signal peptide for secretion and, thus, could have a role in root-knot nematode parasitism of plants. High-throughput in situ hybridization with cDNA clones encoding signal peptides resulted in probes of 37 unique clones specifically hybridizing to transcripts accumulating within the subventral (13 clones) or dorsal (24 clones) esophageal gland cells of M. incognita. In BLASTP analyses, 73% of the predicted proteins were novel proteins. Those with similarities to known proteins included a pectate lyase, acid phosphatase, and hypothetical proteins from other organisms. Our cell-specific analysis of genes encoding secretory proteins provided, for the first time, a profile of putative parasitism genes expressed in the M. incognita esophageal gland cells throughout the parasitic cycle.

scholarly journals Esophageal Adenocarcinoma Originating from the Esophageal Gland Duct

2019 ◽  
Vol 58 (6) ◽  
pp. 883-884 ◽  
Author(s):  
Mitsuhiro Kono ◽  
Yasuaki Nagami ◽  
Masahiko Ohsawa ◽  
Yasuhiro Fujiwara
Keyword(s):  
Esophageal Adenocarcinoma ◽  
Esophageal Gland ◽  
Gland Duct

Esophageal Gland Duct Adenoma

2011 ◽  
Vol 45 (Suppl 1) ◽  
pp. S45
Author(s):  
Yoonjung Kim ◽  
Yang-Soon Park ◽  
Jei So Bang ◽  
Ji Yeon Kim ◽  
Young-Hyeh Ko ◽  
...  
Keyword(s):  
Esophageal Gland ◽  
Gland Duct
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