scholarly journals CAMP-Dependent Protein Kinase Type I-Alpha Regulatory Subunit

2020 ◽  
Author(s):  
Keyword(s):  
Protein Kinase ◽  
Regulatory Subunit ◽  
Type I ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Dependent Protein

Regulation of lung epithelial cell morphology by cAMP-dependent protein kinase type I isozyme

2001 ◽  
Vol 280 (6) ◽  
pp. L1282-L1289 ◽  
Author(s):  
Stephanie E. Porter ◽  
Lori D. Dwyer-Nield ◽  
Alvin M. Malkinson
Keyword(s):  
Protein Kinase ◽  
Cell Junctions ◽  
Actin Binding ◽  
Regulatory Subunit ◽  
Type I ◽  
Stable Transfection ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Lung Epithelial ◽  
Dependent Protein

Cell shape is mediated in part by the actin cytoskeleton and the actin-binding protein vinculin. These proteins in turn are regulated by protein phosphorylation. We assessed the contribution of cAMP-dependent protein kinase A isozyme I (PKA I) to lung epithelial morphology using the E10/E9 sibling cell lines. PKA I concentration is high in flattened, nontumorigenic E10 cells but low in their round E9 transformants. PKA I activity was lowered in E10 cells by stable transfection with a dominant negative RIα mutant of the PKA I regulatory subunit and was raised in E9 cells by stable transfection with a wild-type Cα catalytic subunit construct. Reciprocal changes in morphology ensued. E10 cells became rounder and grew in colonies, their actin microfilaments were disrupted, and vinculin localization at cell-cell junctions was diminished. The converse occurred in E9 cells on elevating their PKA I content. Demonstration that PKA I is responsible for the dichotomy in these cellular behaviors suggests that manipulating PKA I concentrations in lung cancer would provide useful adjuvant therapy.

scholarly journals PrKX Is a Novel Catalytic Subunit of the cAMP-dependent Protein Kinase Regulated by the Regulatory Subunit Type I

1999 ◽  
Vol 274 (9) ◽  
pp. 5370-5378 ◽  
Author(s):  
Bastian Zimmermann ◽  
John A. Chiorini ◽  
Yuliang Ma ◽  
Robert M. Kotin ◽  
Friedrich W. Herberg
Keyword(s):  
Protein Kinase ◽  
Catalytic Subunit ◽  
Regulatory Subunit ◽  
Type I ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Dependent Protein
Sign in / Sign up

Export Citation Format

Share Document