scholarly journals Fluorescent Treponemal Antibody Absorption

2020 ◽  
Author(s):  
Keyword(s):  
Antibody Absorption

scholarly journals Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test.

1979 ◽  
Vol 55 (2) ◽  
pp. 105-108
Author(s):  
E F Hunter ◽  
R M McKinney ◽  
S E Maddison ◽  
D D Cruce
Keyword(s):  
Staining Procedure ◽  
Double Staining ◽  
Antibody Absorption

scholarly journals ANTIGENIC ANALYSIS OF CERTAIN GROUP B ARTHROPOD-BORNE VIRUSES BY ANTIBODY ABSORPTION

1960 ◽  
Vol 111 (1) ◽  
pp. 21-32 ◽  
Author(s):  
D. H. Clarke
Keyword(s):  
Yellow Fever ◽  
Vaccine Strain ◽  
Yellow Fever Virus ◽  
Geographical Area ◽  
Specific Antigen ◽  
Antigenic Structure ◽  
Antigenic Analysis ◽  
West Nile ◽  
Antibody Absorption ◽  
Group B

A method for carrying out antibody absorption studies for antigenic analysis of group B arthropod-borne (arbor) viruses is described and examples of homologous and heterologous absorption curves are presented. Evidence that antigenic structure can be a stable property was obtained with three strains of West Nile virus isolated from different hosts in different countries over a period of years. Comparative studies with viruses of the Japanese B-St. Louis-West Nile subgroup indicate that each virus contains a completely specific antigen as well as one or more cross-reactive components. Strains of yellow fever virus isolated in America were shown to lack an antigen present in strains of African origin although no differences were found between isolates from the same geographical area. The attenuated 17 D vaccine strain of yellow fever was found to have acquired an additional antigen not present in the unadapted parent or in other strains tested. However, alteration in pathogenicity for man was not found to be necessarily attended by any antigenic modification, as shown by the antigenic identity of the French neurotropic vaccine strain with its pantropic parent.

scholarly journals Clinical Performance of the BioPlex 2200 Syphilis Total & RPR Assay at a Tertiary Medical Center with a High Rate of Syphilis

2018 ◽  
Vol 57 (1) ◽  
Author(s):  
Merih T. Tesfazghi ◽  
Neil W. Anderson ◽  
Ann M. Gronowski ◽  
Melanie L. Yarbrough
Keyword(s):  
Clinical Performance ◽  
Medical Center ◽  
High Volume ◽  
High Rate ◽  
Rapid Plasma Reagin ◽  
Antibody Absorption ◽  
Traditional Algorithm ◽  
Syphilis Screening ◽  
Manual Methods ◽  
Positive Population

ABSTRACT Manual treponemal and nontreponemal serologic testing has historically been used for the diagnosis of syphilis. This approach is simple and reproducible but labor intensive. Recently, the FDA cleared the fully automated BioPlex 2200 Syphilis Total & RPR assay for the detection of treponemal and nontreponemal antibodies. We evaluated the clinical performance of this assay at a tertiary medical center with a high syphilis prevalence. Prospective consecutively collected (n = 400) and known RPR-positive (n = 100) specimens were compared using predicate manual rapid plasma reagin (RPR) and fluorescent treponemal antibody absorption (FTA) methods and the BioPlex 2200 Syphilis Total & RPR assay. Positive and negative percent agreements (PPA and NPA, respectively) between the assays were calculated. The PPA and NPA between the manual and BioPlex 2200 RPR results for the prospective population were 85% (17/20; 95% confidence interval [CI], 69% to 100%) and 98% (373/380; 95% CI, 97% to 99%), respectively. The PPA for the manual RPR-positive population was 88% (88/100; 95% CI, 82% to 94%). Overall, the manual and BioPlex 2200 RPR titers demonstrated 78% (99/127) concordance within ±1 dilution and 94% (120/127) within ±2 dilutions. An interpretation of the syphilis serologic profile using the traditional algorithm showed a concordance of 99.5% in the prospective population and 85% in the manual RPR-positive cohort. The performance of the BioPlex 2200 Syphilis Total & RPR assay is comparable to those of manual methods. The high NPA of this assay combined with the ability to automate a historically labor-intensive assay is an appealing attribute for syphilis screening in a high-volume laboratory.

