scholarly journals Validation of Ultrasensitive Mutant Huntingtin Detection in Human Cerebrospinal Fluid by Single Molecule Counting Immunoassay

2017 ◽  
Vol 6 (4) ◽  
pp. 349-361 ◽  
Author(s):  
Valentina Fodale ◽  
Roberto Boggio ◽  
Manuel Daldin ◽  
Cristina Cariulo ◽  
Maria Carolina Spiezia ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Single Molecule ◽  
Mutant Huntingtin ◽  
Human Cerebrospinal Fluid

scholarly journals Single molecule counting coupled to rapid amplification enables detection of αSynuclein aggregates in cerebrospinal fluid of PD patients

2021 ◽  
Author(s):  
Akshay BHUMKAR ◽  
Chloe MAGNAN ◽  
Derrick LAU ◽  
Eugene Soh Wei Jun ◽  
Nicolas DZAMKO ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Single Molecule ◽  
Rapid Amplification

scholarly journals Extracellular fluid, cerebrospinal fluid and plasma biomarkers of axonal and neuronal injury following intracerebral hemorrhage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lovisa Tobieson ◽  
Henrik Zetterberg ◽  
Kaj Blennow ◽  
Niklas Marklund
Keyword(s):  
Cerebrospinal Fluid ◽  
Intracerebral Hemorrhage ◽  
Brain Tissue ◽  
Single Molecule ◽  
Tissue Injury ◽  
Extracellular Fluid ◽  
Neurofilament Light ◽  
Aβ Peptides ◽  
Post Surgery ◽  
Fluid Compartments

AbstractSpontaneous intracerebral hemorrhage (ICH) is the most devastating form of stroke. To refine treatments, improved understanding of the secondary injury processes is needed. We compared energy metabolic, amyloid and neuroaxonal injury biomarkers in extracellular fluid (ECF) from the perihemorrhagic zone (PHZ) and non-injured (NCX) brain tissue, cerebrospinal fluid (CSF) and plasma. Patients (n = 11; age 61 ± 10 years) undergoing ICH surgery received two microdialysis (MD) catheters, one in PHZ, and one in NCX. ECF was analysed at three time intervals within the first 60 h post- surgery, as were CSF and plasma samples. Amyloid-beta (Aβ) 40 and 42, microtubule associated protein tau (tau), and neurofilament-light (NF-L) were analysed using Single molecule array (Simoa) technology. Median biomarker concentrations were lowest in plasma, higher in ECF and highest in CSF. Biomarker levels varied over time, with different dynamics in the three fluid compartments. In the PHZ, ECF levels of Aβ40 were lower, and tau higher when compared to the NCX. Altered levels of Aβ peptides, NF-L and tau may reflect brain tissue injury following ICH surgery. However, the dynamics of biomarker levels in the different fluid compartments should be considered in the study of pathophysiology or biomarkers in ICH patients.

scholarly journals Apolipoprotein E allelic influence on human cerebrospinal fluid apolipoproteins

1998 ◽  
Vol 39 (12) ◽  
pp. 2443-2451
Author(s):  
Kathleen S. Montine ◽  
Casey N. Bassett ◽  
Joyce J. Ou ◽  
William R. Markesbery ◽  
Larry L. Swift ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Apolipoprotein E ◽  
Human Cerebrospinal Fluid
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