Methylation level of the regulatory regions of the estrogen receptor type 1 gene in paravertebral muscles of girls with idiopathic scoliosis

2021 ◽  
Author(s):  
P Janusz ◽  
M Chmielewska ◽  
M Andrusiewicz ◽  
T Kotwicki ◽  
M Kotwicka
Keyword(s):  
Estrogen Receptor ◽  
Idiopathic Scoliosis ◽  
Methylation Level ◽  
Receptor Type ◽  
Regulatory Regions ◽  
Paravertebral Muscles

scholarly journals Methylation of estrogen receptor 2 (ESR2) in deep paravertebral muscles and its association with idiopathic scoliosis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Małgorzata Chmielewska ◽  
Piotr Janusz ◽  
Mirosław Andrusiewicz ◽  
Tomasz Kotwicki ◽  
Małgorzata Kotwicka
Keyword(s):  
Dna Methylation ◽  
Estrogen Receptor ◽  
Idiopathic Scoliosis ◽  
Cobb Angle ◽  
Methylation Level ◽  
Gene Promoter ◽  
Concave Side ◽  
Convex Side ◽  
Paravertebral Muscles ◽  
Moderate Negative Correlation

AbstractIdiopathic scoliosis (IS) is one of the most common spinal disorders in adolescents. Despite many studies, the etiopathogenesis of IS is still poorly understood. In recent years, the role of epigenetic factors in the etiopathogenesis of IS has been increasingly investigated. It has also been postulated that the development and progression of the disease is related to gender and puberty, and could be associated with estrogen action. Estrogen hormones act via estrogen receptor 1 (ESR1) and estrogen receptor 2 (ESR2). It has been suggested that ESR2 expression is dependent on methylation within its gene promoter. So far, no studies have evaluated local, tissue-specific DNA methylation in patients with IS. Thus, our study aimed to analyze the methylation and expression level of ESR2 in the paraspinal muscles of the convex and concave side of the IS curvature. The methylation level within ESR2 promoter 0N, but not exon 0N, was significantly higher on the concave side of the curvature compared to the convex side. There was no significant correlation between ESR2 expression and methylation level in the promoter 0N on the convexity of thoracic scoliosis, whereas, on the concave side of the curvature, we observed a moderate negative correlation. There was no difference in the methylation levels of the ESR2 promoter and exon 0N between groups of patients with Cobb angle ≤ 70° and > 70° on the concave and convex side of the curvature. We also found no statistically significant correlation between the Cobb angle value and the mean methylation level in either the ESR2 promoter or exon 0N on the convex or concave side of the curvature. Our findings demonstrate that DNA methylation at the ESR2 promoter in deep paravertebral muscle tissue is associated with the occurrence but not with the severity of idiopathic scoliosis.

scholarly journals Calcium-dependent energetics of calmodulin domain interactions with regulatory regions of the Ryanodine Receptor Type 1 (RyR1)

2014 ◽  
Vol 193-194 ◽  
pp. 35-49 ◽  
Author(s):  
Rhonda A. Newman ◽  
Brenda R. Sorensen ◽  
Adina M. Kilpatrick ◽  
Madeline A. Shea
Keyword(s):  
Ryanodine Receptor ◽  
Receptor Type ◽  
Regulatory Regions ◽  
Calcium Dependent ◽  
Domain Interactions

Dissecting the role of Corticotropin-releasing hormone receptor type 1 in Alzheimer's Disease

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
K Lerche ◽  
M Willem ◽  
K Kleinknecht ◽  
C Romberg ◽  
U Konietzko ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Hormone Receptor ◽  
Receptor Type ◽  
Corticotropin Releasing Hormone ◽  
Releasing Hormone
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