scholarly journals Tetradecanoylphorbol Acetate

2020 ◽  
Author(s):  
Keyword(s):  
Tetradecanoylphorbol Acetate

Effect of Minor Components of Virgin Olive Oil on Topical Antiinflammatory Assays

2000 ◽  
Vol 55 (9-10) ◽  
pp. 814-819 ◽  
Author(s):  
Rocío de la Puerta ◽  
Eugenia Martínez-Domínguez ◽  
Valentina Ruíz-Gutiérrez
Keyword(s):  
Olive Oil ◽  
Virgin Olive Oil ◽  
Polar Compounds ◽  
Biological Properties ◽  
Variable Degree ◽  
Marker Enzyme ◽  
Unsaponifiable Fraction ◽  
Antiinflammatory Effect ◽  
Reference Drug ◽  
Tetradecanoylphorbol Acetate

Abstract Interest in the health-promoting effects of virgin olive oil, an important part of the “Mediterranean diet”, prompted us to determine the antiinflammatory effects of erythrodiol, β-sitosterol and squalene, identified as major components of the so-called “unsaponifiable fraction” of virgin olive oil, as well as of the phenolic compounds from the “polar fraction”: oleuropein, tyrosol, hydroxytyrosol and caffeic acid. Their activities were compared to those of both, total unsaponifiable and polar fractions. This study was designed to analyse the antiinflammatory effect of these specific compounds from virgin olive oil on edema in mice induced by either arachidonic acid (AA ) or 12-O-tetradecanoylphorbol acetate (TPA). The inhibition of the myeloperoxidase (MPO), marker enzyme of the accumulation of neutrophils in the inflamed tissue, was also investigated by the TPA model. The topical application of the olive oil compounds (0.5 mg/ear) produced a variable degree of antiinflammatory effect with both assays. In the auricular edema induced by TPA, β-sitosterol and erythrodiol from the unsaponifiable fraction of the oil showed a potent antiedematous effect with a 61.4% and 82.1% of inhibition respectively, values not very different to that of the reference indomethacin (85.6%) at 0.5 mg/ear. The four phenolics exerted a similar range of inhibition (33- 45%). All compounds strongly inhibited the enzyme myeloperoxidase, indicating a reduction of the neutrophil influx in the inflamed tissues. The strongest inhibitor of AA edema was the total unsaponifiable fraction which inhibition was 34%, similar to that obtained by the reference drug dexamethasone at 0.05 mg/ear. Among the phenolics, oleuropein also produced an inhibition of about 30% with the same dose, but all the other components were found less active in this assay. The anti-inflammatory effects exerted by both unsaponifiable and polar compounds might contribute to the potential biological properties reported for virgin olive oil against different pathological processess.

Fluorescent tetradecanoylphorbol acetate: A novel probe of phorbol ester binding domains

1991 ◽  
Vol 46 (3) ◽  
pp. 266-276 ◽  
Author(s):  
Margit Balázs ◽  
Jánòs Szöllösi ◽  
William C. Lee ◽  
Richard P. Haugland ◽  
Anthony P. Guzikowski ◽  
...  
Keyword(s):  
Phorbol Ester ◽  
Tetradecanoylphorbol Acetate ◽  
Binding Domains

scholarly journals Effects of increasing docosahexaenoic acid intake in human healthy volunteers on lymphocyte activation and monocyte apoptosis

2008 ◽  
Vol 101 (6) ◽  
pp. 852-858 ◽  
Author(s):  
Saïda Mebarek ◽  
Natalia Ermak ◽  
Amal Benzaria ◽  
Stéphanie Vicca ◽  
Madeleine Dubois ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Oxidized Ldl ◽  
Mrna Level ◽  
Lymphocyte Activation ◽  
Tetradecanoylphorbol Acetate ◽  
Apoptotic Effect ◽  
Monocyte Apoptosis ◽  
Induced Apoptosis ◽  
Dha Enrichment ◽  
Prevention Of Atherosclerosis

Dietary intake of long-chainn-3 PUFA has been reported to decrease several markers of lymphocyte activation and modulate monocyte susceptibility to apoptosis. However, most human studies examined the combined effect of DHA and EPA using relatively high daily amounts ofn-3 PUFA. The present study investigated the effects of increasing doses of DHA added to the regular diet of human healthy volunteers on lymphocyte response to tetradecanoylphorbol acetate plus ionomycin activation, and on monocyte apoptosis induced by oxidized LDL. Eight subjects were supplemented with increasing daily doses of DHA (200, 400, 800, 1600 mg) in a TAG form containing DHA as the only PUFA, for 2 weeks each dose. DHA intake dose-dependently increased the proportion of DHA in mononuclear cell phospholipids, the augmentation being significant after 400 mg DHA/d. The tetradecanoylphorbol acetate plus ionomycin-stimulated IL-2 mRNA level started to increase after ingestion of 400 mg DHA/d, with a maximum after 800 mg intake, and was positively correlated (P < 0·003) with DHA enrichment in cell phospholipids. The treatment of monocytes by oxidized LDL before DHA supplementation drastically reduced mitochondrial membrane potential as compared with native LDL treatment. Oxidized LDL apoptotic effect was significantly attenuated after 400 mg DHA/d and the protective effect was maintained throughout the experiment, although to a lesser extent at higher doses. The present results show that supplementation of the human diet with low DHA dosages improves lymphocyte activability. It also increases monocyte resistance to oxidized LDL-induced apoptosis, which may be beneficial in the prevention of atherosclerosis.

scholarly journals Prothymosin alpha gene expression correlates with proliferation, not differentiation, of HL-60 cells

Blood ◽  
1993 ◽  
Vol 82 (4) ◽  
pp. 1127-1132
Author(s):  
MR Smith ◽  
A al-Katib ◽  
R Mohammad ◽  
A Silverman ◽  
P Szabo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Myeloid Leukemia ◽  
Leukemia Cells ◽  
Monocytic Differentiation ◽  
Bryostatin 1 ◽  
Prothymosin Alpha ◽  
Tetradecanoylphorbol Acetate ◽  
Proliferation And Differentiation ◽  
Steady State Levels ◽  
Alpha Gene

Accumulating evidence suggests that prothymosin alpha has an as yet undefined intracellular, perhaps intranuclear, function related to cell proliferation. Prothymosin alpha mRNA and/or peptide levels increase when cells are stimulated to proliferate. Because proliferation and differentiation events are often inversely correlated, we examined prothymosin alpha gene expression during proliferation and differentiation of HL-60 myeloid leukemia cells. Steady-state levels of prothymosin alpha mRNA, which are high in exponentially growing HL-60, decrease within hours after induction of HL-60 to differentiate along the neutrophil pathway with dimethylsulfoxide (DMSO) or along the macrophage lineage with either tetradecanoylphorbol acetate (TPA) or bryostatin 1. The decline in prothymosin alpha mRNA in response to these differentiation signals parallels that of c-myc mRNA under the same conditions. We then determined whether the downregulation of prothymosin alpha and c-myc mRNA were due to differentiation or cessation or proliferation. Recombinant human gamma-interferon induces monocytic differentiation of HL-60, but permits continued proliferation, and, under these conditions, expression of prothymosin alpha, as well as of c-myc, mRNA remains elevated. We conclude that prothymosin alpha and c-myc expression are coregulated in differentiating HL-60 and that their expression correlates with the proliferative state of HL-60 cells, rather than with the differentiated state.

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