scholarly journals Mouse Erythroleukemia

2020 ◽  
Author(s):  
Keyword(s):  
Mouse Erythroleukemia

scholarly journals Serum factors can modulate the developmental clock of gamma- to beta-globin gene switching in somatic cell hybrids.

1993 ◽  
Vol 13 (8) ◽  
pp. 4844-4851 ◽  
Author(s):  
G Zitnik ◽  
Q Li ◽  
G Stamatoyannopoulos ◽  
T Papayannopoulou
Keyword(s):  
Globin Gene ◽  
Cumulative Number ◽  
Beta Globin ◽  
Serum Factors ◽  
Beta Globin Gene ◽  
Cell Divisions ◽  
Growth Promoting ◽  
Gene Switching ◽  
Mouse Erythroleukemia

The fusion of human fetal erythroid (HFE) cells with mouse erythroleukemia (MEL) cells produces stable synkaryons (HFE x MEL) which can be monitored for extended periods of time in culture. Initially these hybrids express a human fetal globin program (gamma >> beta), but after weeks or months in culture, they switch to an adult pattern of globin expression (beta >> gamma). The rate at which hybrids switch to the adult phenotype is roughly dependent on the gestational age of the fetal erythroid cells used in the fusion, suggesting that the rate of switching in vitro may be determined by a developmental clock type of mechanism, possibly involving the cumulative number of divisions experienced by the human fetal cells. To investigate whether the number or rate of cell divisions postfusion can influence the rate of switching, we monitored the rate of switching in hybrids from independent fusions under growth-promoting (serum-replete) and growth-suppressing (serum-deprived) conditions. We found that hybrids grown under serum-deprived or serumless conditions switched more rapidly to adult globin expression than did their counterparts in serum-replete conditions. Neither the number of cumulative cell divisions nor time in culture per se predicted the rate of switching in vitro. Our data suggest that factors present in serum either retard switching of hybrids by their presence or promote switching by their absence, indicating that globin switching in vitro can be modulated by the environment; however, once switching in HFE x MEL hybrids is complete, serum factors cannot reverse this process.

Regulation of human globin gene expression in mouse erythroleukemia � human fibroblast hybrid cells

1982 ◽  
Vol 8 (2) ◽  
pp. 163-178 ◽  
Author(s):  
Devi Vembu ◽  
Neal S. Young ◽  
Marcia Willing ◽  
Eve Church ◽  
Linda Sanders-Haigh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Fibroblast ◽  
Globin Gene ◽  
Hybrid Cells ◽  
Globin Gene Expression ◽  
Mouse Erythroleukemia

Rapid induction of polyadenylated H1 histone mRNAs in mouse erythroleukemia cells is regulated by c-myc

1989 ◽  
Vol 9 (6) ◽  
pp. 2332-2340
Author(s):  
G H Cheng ◽  
A I Skoultchi
Keyword(s):  
Terminal Differentiation ◽  
Histone Genes ◽  
H1 Histone ◽  
Rapid Induction ◽  
Histone Mrnas ◽  
Cellular Genes ◽  
Erythroleukemia Cells ◽  
Multistep Process ◽  
Murine Erythroleukemia ◽  
Mouse Erythroleukemia

Chemically induced differentiation of murine erythroleukemia cells is a multistep process involving a precommitment period in which exposure to inducer leads to cells that are irreversibly committed to terminal differentiation. Certain changes in the expression of cellular proto-oncogenes are an important feature of the precommitment phase. We have identified two H1 histone genes that are rapidly induced during this period. Unlike most histone genes, these two H1 genes encode polyadenylated mRNAs with long 3' untranslated regions. To investigate the relationship between induction of the H1 mRNAs and changes in proto-oncogene expression, we studied two independent series of mouse erythroleukemia cell lines that are inhibited from differentiating because of deregulated expression of transfected copies of c-myc or c-myb. The results showed that induction of the H1 mRNAs was negatively regulated by c-myc. The two H1 histone genes are among the first examples of specific cellular genes that are regulated by c-myc. The timing of their induction suggests that they may play an important role in achieving commitment to terminal differentiation.

scholarly journals Induction of cyclic AMP-dependent phosphorylation in cytosol of differentiating mouse erythroleukemia cell line 179

FEBS Letters ◽  
1982 ◽  
Vol 142 (2) ◽  
pp. 210-214 ◽  
Author(s):  
Carol A. Emler ◽  
Joseph A. Fontana ◽  
Fred R. Butcher
Keyword(s):  
Cyclic Amp ◽  
Cell Line ◽  
Erythroleukemia Cell ◽  
Mouse Erythroleukemia

Induction of mouse erythroleukemia cells by microinjection of inducing compound

1981 ◽  
Vol 131 (1) ◽  
pp. 73-77 ◽  
Author(s):  
E DIACUMAKOS ◽  
L KILLOS ◽  
L LEE ◽  
W ANDERSON
Keyword(s):  
Erythroleukemia Cells ◽  
Mouse Erythroleukemia
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