scholarly journals Cryopreserved Cell

2020 ◽  
Author(s):  
Keyword(s):  
Cryopreserved Cell

scholarly journals Cryopreservation of human cancers conserves tumour heterogeneity for single-cell multi-omics analysis

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Sunny Z. Wu ◽  
Daniel L. Roden ◽  
Ghamdan Al-Eryani ◽  
Nenad Bartonicek ◽  
Kate Harvey ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
High Throughput ◽  
Single Cells ◽  
Cellular Heterogeneity ◽  
Tumour Heterogeneity ◽  
Fresh Tissue ◽  
Human Cancers ◽  
Cryopreserved Cell ◽  
Single Cell Rna Sequencing

Abstract Background High throughput single-cell RNA sequencing (scRNA-Seq) has emerged as a powerful tool for exploring cellular heterogeneity among complex human cancers. scRNA-Seq studies using fresh human surgical tissue are logistically difficult, preclude histopathological triage of samples, and limit the ability to perform batch processing. This hindrance can often introduce technical biases when integrating patient datasets and increase experimental costs. Although tissue preservation methods have been previously explored to address such issues, it is yet to be examined on complex human tissues, such as solid cancers and on high throughput scRNA-Seq platforms. Methods Using the Chromium 10X platform, we sequenced a total of ~ 120,000 cells from fresh and cryopreserved replicates across three primary breast cancers, two primary prostate cancers and a cutaneous melanoma. We performed detailed analyses between cells from each condition to assess the effects of cryopreservation on cellular heterogeneity, cell quality, clustering and the identification of gene ontologies. In addition, we performed single-cell immunophenotyping using CITE-Seq on a single breast cancer sample cryopreserved as solid tissue fragments. Results Tumour heterogeneity identified from fresh tissues was largely conserved in cryopreserved replicates. We show that sequencing of single cells prepared from cryopreserved tissue fragments or from cryopreserved cell suspensions is comparable to sequenced cells prepared from fresh tissue, with cryopreserved cell suspensions displaying higher correlations with fresh tissue in gene expression. We showed that cryopreservation had minimal impacts on the results of downstream analyses such as biological pathway enrichment. For some tumours, cryopreservation modestly increased cell stress signatures compared to freshly analysed tissue. Further, we demonstrate the advantage of cryopreserving whole-cells for detecting cell-surface proteins using CITE-Seq, which is impossible using other preservation methods such as single nuclei-sequencing. Conclusions We show that the viable cryopreservation of human cancers provides high-quality single-cells for multi-omics analysis. Our study guides new experimental designs for tissue biobanking for future clinical single-cell RNA sequencing studies.

Changes in Cryopreserved Cell Nuclei Serve as Indicators of Processes during Freezing and Thawing

Langmuir ◽  
2018 ◽  
Vol 35 (23) ◽  
pp. 7496-7508 ◽  
Author(s):  
Irena Kratochvílová ◽  
Olga Kopečná ◽  
Alena Bačíková ◽  
Eva Pagáčová ◽  
Iva Falková ◽  
...  
Keyword(s):  
Cell Nuclei ◽  
Freezing And Thawing ◽  
Cryopreserved Cell

Allogeneic Cell Therapy for the Treatment of Liver Disease

2005 ◽  
Vol 15 (2) ◽  
pp. 178-184
Author(s):  
John W. Ludlow ◽  
Andrew T. Bruce ◽  
Michael J. Kulik ◽  
Sonya O. Meheux ◽  
Darell W. McCoy ◽  
...  
Keyword(s):  
Liver Disease ◽  
Cell Therapy ◽  
Severe Liver ◽  
Human Organs ◽  
Allogeneic Cell Therapy ◽  
Cryopreserved Cell ◽  
Liver Functions ◽  
Severe Liver Disease ◽  
Liver Replacement ◽  
Organ Replacement

The scarcity of human organs available for transplantation is clearly evident. Efforts to maximize the use of available organs and to increase the number of donors have increased the number of transplantations performed, but at a rate that remains far behind the rate of growth of the waiting list. Thus, the likelihood of a patient with severe liver disease receiving a liver replacement is decreasing. In order to offer treatment to most patients with liver disease, alternatives to whole-organ replacement must be found. Cell-based treatments, in which suspensions of liver cells are injected into patients with liver failure and reconstitute the patient's liver functions, may be that alternative. Here, we report on a regulatory-compliant process for the production of a cryopreserved cell therapy product that yields viable, metabolically active hepatocytes that can be infused directly into patients with the goal of reconstituting liver function.

scholarly journals Cryopreserved cell-laden alginate microgel bioink for 3D bioprinting of living tissues

2019 ◽  
Vol 12 ◽  
pp. 61-70 ◽  
Author(s):  
O. Jeon ◽  
Y.B. Lee ◽  
T.J. Hinton ◽  
A.W. Feinberg ◽  
E. Alsberg
Keyword(s):  
3D Bioprinting ◽  
Cryopreserved Cell ◽  
Living Tissues

Induction of Somatic Embryos and Plantlets from Cryopreserved Cell Cultures of White Spruce (Picea glauca)

1988 ◽  
Vol 132 (5) ◽  
pp. 529-539 ◽  
Author(s):  
K.K. Kartha ◽  
L.C. Fowke ◽  
N.L. Leung ◽  
K.L. Caswell ◽  
I. Hakman
Keyword(s):  
Cell Cultures ◽  
Picea Glauca ◽  
Somatic Embryos ◽  
White Spruce ◽  
Cryopreserved Cell

scholarly journals Characterizing Cannabinoid CB2 Receptor Ligands Using DiscoveRx PathHunter™ β-Arrestin Assay

2008 ◽  
Vol 14 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Debra Mcguinness ◽  
Asra Malikzay ◽  
Richard Visconti ◽  
Karen Lin ◽  
Marvin Bayne ◽  
...  
Keyword(s):  
Cb2 Receptor ◽  
Receptor Ligands ◽  
Camp Assay ◽  
Cryopreserved Cell ◽  
Random Library ◽  
A Cell ◽  
Multiple Cell ◽  
Screening Platform ◽  
Protein Complementation ◽  
Camp Levels

The authors have characterized a set of cannabinoid CB2 receptor ligands, including triaryl bis sulfone inverse agonists, in a cell-based receptor/β-arrestin interaction assay (DiscoveRx PathHunter™). The results were compared with results using a competitive ligand binding assay, and with effects on forskolin-stimulated cAMP levels (PerkinElmer LANCE™). The authors show good correlation between the 3 assay systems tested, with the β-arrestin protein complementation assay exhibiting a more robust signal than the cAMP assay for cannabinoid CB2 agonists. Further assay validation shows that DiscoveRx PathHunter™ HEK293 CB2 β-arrestin assay can be carried out from cryopreserved cell suspensions, eliminating variations caused by the need for multiple cell pools during live cell screening campaigns. These results, and the authors' results evaluating a test set of random library compounds, validate the use of ligand-induced interaction between the human cannabinoid CB2 receptor and β-arrestin as an appropriate and valuable screening platform for compounds specific for the cannabinoid CB2 receptor. ( Journal of Biomolecular Screening 2009:49-58)

Retention of the capacity to produce plants from protoplasts in cryopreserved cell lines of rice (Oryza sativa L.)

1991 ◽  
Vol 10 (4) ◽  
pp. 171-174 ◽  
Author(s):  
E. G. M. Meijer ◽  
F. van Iren ◽  
E. Schrijnemakers ◽  
L. A. M. Hensgens ◽  
M. van Zijderveld ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Cell Lines ◽  
Oryza Sativa L ◽  
Cryopreserved Cell
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