scholarly journals Evaluation of reagin screen, a new serological test for syphilis

1976 ◽  
Vol 4 (2) ◽  
pp. 145-150
Author(s):  
J D Dyckman ◽  
R D Wende ◽  
D Gantenbein ◽  
R P Williams
Keyword(s):  
Research Laboratory ◽  
Serological Test ◽  
Venereal Disease ◽  
The Other ◽  
Screening Tests ◽  
Venereal Disease Research Laboratory ◽  
Rapid Plasma Reagin ◽  
Disease Research ◽  
Antibody Absorption

A total of 1,020 serum and plasma specimens were tested using the Venereal Disease Research Laboratory (VDRL), Rapid Plasma Reagin (RPR) card, Reagin Screen (RST) and Fluorescent Treponemal Antibody-Absorption (FTA-ABS) tests. In 257 normal patients, all screening tests were nonreactive; the FTA-ABS test was reactive for one patient. In 588 patients with treated and untreated syphilis, the RST results were 91.7% in agreement with the VDRL and RPR results. In 175 patients with diseases that cause biological false reactions, the RST was 94% in agreement with the other screening tests. The titer of the RST was within one dilution of the corresponding VDRL titer in 91.7% of the 360 speciments tested and within one dilution of the RPR titer in 96.9% of 358 specimens quantitated by both tests.

scholarly journals Serological evidence of Rickettsia parkeri as the etiological agent of rickettsiosis in Uruguay

2009 ◽  
Vol 51 (6) ◽  
pp. 337-339 ◽  
Author(s):  
Ismael A. Conti-Díaz ◽  
Jonas Moraes-Filho ◽  
Richard C. Pacheco ◽  
Marcelo B. Labruna
Keyword(s):  
Spotted Fever ◽  
Clinical Manifestations ◽  
The United States ◽  
Immunofluorescence Assay ◽  
Etiological Agent ◽  
Serological Evidence ◽  
Rickettsia Parkeri ◽  
Rickettsia Rickettsii ◽  
Antibody Absorption ◽  
Mild Fever

We report three new rickettsiosis human cases in Uruguay. The three clinical cases presented clinical manifestations similar to previous reported cases of Rickettsia parkeri in the United States; that is mild fever (< 40 ºC), malaise, headache, rash, inoculation eschar at the tick bite site, regional lymphadenopathy, and no lethality. Serological antibody-absorption tests with purified antigens of R. parkeri and Rickettsia rickettsii, associated with immunofluorescence assay indicated that the patients in two cases were infected by R. parkeri. Epidemiological and clinical evidences, coupled with our serological analysis, suggest that R. parkeri is the etiological agent of human cases of spotted fever in Uruguay, a disease that has been recognized in that country as cutaneous-ganglionar rickettsiosis.

Immunoglobulin specificity for the fluorescent treponemal antibody-absorption test conjugate

1976 ◽  
Vol 4 (4) ◽  
pp. 338-342
Author(s):  
E F Hunter ◽  
S E Maddison ◽  
S A Larsen ◽  
M B Felker ◽  
J C Feeley
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Absorption Test ◽  
Human Immunoglobulin G ◽  
Antibody Absorption

Quantitative indirect immunofluorescence tests have demonstrated the predominance of immunoglobulin G antibody in most stages of syphilis. A class-specific anti-human immunoglobulin G conjugate is recommended to replace the present fluorescent treponemal antibody-absorption test conjugate. This would allow further definition and standardization of the fluorescent treponemal antibody-absorption test.

scholarly journals Human Hardjo leptospirosis detected in the Slovak Republic by using serum antibody absorption test

2019 ◽  
Vol 120 (03) ◽  
pp. 171-176
Author(s):  
P. Bakoss ◽  
H. Hudecova ◽  
J. Jarekova ◽  
J. Perzelova
Keyword(s):  
Slovak Republic ◽  
Serum Antibody ◽  
Absorption Test ◽  
Antibody Absorption
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