scholarly journals Murine Monoclonal Antibody 225

2020 ◽  
Author(s):  
Keyword(s):  
Monoclonal Antibody ◽  
Murine Monoclonal Antibody

Partial Inhibition of Platelet Aggregation and Fibrinogen Binding by a Murine Monoclonal Antibody to GPIIIa: Requirement for Antibody Bivalency

1994 ◽  
Vol 72 (06) ◽  
pp. 964-972 ◽  
Author(s):  
Jeffery L Kutok ◽  
Barry S Coller
Keyword(s):  
Monoclonal Antibody ◽  
Platelet Aggregation ◽  
Murine Monoclonal Antibody ◽  
Platelet Glycoprotein ◽  
Igg Antibody ◽  
Surface Receptors ◽  
Partial Inhibition ◽  
Fibrinogen Binding ◽  
Igg Binding ◽  
Agonist Concentration

SummaryWe produced a murine monoclonal antibody, 7H2, and localized its epitope to one or more small regions on platelet glycoprotein (GP) Ilia. 7H2-IgG and 7H2-F(ab’)2 completely inhibit platelet aggregation and fibrinogen binding at low agonist concentrations, but only partially inhibit aggregation and fibrinogen binding at high agonist concentrations; 7H2-Fab has no effect on aggregation or fibrinogen binding at any agonist concentration. 7H2-IgG binds to the entire platelet population as judged by flow cytometry. At near saturating concentrations, ∼40,000 7H2-IgG antibody molecules bind per platelet. In contrast, ∼80,000 7H2 Fab molecules bind per platelet, suggesting that 7H2-IgG binding is bivalent. 7H2 was unable to inhibit fibrinogen binding to purified, immobilized GPIIb/IIIa. These data indicate that the bivalent binding of 7H2 to GPIIIa is required for its partial inhibition of fibrinogen binding to platelets, perhaps through dimerization of GPIIb/IIIa surface receptors (or more complex GPIIb/IIIa redistribution triggered by 7H2 binding) resulting in limited accessibility of fibrinogen to its binding site(s).

scholarly journals Clinical evaluation of tumor targeting with the anticarcinoembryonic antigen murine monoclonal antibody fragment, MN-14 F(ab)2

Cancer ◽  
1996 ◽  
Vol 78 (1) ◽  
pp. 157-168 ◽  
Author(s):  
Malik Juweid ◽  
Robert M. Sharkey ◽  
Thomas M. Behr ◽  
Lawrence C. Swayne ◽  
Robert Dunn ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Clinical Evaluation ◽  
Tumor Targeting ◽  
Antibody Fragment ◽  
Murine Monoclonal Antibody

UN1, a murine monoclonal antibody recognizing a novel human thymic antigen

1994 ◽  
Vol 44 (2) ◽  
pp. 73-82 ◽  
Author(s):  
Pierfrancesco Tassone ◽  
Heather Bond ◽  
Patrizia Bonelli ◽  
Franca Tuccillo ◽  
Giuliana Valerio ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Murine Monoclonal Antibody

Characterization of a murine monoclonal antibody specific for human early lymphohemopoietic cells

1984 ◽  
Vol 9 (1) ◽  
pp. 9-20 ◽  
Author(s):  
Fabio Malavasi ◽  
Federico Caligaris-Cappio ◽  
Claudio Milanese ◽  
Paolo Dellabona ◽  
Patricia Richiardi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Murine Monoclonal Antibody
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