scholarly journals Early E1A Protein

2020 ◽  
Author(s):  
Keyword(s):  
E1a Protein

scholarly journals Induction of transcription factor AP-1 by adenovirus E1A protein and cAMP.

1989 ◽  
Vol 3 (12a) ◽  
pp. 1991-2002 ◽  
Author(s):  
U Muller ◽  
M P Roberts ◽  
D A Engel ◽  
W Doerfler ◽  
T Shenk
Keyword(s):  
Transcription Factor ◽  
Adenovirus E1a ◽  
E1a Protein

Vector expression of adenovirus type 5 E1a proteins: evidence for E1a autoregulation

1985 ◽  
Vol 5 (10) ◽  
pp. 2684-2696
Author(s):  
D H Smith ◽  
D M Kegler ◽  
E B Ziff
Keyword(s):  
Amino Acid ◽  
Simian Virus 40 ◽  
Adenovirus Type ◽  
Simian Virus ◽  
Mutant Form ◽  
Amino Acid Residues ◽  
Reading Frame ◽  
Acid Protein ◽  
Dna Replication Origin ◽  
E1a Protein

We transiently expressed adenovirus type C E1a proteins in wild-type or mutant form from plasmid vectors which have different combinations of E1a and simian virus 40 enhancer elements and which contain the DNA replication origin of SV40 and can replicate in COS 7 cells. We measured the levels of E1a mRNA encoded by the vectors and the transition regulation properties of the protein products. Three vectors encoded equivalent levels of E1a mRNA in COS 7 cells: (i) a plasmid encoding the wt 289-amino acid E1a protein (this complemented the E1a deletion mutant dl312 for early region E2a expression under both replicative and nonreplicative conditions); (ii) a vector for the wt 243-amino acid E1a protein (this complemented dl312 weakly and only under conditions of high multiplicities of dl312); (iii) a mutant, pSVXL105, in which amino acid residues-38 through 44 of the 289-amino acid E1a protein (which includes two highly conserved residues) are replaced by 3 novel amino acids (this also complemented dl312 efficiently). A fourth vector, mutant pSVXL3 with which linker substitution shifts the reading frame to encode a truncated 70-amino acid fragment from the amino terminus of the 289-amino acid protein, was unable to complement dl312. Surprisingly, pSVXL3 overexpressed E1a mRNA approximately 30-fold in COS 7 cells in comparison with the other vectors. The pSVXL3 overexpression could be reversed by cotransfection with a wt E1a vector. We suggest that wt E1a proteins regulate the levels of their own mRNAs through the recently described transcription repression functions of the 289- and 243-amino acid E1a protein products and that pSVXL3 fails to autoregulate negatively.

scholarly journals Cells differentiating into neuroectoderm undergo apoptosis in the absence of functional retinoblastoma family proteins.

1995 ◽  
Vol 129 (3) ◽  
pp. 779-788 ◽  
Author(s):  
R S Slack ◽  
I S Skerjanc ◽  
B Lach ◽  
J Craig ◽  
K Jardine ◽  
...  
Keyword(s):  
Cell Death ◽  
P19 Cells ◽  
Adenovirus E1a ◽  
Genes Encoding ◽  
Extensive Cell Death ◽  
Extensive Cell ◽  
Early Mouse ◽  
Dying Cells ◽  
Related Proteins ◽  
E1a Protein

The retinoblastoma (RB) protein is present at low levels in early mouse embryos and in pluripotent P19 embryonal carcinoma cells; however, the levels of RB rise dramatically in neuroectoderm formed both in embryos and in differentiating cultures of P19 cells. To investigate the effect of inactivating RB and related proteins p107 and p130, we transfected P19 cells with genes encoding mutated versions of the adenovirus E1A protein that bind RB and related proteins. When these E1A-expressing P19 cells were induced to differentiate into neuroectoderm, there was a striking rise in the expression of c-fos and extensive cell death. The ultrastructural and biochemical characteristics of the dying cells were indicative of apoptosis. The dying cells were those committed to the neural lineages because neurons and astrocytes were lost from differentiating cultures. Cell death was dependent on the ability of the E1A protein to bind RB and related proteins. Our results suggest that proteins of the RB family are essential for the development of the neural lineages and that the absence of functional RB activity triggers apoptosis of differentiating neuroectodermal cells.

scholarly journals Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.

1991 ◽  
Vol 88 (12) ◽  
pp. 5124-5128 ◽  
Author(s):  
N. Horikoshi ◽  
K. Maguire ◽  
A. Kralli ◽  
E. Maldonado ◽  
D. Reinberg ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Direct Interaction ◽  
Tata Box ◽  
Adenovirus E1a ◽  
Transcription Factor Iid ◽  
E1a Protein

Transcription activation by the adenovirus E1a protein

Nature ◽  
1989 ◽  
Vol 338 (6210) ◽  
pp. 39-44 ◽  
Author(s):  
James W. Lillie ◽  
Michael R. Green
Keyword(s):  
Transcription Activation ◽  
Adenovirus E1a ◽  
E1a Protein

scholarly journals The Adenovirus E1A Protein Is a Potent Coactivator for Thyroid Hormone Receptors

1999 ◽  
Vol 13 (7) ◽  
pp. 1119-1129 ◽  
Author(s):  
Gunilla M. Wahlström ◽  
Björn Vennström ◽  
Maria Bondesson Bolin
Keyword(s):  
Thyroid Hormone ◽  
Hormone Receptors ◽  
Thyroid Hormone Receptors ◽  
Adenovirus E1a ◽  
E1a Protein
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