scholarly journals DEVELOPMENT OF HYDROGEL CAPSULES FOR USE IN DUAL-PHASE TOPICAL PREPARATIONS CONTAINING DIHYDROXYACETONE AND AMINO ACIDS – PRELIMINARY RESULTS

2019 ◽  
Vol 76 (4) ◽  
pp. 715-724
Author(s):  
Bianka Jacyna ◽  
Barbara Mikolaszek ◽  
Bartosz Maciejewski ◽  
Krzysztof Cal
Keyword(s):  
Amino Acids ◽  
Dual Phase ◽  
Preliminary Results ◽  
Hydrogel Capsules

Polyglucose and Amino Acids: Preliminary Results

1997 ◽  
Vol 17 (2_suppl) ◽  
pp. 63-67 ◽  
Author(s):  
Bernadette Faller ◽  
Ty Shockley ◽  
Sandrine Genestier ◽  
Leo Martis
Keyword(s):  
Amino Acids ◽  
Preliminary Results

A Note on the Free Amino Acids in Some Small Species of Pogonophora

1980 ◽  
Vol 60 (1) ◽  
pp. 171-174 ◽  
Author(s):  
A. J. Southward ◽  
D. R. Dixon
Keyword(s):  
Amino Acids ◽  
Organic Compounds ◽  
Free Amino Acids ◽  
Free Amino ◽  
Whole Body ◽  
Small Species ◽  
Preliminary Results ◽  
Lack Of Information ◽  
Dissolved Organic Compounds

Previous studies on the significance of dissolved organic compounds in the nutrition of the smaller species of the phylum Pogonophora (Little & Gupta, 1968, 1969; Southward & Southward, 1968, 1970) were handicapped by lack of information about the levels of free amino acids in the tissues. In this brief note we give preliminary results for four species, using a modification of the technique described by Clark (1964, 1968, 1973) to estimate the amino acids in extracts of the whole body.

scholarly journals Aspects of the epidemiology of bovine tuberculosis in badgers and cattle. II. The development and use of a typing system for Mycobacterium bovis

1981 ◽  
Vol 86 (3) ◽  
pp. 247-257 ◽  
Author(s):  
P. A. Barrow
Keyword(s):  
Amino Acids ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Wild Animals ◽  
Simple Method ◽  
Preliminary Results ◽  
Source Of Infection ◽  
Typing System ◽  
Specific Variation

SUMMARYA relatively simple method for the detection of intra-specific variation of Mycobacterium bovis is described, based on the ability of strains to utilize or remove amino acids from solution. Using the method, 31 strains of M. bovis, isolated from badgers and cattle, have been divided into four major groups with some additional sub-groups and intermediate strains. Preliminary results suggest that the biotypes observed are relatively stable. The method was used in the investigation of an outbreak of bovine tuberculosis in cattle in Gloucestershire where badgers were implicated as a source of infection. The results suggested that transmission of infection had occurred on at least two or three separate occasions. It is suggested that the method could be used in studying the epidemiology of bovine tuberculosis in wild animals, cattle and man.

Free-zone electrophoresis in glass capillaries.

1981 ◽  
Vol 27 (9) ◽  
pp. 1551-1553 ◽  
Author(s):  
J W Jorgenson ◽  
K D Lukacs
Keyword(s):  
Amino Acids ◽  
Applied Voltage ◽  
Internal Diameter ◽  
Fluorescence Detector ◽  
Preliminary Results ◽  
Free Zone ◽  
Glass Capillaries ◽  
Zone Electrophoresis ◽  
Further Development ◽  
Fluorescent Derivatives

Abstract A simple theory of zone electrophoresis in open-tubular capillaries is developed. According to this theory, to achieve the highest resolution of zones, tubes with as small an inside diameter as possible should be used in combination with as high an applied voltage as feasible. To test this approach, we performed electrophoresis in glass capillaries with an internal diameter of 75 micron and a length of 100 cm. A special fluorescence detector was used to detect fluorescent zones while they migrated inside the capillary. With the application of 30 kV potentials to this system, rapid and efficient separations of amino acids, peptides, and urinary amines were demonstrated. In all cases fluorescent derivatives were necessary for detection. Preliminary results are encouraging, and with further development of sensitive detection devices, applications in clinical analyses may be feasible.

MO1011IDENTIFICATION OF EARLY BIOMARKERS OF PERITONEAL MEMBRANE DYSFUNCTION IN CHILDREN ON PERITONEAL DIALYSIS USING METABOLOMICS ANALYSIS -PRELIMINARY RESULTS OF AN ONGOING PROSPECTIVE STUDY

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Antonia Kondou ◽  
John Dotis ◽  
Vasiliki Karava ◽  
Olga Begou ◽  
Anna Taparkou ◽  
...  
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Peritoneal Dialysis ◽  
Prospective Study ◽  
Creatinine Clearance ◽  
Peritoneal Fluid ◽  
Statistical Difference ◽  
Peritoneal Membrane ◽  
Preliminary Results ◽  
Dialysis Duration

Abstract Background and Aims Peritoneal dialysis (PD) are the main renal replacement treatment for children and adolescents with end stage kidney disease (ESRD). Peritoneal fibrosis is a major complication in long-term PD patients. Aim of the present study is to record the metabolic "fingerprint" of children on PD and to investigate its correlation with PD history and dialysis adequacy as well as the emergence of potential biomarkers that could detect early or predict peritoneal dysfunction. Method Samples of serum, urine and peritoneal effluent collected from 15 children with ESRD on PD. At the same time and 6 months later was performed PET-test as well as assessment of KT/V and creatinine clearance. Samples were subjected to targeted metabolomic analysis of amino acids and derivatives. Regarding metabolic technologies used, all samples (peritoneal fluid, urine, blood) were analyzed by a hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS / MS) method previously developed and validated in our laboratory for the simultaneous determination of amino acids and their derivatives in biological fluids. Αalso, high flow analysis was carried out (LC-q-tof analysis – HPLC /MS ). Results Using (HILIC-MS / MS) method, we found out that peritoneal dialysis duration, presence or absence of diuresis and PD creatinine clearance values are associated with significant differences in the levels of several metabolites, including glycine, creatinine, proline and 4-hydroxyproline, leucine, valine, glutamine and glutamic acid. Using (LC-q-tof analysis – HPLC /MS) approximately 200 metabolites were analyzed in the aforementioned samples. Figure 1 illustrates the number of metabolites detected in each matrix, as well as the common metabolites between the three matrices. These metabolites were associated with peritoneal dialysis duration, creatine clearance and presence or absence of diuresis. Several metabolites showed statistical difference between the examined groups. In detail, regarding serum analysis, five metabolites, including hydroxy phenyl acetic acid, glutathione ox, glucosamine-6-P, indole acetic acid and riboflavin showed statistical difference between the examined groups based on PD vintage. Based on urine excretion four metabolites named histidine, shikimic acid, thiamine and methionine were statistically different. Concerning urine analysis, two metabolites namely uridine and itaconic acid showed statistical difference when patients sub grouped based on PD vintage. Peritoneal fluid analysis highlighted one metabolite, uridine, that levels are significantly lower in patients on PD therapy for more than 4 years while based on creatinine clearance levels subgroups, two metabolites, lactate and pantothenic acid present statistically significant difference. Conclusion Metabolomics may be a tool in the evaluation of patients with ESRD on PD as it appears to reflect the clinical phenotype of the patient and the functional phenotype of the peritoneal membrane. Our results are the preliminary results of an ongoing prospective study. Limitation of the study is the small sample of patients, which does not allow safe clinical interpretation.

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