scholarly journals Human Monoclonal Antibody B11-hCG Beta Fusion Protein CDX-1307

2020 ◽  
Author(s):  
Keyword(s):  
Monoclonal Antibody ◽  
Fusion Protein ◽  
Human Monoclonal Antibody

scholarly journals Bacterial Expression of a Human Monoclonal Antibody-Alkaline Phosphatase Conjugate Specific for Entamoeba histolytica

2004 ◽  
Vol 11 (1) ◽  
pp. 216-218 ◽  
Author(s):  
Hiroshi Tachibana ◽  
Masataka Takekoshi ◽  
Xun-Jia Cheng ◽  
Yuta Nakata ◽  
Tsutomu Takeuchi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Entamoeba Histolytica ◽  
Human Monoclonal Antibody ◽  
Bacterial Expression ◽  
Human Antibody ◽  
Gene Encoding ◽  
E Coli ◽  
Fab Fragments

ABSTRACT We previously produced human monoclonal antibody Fab fragments specific to Entamoeba histolytica in Escherichia coli. In order to use these Fab fragments for diagnostic purposes, an expression vector to produce a fusion protein of Fab and alkaline phosphatase (PhoA) in E. coli was designed and constructed. The E. coli PhoA gene was fused to the 3′ terminus of the gene encoding the heavy-chain Fd region. The kappa and Fd genes from a previously prepared antibody clone, CP33, which is specific for the 260-kDa lectin of E. histolytica, were used as human antibody genes. When the fusion protein of CP33 and PhoA was incubated with paraformaldehyde-fixed trophozoites of E. histolytica and developed with a substrate, the trophozoites appeared to be stained. These results demonstrate the feasibility of bacterial expression of a human monoclonal antibody-PhoA conjugate specific for E. histolytica and that the antibody can be used to detect E. histolytica antigen without the use of chemically conjugated secondary antibodies.

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