Bovine Cartilage

2020 ◽  
Author(s):  
Keyword(s):  
Bovine Cartilage

scholarly journals Characterization of collagen types XII and XIV from fetal bovine cartilage.

1992 ◽  
Vol 267 (28) ◽  
pp. 20093-20099
Author(s):  
S.L. Watt ◽  
G.P. Lunstrum ◽  
A.M. McDonough ◽  
D.R. Keene ◽  
R.E. Burgeson ◽  
...  
Keyword(s):  
Collagen Types ◽  
Fetal Bovine ◽  
Bovine Cartilage

First experiences with irradiated bovine cartilage in orbital surgery Experimental and clinical study

Orbit ◽  
1988 ◽  
Vol 7 (1) ◽  
pp. 27-29 ◽  
Author(s):  
N. Delle Noci ◽  
F. Mininni ◽  
C. Iaculli
Keyword(s):  
Clinical Study ◽  
Orbital Surgery ◽  
Bovine Cartilage

scholarly journals A potential key role for alpha-haemolysin ofStaphylococcus aureusin mediating chondrocyte death in septic arthritis

2018 ◽  
Vol 7 (7) ◽  
pp. 457-467 ◽  
Author(s):  
I. D. M. Smith ◽  
K. M. Milto ◽  
C. J. Doherty ◽  
S. G. B. Amyes ◽  
A. H. R. W. Simpson ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Septic Arthritis ◽  
Cartilage Damage ◽  
Laser Scanning Microscopy ◽  
Cartilage Explants ◽  
Confocal Laser ◽  
Dead Cell ◽  
Chondrocyte Viability ◽  
Chondrocyte Death ◽  
Bovine Cartilage

ObjectivesStaphylococcus aureus (S. aureus) is the most commonly implicated organism in septic arthritis, a condition that may be highly destructive to articular cartilage. Previous studies investigating laboratory and clinical strains of S. aureus have demonstrated that potent toxins induced significant chondrocyte death, although the precise toxin or toxins that were involved was unknown. In this study, we used isogenic S. aureus mutants to assess the influence of alpha (Hla)-, beta (Hlb)-, and gamma (Hlg)-haemolysins, toxins considered important for the destruction of host tissue, on in situ bovine chondrocyte viability.MethodsBovine cartilage explants were cultured with isogenic S. aureus mutants and/or their culture supernatants. Chondrocyte viability was then assessed within defined regions of interest in the axial and coronal plane following live- and dead-cell imaging using the fluorescent probes 5-chloromethylfluorescein diacetate and propidium iodide, respectively, and confocal laser-scanning microscopy.ResultsHla-producing mutants caused substantial chondrocyte death compared with the toxin-deficient control (Hla-Hlb-Hlg-), whilst mutants producing Hlb and Hlg in the absence of Hla induced minimal chondrocyte death. Coronal studies established that Hla-induced chondrocyte death started in the superficial zone of cartilage and spread to deeper layers, whereas Hlb and Hlg toxins were without significant effect.ConclusionThis study identified Hla as a highly potent S. aureus toxin that caused rapid chondrocyte death in bovine cartilage, with other toxins or metabolic products produced by the bacteria playing a minor role. The identification of Hla in mediating chondrocyte death may assist in the development of therapeutic strategies aimed at reducing the extent of cartilage damage during and after an episode of septic arthritis. Cite this article: I. D. M. Smith, K. M. Milto, C. J. Doherty, S. G. B. Amyes, A. H. R. W. Simpson, A. C. Hall. A potential key role for alpha-haemolysin of Staphylococcus aureus in mediating chondrocyte death in septic arthritis. Bone Joint Res 2018;7:457–467. DOI: 10.1302/2046-3758.77.BJR-2017-0165.R1.

scholarly journals Immature bovine cartilage wear by fatigue failure and delamination

2020 ◽  
Vol 107 ◽  
pp. 109852 ◽  
Author(s):  
Krista M. Durney ◽  
Courtney A. Shaeffer ◽  
Brandon K. Zimmerman ◽  
Robert J. Nims ◽  
Sevan Oungoulian ◽  
...  
Keyword(s):  
Fatigue Failure ◽  
Cartilage Wear ◽  
Bovine Cartilage

Bovine cartilage in correction of nasal deformities

1933 ◽  
Vol 43 (12) ◽  
pp. 976-979 ◽  
Author(s):  
Philip S. Stout
Keyword(s):  
Bovine Cartilage

Processed Irradiated Bovine Cartilage for Nasal Reconstruction

1988 ◽  
Vol 20 (5) ◽  
pp. 499 ◽  
Author(s):  
Robert A. Ersek ◽  
Antoine G. Delerm
Keyword(s):  
Nasal Reconstruction ◽  
Bovine Cartilage

Tissue Shear Deformation Stimulates Proteoglycan and Protein Biosynthesis in Bovine Cartilage Explants

2001 ◽  
Vol 395 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Moonsoo Jin ◽  
Eliot H. Frank ◽  
Thomas M. Quinn ◽  
Ernst B. Hunziker ◽  
Alan J. Grodzinsky
Keyword(s):  
Shear Deformation ◽  
Protein Biosynthesis ◽  
Cartilage Explants ◽  
Bovine Cartilage

scholarly journals Chondrocyte-mediated catabolism of aggrecan: aggrecanase-dependent cleavage induced by interleukin-1 or retinoic acid can be inhibited by glucosamine

1998 ◽  
Vol 335 (1) ◽  
pp. 59-66 ◽  
Author(s):  
John D. SANDY ◽  
Dan GAMETT ◽  
Vivian THOMPSON ◽  
Christie VERSCHAREN
Keyword(s):  
Retinoic Acid ◽  
Core Protein ◽  
Interleukin 1 ◽  
Cartilage Explants ◽  
Biosynthetic Activity ◽  
Fragment Analysis ◽  
Analysis System ◽  
Chondrosarcoma Cell Line ◽  
Bovine Cartilage ◽  
Aggrecan Core Protein

A rat chondrosarcoma cell line and bovine cartilage explants have been used to study the control of aggrecan degradation by chondrocytes treated with interleukin-1 (IL-1) or retinoic acid (RA). Aggrecan fragment analysis with anti-neo-epitope antibodies suggests that aggrecanase (an as yet unidentified enzyme) is the only aggrecan-degrading proteinase active in these cultures. With rat cells, aggrecanase converts the aggrecan core protein into two major G1-domain-bearing products (60 kDa with a C-terminal Glu-373, and 220 kDa with a C-terminal Glu-1459). Both products were quantified on a standardized Western analysis system with a G1-specific antibody. Immunoblots were analysed by scanning densitometry and the sensitivity, linearity and reproducibility of the assay were established. With rat cells the aggrecanase response to IL-1 was optimal at about 2 mM glutamine, but was progressively inhibited at higher concentrations, with about 90% inhibition at 10 mM glutamine. Such inhibition by glutamine was not, however, observed with bovine explants. On the other hand, marked inhibition of aggrecanase-dependent cleavage was observed with both rat cells and bovine explants when d(+)-glucosamine was included at concentrations above 2 mM. Inhibition was apparently not due to cytotoxicity or interference with IL-1 signalling, since biosynthetic activity was not inhibited and inhibition of the aggrecanase response was also obtained when RA was used as the catabolic stimulator. Possible mechanisms for the inhibition of the aggrecanase response by glucosamine in chondrocytes treated with IL-1 or RA are discussed.

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