scholarly journals Campylobacter upsaliensis

2020 ◽  
Author(s):  
Keyword(s):  
Campylobacter Upsaliensis

scholarly journals High-Resolution Genotyping of Campylobacter upsaliensis Strains Originating from Three Continents

2004 ◽  
Vol 42 (8) ◽  
pp. 3441-3448 ◽  
Author(s):  
P. Lentzsch ◽  
B. Rieksneuwohner ◽  
L. H. Wieler ◽  
H. Hotzel ◽  
I. Moser
Keyword(s):  
High Resolution ◽  
Campylobacter Upsaliensis

scholarly journals First Case Report of Fatal Sepsis Due to Campylobacter upsaliensis

2014 ◽  
Vol 53 (2) ◽  
pp. 713-715 ◽  
Author(s):  
Itaru Nakamura ◽  
Nami Omori ◽  
Ayaka Umeda ◽  
Kiyofumi Ohkusu ◽  
Tetsuya Matsumoto
Keyword(s):  
Case Report ◽  
Rare Case ◽  
Pcr Amplification ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Content Type ◽  
First Case ◽  
Campylobacter Upsaliensis ◽  
Fatal Sepsis ◽  
The 16S Rrna Gene

We encountered a rare case of severe fatal infection in a 70-year-old woman due toCampylobacter upsaliensis, identified by PCR amplification and sequencing analysis of the 16S rRNA gene using DNA extracted from the isolates. To our knowledge, fatal sepsis due to this organism has never been described to date.

Isolation and purification of a Campylobacter upsaliensis autolysin

1999 ◽  
Vol 45 (1) ◽  
pp. 23-30
Author(s):  
Somchai Santiwatanakul ◽  
Noel R Krieg
Keyword(s):  
Escherichia Coli ◽  
Molecular Mass ◽  
Micrococcus Luteus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Isolation And Purification ◽  
Whole Cells ◽  
Sds Page ◽  
Autolytic Activity ◽  
Campylobacter Upsaliensis

Autolytic activity in the soluble and sediment fractions of sonicates of the spiral and the coccoid form of Campylobacter upsaliensis could not be demonstrated by native (nondenaturing) polyacrylamide gel electrophoresis (PAGE). Autolysins were detected, however, by using denaturing sodium dodecyl sulfate (SDS) - PAGE gels containing either purified Escherichia coli peptidoglycan or whole cells of Micrococcus luteus (Micrococcus lysodeikticus) as the turbid substrate, with subsequent renaturation by treatment with Triton X-100 buffer. In renaturing gels that contained Escherichia coli peptidoglycan, 14 putative autolytic bands ranging from 200 to 12 kDa were detected. In similar gels containing whole cells of M. luteus, only a single band appeared with a molecular mass of 34 kDa. This band corresponded to one of the bands present in the gels containing Escherichia coli peptidoglycan. This common autolysin was isolated by adsorbing it from Campylobacter upsaliensis soluble fractions onto M. luteus cells and then subjecting these cells to renaturing SDS-PAGE in gels containing Escherichia coli peptidoglycan. The 34-kDa autolysin differed from a single 51-kDa autolysin unique to the M. luteus cells, and when isolated from an SDS-PAGE gel, was pure when tested by isoelectric focusing. The N-terminal amino acid sequence analysis showed the first 15 amino acids of the 34-kDa autolysin to have 67% identity to a part of antigenic protein PEB4 of Campylobacter jejuni. The purified autolysin was used to immunize rabbits and the antibodies produced precipitated autolytic activity from cell lysates. The specificity of the antibodies was shown by Western blotting: only a single specific band occurred, with a molecular mass of 34 kDa, and thus it seems unlikely that the 34-kDa autolysin was derived from any of the other autolysins that were detected.Key words: autolysin, Campylobacter upsaliensis, zymogram, murein hydrolase.

Campylobacter upsaliensis, C. sputorum sputorum and C. concisus as Common Causes of Diarrhoea in Swedish Children

1995 ◽  
Vol 27 (2) ◽  
pp. 187-188 ◽  
Author(s):  
Gun-Britt Lindblom ◽  
Eva Sjögren ◽  
Jane Hansson-Westerberg ◽  
Bertil Kaijser
Keyword(s):  
Common Causes ◽  
Campylobacter Upsaliensis

scholarly journals Selective medium for thermophilic campylobacters including Campylobacter upsaliensis.

1993 ◽  
Vol 46 (9) ◽  
pp. 829-831 ◽  
Author(s):  
S T Aspinall ◽  
D R Wareing ◽  
P G Hayward ◽  
D N Hutchinson
Keyword(s):  
Selective Medium ◽  
Campylobacter Upsaliensis

scholarly journals The role of Campylobacter spp. in chronic enteropathy in dogs

2019 ◽  
Vol 88 (3) ◽  
pp. 341-347
Author(s):  
Miloš Vávra ◽  
Gabriela Bořilová ◽  
Michal Fusek ◽  
Vojtěch Gabriel ◽  
Václav Ceplecha ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Test Results ◽  
Research Subjects ◽  
Chronic Enteropathy ◽  
Gastrointestinal Problems ◽  
Stool Samples ◽  
Campylobacter Upsaliensis ◽  
The Relationship ◽  
Campylobacter Spp

The aim of the study was to identify Campylobacter species in a group of patients with chronic gastrointestinal problems and to investigate the relationship between the presence of Campylobacter spp. in stool samples and as well as the severity of chronic enteropathy. Twenty-six dogs with chronic gastrointestinal problems were included in the prospective study. Each research subject had their stomach, duodenum, ileum, and colon examined endoscopically. A histopathological examination of the obtained biopsy samples was then performed, excluding other potential diseases. Stool samples were collected and then examined for the presence of Campylobacter spp. To evaluate the relationship between Campylobacter spp. occurrence and the intensity of chronic enteropathy, patients were divided into two groups; animals in the first group presented with no to mild inflammation whereas research subjects in the second group suffered from moderate to severe inflammation. Subsequently, the patients were divided based on positive or negative test results for Campylobacter spp. cultures. No significant relationship between the presence of Campylobacter spp. in stool samples and chronic enteropathy was found. In contrast to other previously published papers, our study showed a lower occurrence of Campylobacter upsaliensis.

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