scholarly journals MUC4 Gene

2020 ◽  
Author(s):  
Keyword(s):  
Muc4 Gene

scholarly journals Effects of RNAi-mediated MUC4 gene silencing on the proliferation and migration of human pancreatic carcinoma BxPC-3 cells

2016 ◽  
Vol 36 (6) ◽  
pp. 3449-3455 ◽  
Author(s):  
Yong Li ◽  
Changqiang Wu ◽  
Tianwu Chen ◽  
Juanjuan Zhang ◽  
Gang Liu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Pancreatic Carcinoma ◽  
And Migration ◽  
Muc4 Gene ◽  
Proliferation And Migration

Identification and structure of a mouse homologue to the human MUC4 gene

2000 ◽  
Vol 118 (4) ◽  
pp. A596 ◽  
Author(s):  
Allen E. Bartman ◽  
Laurie L. Shekels ◽  
Ruth E. Anway ◽  
Ilene K. Gipson ◽  
Rob Moccia ◽  
...  
Keyword(s):  
Mouse Homologue ◽  
Muc4 Gene

scholarly journals miR-581-Related Single Nucleotide Polymorphism, rs2641726, Located in MUC4 Gene, is Associated with Gastric Cancer Incidence

2018 ◽  
Vol 34 (3) ◽  
pp. 347-351 ◽  
Author(s):  
Fariba Nabatchian ◽  
Mahdis Rahimi Naiini ◽  
Afshin Moradi ◽  
Hossein Tabatabaeian ◽  
Negin Hoghoughi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Single Nucleotide Polymorphism ◽  
Cancer Incidence ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Muc4 Gene

scholarly journals Nucleotide variability and linkage disequilibrium patterns in the porcine MUC4 gene

BMC Genetics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 57 ◽  
Author(s):  
Ming Yang ◽  
Bin Yang ◽  
Xueming Yan ◽  
Jing Ouyang ◽  
Weihong Zeng ◽  
...  
Keyword(s):  
Linkage Disequilibrium ◽  
Nucleotide Variability ◽  
Muc4 Gene

R455 – Effect of Glucocorticoid on the MUC4 Gene

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P197-P197
Author(s):  
Yong-Dae Kim ◽  
Chang Hoon Bae ◽  
Woo Hyun-Jae ◽  
Heung-Man Lee
Keyword(s):  
Epithelial Cells ◽  
Nasal Polyp ◽  
Nasal Polyps ◽  
Topical Steroid ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Glycoprotein Synthesis ◽  
Mucin Genes ◽  
Mucin Glycoprotein ◽  
Muc4 Gene

Problem Among the airway mucin genes, the MUC4 gene is an important gene in its response to inflammatory diseases of the upper airway. However, the expression and regulation of the MUC4 gene in the nasal polyps remains unclear. The purpose of this study was to evaluate the expression of MUC4 mRNA and synthesis of mucin glycoprotein in the nasal polyps before and after treatment with a topical steroid in vivo and in vitro. Methods Nasal polyps were obtained from 20 patients with chronic rhinosinusitis and were subsequently cultured. The level of MUC4 mRNA was measured by reverse-transcription polymerase chain reaction, and the amount of the MUC4 mucin glycoprotein was estimated by the enzyme-linked immunosorbent assay method. Results The expression of MUC4 mRNA was found to be significantly higher in the nasal polyps than in the inferior turbinate (P < .05). The addition of interleukin (IL)-1beta and lipopolysaccharide (LPS) increased the expression of MUC4 mRNA and mucin glycoprotein synthesis in cultured nasal polyp epithelial cells. Treatment with glucocorticoid inhibited the expression of MUC4 mRNA in the nasal polyps; it also inhibited the expression of IL-1beta and LPS-induced MUC4 mRNA and mucin glycoprotein synthesis in cultured nasal polyp epithelial cells. The inhibitory effects of glucocorticoid were restored by treatment with a glucocorticoid receptor antagonist (RU-486). Conclusion These results suggest that the MUC4 gene is expressed in the nasal polyps and that glucocorticoid can control the expression of the MUC4 gene and mucin glycoprotein synthesis. Significance Based on these findings, we conclude that the MUC4 gene is one of the important mucin genes expressed in the nasal polyps and that glucocorticoid can control the expression of MUC4 genes and mucin glycoprotein synthesis.

scholarly journals The Antioxidant Alpha-Lipoic Acid Inhibits Proliferation and Invasion of Human Gastric Cancer Cells via Suppression of STAT3-Mediated MUC4 Gene Expression

