scholarly journals Double-Stranded RNA Binding Domain

2020 ◽  
Author(s):  
Keyword(s):  
Rna Binding ◽  
Binding Domain ◽  
Double Stranded Rna ◽  
Rna Binding Domain

scholarly journals Two functionally distinct RNA-binding motifs in the regulatory domain of the protein kinase DAI.

1995 ◽  
Vol 15 (1) ◽  
pp. 358-364 ◽  
Author(s):  
S R Green ◽  
L Manche ◽  
M B Mathews
Keyword(s):  
Amino Acid ◽  
Protein Kinase ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Alpha Helix ◽  
Binding Domain ◽  
Single Amino Acid ◽  
Double Stranded Rna ◽  
C Terminus ◽  
Rna Binding Domain

The RNA-binding domain of the protein kinase DAI, the double-stranded RNA inhibitor of translation, contains two repeats of a motif that is also found in a number of other RNA-binding proteins. This motif consists of 67 amino acid residues and is predicted to contain a positively charged alpha helix at its C terminus. We have analyzed the effects of equivalent single amino acid changes in three conserved residues distributed over each copy of the motif. Mutants in the C-terminal portion of either repeat were severely defective, indicating that both copies of the motif are essential for RNA binding. Changes in the N-terminal and central parts of the motif were more debilitating if they were made in the first motif than in the second, suggesting that the first motif is the more important for RNA binding and that the second motif is structurally more flexible. When the second motif was replaced by a duplicate of the first motif, the ectopic copy retained its greater sensitivity to mutation, implying that the two motifs have distinct functions with respect to the process of RNA binding. Furthermore, the mutations have the same effect on the binding of double-stranded RNA and VA RNA, consistent with the existence of a single RNA-binding domain for both activating and inhibitory RNAs.

scholarly journals Identification of the gene encoding a type 1 RNase H with an N-terminal double-stranded RNA binding domain from a psychrotrophic bacterium

FEBS Journal ◽  
2007 ◽  
Vol 274 (14) ◽  
pp. 3715-3727 ◽  
Author(s):  
Takashi Tadokoro ◽  
Hyongi Chon ◽  
Yuichi Koga ◽  
Kazufumi Takano ◽  
Shigenori Kanaya
Keyword(s):  
Rna Binding ◽  
Rnase H ◽  
Binding Domain ◽  
Gene Encoding ◽  
Double Stranded Rna ◽  
Psychrotrophic Bacterium ◽  
Rna Binding Domain

scholarly journals The Double-stranded RNA Binding Domain of the Vaccinia Virus E3L Protein Inhibits Both RNA- and DNA-induced Activation of Interferon β

2009 ◽  
Vol 284 (38) ◽  
pp. 25471-25478 ◽  
Author(s):  
Jean-Baptiste Marq ◽  
Stéphane Hausmann ◽  
Jeremy Luban ◽  
Daniel Kolakofsky ◽  
Dominique Garcin
Keyword(s):  
Vaccinia Virus ◽  
Rna Binding ◽  
Binding Domain ◽  
Interferon Β ◽  
Double Stranded Rna ◽  
Rna Binding Domain ◽  
Rna And Dna

scholarly journals Structure of the double-stranded RNA-binding domain of the protein kinase PKR reveals the molecular basis of its dsRNA-mediated activation

1998 ◽  
Vol 17 (18) ◽  
pp. 5458-5465 ◽  
Author(s):  
Sambasivarao Nanduri ◽  
Bruce W. Carpick ◽  
Yanwu Yang ◽  
Bryan R.G. Williams ◽  
Jun Qin
Keyword(s):  
Protein Kinase ◽  
Molecular Basis ◽  
Rna Binding ◽  
Binding Domain ◽  
Double Stranded Rna ◽  
Rna Binding Domain
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