scholarly journals Chlorogenic Acid Improved High Glucose Induced Apoptosis and Osteogenic Differentiation in MC3T3-E1 Cells through Eliminating Oxidative Stress

2021 ◽  
Vol 77 (6) ◽  
pp. 881-887
Author(s):  
tian xu ◽  
Jizheng Zhang ◽  
Qiang Guo ◽  
Guangyu Wang ◽  
He Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chlorogenic Acid ◽  
Osteogenic Differentiation ◽  
High Glucose ◽  
Induced Apoptosis

α-Lipoic acid protects HAECs from high glucose-induced apoptosis via decreased oxidative stress, ER stress and mitochondrial injury

RSC Advances ◽  
2015 ◽  
Vol 5 (87) ◽  
pp. 70726-70736
Author(s):  
Wenshuang Li ◽  
Changyuan Wang ◽  
Jinyong Peng ◽  
Jing Liang ◽  
Yue Jin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Er Stress ◽  
Lipoic Acid ◽  
High Glucose ◽  
Vascular Diseases ◽  
Mitochondrial Injury ◽  
Wide Range ◽  
Induced Apoptosis

α-Lipoic acid (LA) has a wide range of benefits in treating diabetes mellitus (DM) and DM vascular diseases, however, the specific mechanisms are not clearly understood.

Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells

2018 ◽  
Vol 27 (10) ◽  
pp. 2609-2620 ◽  
Author(s):  
Ming-Bo Guo ◽  
De-Chun Wang ◽  
Hai-Fei Liu ◽  
Long-Wei Chen ◽  
Jian-Wei Wei ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nucleus Pulposus ◽  
High Glucose ◽  
Nucleus Pulposus Cells ◽  
Induced Apoptosis

Circ_0000491 Promotes Apoptosis, Inflammation, Oxidative Stress, and Fibrosis in High Glucose-Induced Mesangial Cells by Regulating miR-455-3p/Hmgb1 Axis

Nephron ◽  
2021 ◽  
pp. 1-12
Author(s):  
Jing Wang ◽  
Shifeng Yang ◽  
Wendong Li ◽  
Ming Zhao ◽  
Kai Li
Keyword(s):  
Oxidative Stress ◽  
Mouse Model ◽  
High Glucose ◽  
Mesangial Cells ◽  
Stress Factors ◽  
Luciferase Reporter ◽  
Circular Rnas ◽  
Enzyme Linked Immunosorbent Assay ◽  
Induced Apoptosis ◽  
Related Proteins

<b><i>Background:</i></b> Diabetic nephropathy (DN) is a severe microvascular complication of diabetes. Recently, many circular RNAs can exert crucial roles in DN progression. This study intended to explore the role and mechanism of circ_0000491 in DN. <b><i>Methods:</i></b> The DN mouse model was constructed by streptozotocin injection, and the DN cell model was established using high glucose (HG) treatment in mouse mesangial cells (SV40-MES13). The expression of circ_0000491 and microRNA-455-3p (miR-455-3p) was detected by quantitative real-time polymerase chain reaction. Cell apoptosis was evaluated by flow cytometry. The expression levels of high-mobility group box 1 (Hmgb1) protein, apoptosis-related proteins, and fibrosis-related proteins were examined by the Western blot assay. The release of inflammatory cytokines was assessed by enzyme-linked immunosorbent assay. The oxidative stress factors were analyzed by corresponding kits. The predicted interaction between miR-455-3p and circ_0000491 or Hmgb1 was verified by dual-luciferase reporter assay and RNA immunoprecipitation assay. <b><i>Results:</i></b> Circ_0000491 was overexpressed in the DN mouse model and HG-induced SV40-MES13 cells. Knockdown of circ_0000491 weakened HG-induced apoptosis, inflammation, oxidative stress, and fibrosis in SV40-MES13 cells. miR-455-3p was a direct target of circ_0000491, and miR-455-3p inhibition could reverse the role of circ_0000491 silencing in HG-induced SV40-MES13 cells. Moreover, Hmgb1 was a target gene of miR-455-3p, and miR-455-3p played a protective role against HG-induced cell injury by targeting Hmgb1. In addition, circ_0000491 regulated Hmgb1 expression by sponging miR-455-3p. <b><i>Conclusion:</i></b> Circ_0000491 knockdown inhibited HG-induced apoptosis, inflammation, oxidative stress, and fibrosis in SV40-MES13 cells by regulating miR-455-3p/Hmgb1 axis.

Sign in / Sign up

Export Citation Format

Share Document