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Yu Yang ◽  
Erhu Fang ◽  
Jiajun Luo ◽  
Hongxue Wu ◽  
Yue Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Cancer Cells ◽  
Human Gastric Cancer ◽  
Alpha Lipoic Acid ◽  
Gastric Cancer Cells ◽  
Muc4 Expression ◽  
Cancer Tissues ◽  
Muc4 Gene ◽  
Proliferation And Invasion

Background. Metastasis and invasion are the main causes of mortality in gastric cancer. To improve the treatment of gastric cancer, the development of effective and innovative antitumor agents toward invasion and proliferation is needed. Alpha-lipoic acid (ALA), a naturally occurring thiol antioxidant, showed antiproliferative and cytotoxic effects on several cancers. So it is feasible to explore whether ALA can be used to inhibit proliferation and invasion in human gastric cancer. Methods. The expression of MUC4 in human gastric cancer tissues was assayed by immunohistochemistry. Then, we performed in vitro cell proliferation and invasion analysis to explore the antitumor effect of ALA using AGS, BGC-823, and MKN-28 cells. To further explore the mechanism of ALA-mediated downregulation of MUC4, we cotransfected human gastric cancer cells with STAT3 siRNA and STAT3 overexpression construct. ChIP assays were carried out to find the relationship between MUC4 and STAT3. Results. We found that the MUC4 gene was strongly expressed in human gastric cancer tissues. Meanwhile, ALA reduced proliferation and invasion of human gastric cancer cells by suppressing MUC4 expression. We also found that STAT3 was involved in the inhibition of MUC4 by ALA. Mechanistically, ALA suppressed MUC4 expression by inhibiting STAT3 binding to the MUC4 promoter region. Conclusion. ALA inhibits both proliferation and invasion of gastric cancer cells by suppression of STAT3-mediated MUC4 gene expression.

Cloning and characterization of the 5′ flanking region of the sialomucin complex/rat Muc4 gene: promoter activity in cultured cells

2000 ◽  
Vol 349 (2) ◽  
pp. 641-649 ◽  
Author(s):  
Shari A. PRICE-SCHIAVI ◽  
Aymee PEREZ ◽  
Roy BARCO ◽  
Kermit L. CARRAWAY
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Promoter Activity ◽  
Regulatory Elements ◽  
Tissue Specific ◽  
Normal Rat ◽  
Hct 116 ◽  
Flanking Region ◽  
Sialomucin Complex ◽  
Muc4 Gene

Sialomucin complex (SMC/Muc4) is a heterodimeric glycoprotein complex consisting of a mucin subunit ascites sialoglycoprotein-1 (ASGP-1) and a transmembrane subunit (ASGP-2), which is aberrantly expressed on the surfaces of a variety of tumour cells. SMC is transcribed from a single gene, translated into a large polypeptide precursor, and further processed to yield the mature ASGP-1/ASGP-2 complex. SMC has complex spatial and temporal expression patterns in the normal rat, suggesting that it has complex regulatory mechanisms. A crude exon/intron map of the 5ʹ regions of the SMC/Muc4 gene generated from clones isolated from a normal rat liver genomic DNA library reveals that this gene has a small first exon comprising the 5ʹ untranslated region and signal peptide, followed by a large intron. The second exon appears to be large, comprising the 5ʹ unique region and a large part (probably all) of the tandem repeat domain. This structure is strikingly similar to that reported for the human MUC4 gene. Using PCR-based DNA walking, 2.4 kb of the 5ʹ-flanking region of the SMC/Muc4 gene was cloned and characterized. Promoter-pattern searches yielded multiple motifs commonly found in tissue-specific promoters. Reporter constructs generated from this 2.4 kb fragment demonstrate promoter activity in primary rat mammary epithelial cells (MEC), the human colon tumour cell line HCT-116, and the human lung carcinoma cell line NCI-H292, but not in COS-7 cells, suggesting epithelial cell specificity. Deletion constructs of this sequence transfected into rat MEC or HCT-116 cells demonstrate greatly varying levels of activity, suggesting that there are positive and negative, as well as tissue-specific, regulatory elements in this sequence. Taken together, these data suggest that the rat SMC/Muc4 promoter has been identified, that it is tissue- (epithelial cell-) specific, and that there are both positive and negative, as well as tissue-specific, regulatory elements in the sequence.

scholarly journals Analysis of porcine MUC4 gene as a candidate gene for prolificacy QTL on SSC13 in an Iberian × Meishan F2 population

BMC Genetics ◽  
2011 ◽  
Vol 12 (1) ◽  
pp. 93 ◽  
Author(s):  
Ingrid Balcells ◽  
Anna Castelló ◽  
Anna Mercadé ◽  
José L Noguera ◽  
Amanda Fernández-Rodríguez ◽  
...  
Keyword(s):  
Candidate Gene ◽  
F2 Population ◽  
Muc4 Gene
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