Optimizing extraction of berberine and antioxidant compounds from barberry by maceration and pulsed electric field-assisted methods

2020 ◽  
pp. 1-16 ◽  
Author(s):  
Mozhdeh Sarraf ◽  
Adel Beig-babaei ◽  
Sara Naji-Tabasi
Keyword(s):  
Antioxidant Activity ◽  
Optimum Condition ◽  
Electric Field ◽  
Ethanol Concentration ◽  
Pulsed Electric Field ◽  
Antioxidant Compounds ◽  
Good Source ◽  
Valuable Compounds ◽  
Central Composite ◽  
Different Parts

BACKGROUND: Barberry is a valuable plant, which is useful in the treatment of different diseases. There are valuable compounds in different parts of barberry that are pruned per year and discarded as waste. OBJECTIVE: In this study, the amount of berberine, phenolic compounds and antioxidant activity of barberry fruits, leaves, and stems of different barberry species (B.integerrima and B.thunbergii) were investigated. METHODS: Central composite design of response surface methodology (RSM) were used in this study to investigate the extraction variables (time: 2–24 h, temperature: 24–70°C and ethanol concentration: 50–90%) in the extraction of berberine from the stem of B.integrrima. The responses used as criteria were the amount of berberine, scavenging radical DPPH, and polyphenol. Also, pulsed electric field-assisted (PEF) was applied as a pretreatment (Pulse strengths of 250, 1000 and 1250 Vcm–1; Pulse numbers (n) of 50 and 100; frequency (f) of 1 Hz) and then the extraction was conducted in optimum condition. PEF increased significantly the amount of berberine. RESULTS: The stem of B.integrrima had the most amounts of antioxidant activity and berberine content, and it was selected for more investigation. The optimum condition in the maceration method was: 90% of ethanol, 70°C, and 3.36 h (141.6 min). According to the result of PEF method, the best condition for extraction of berberine from barberry stems was 1000 Vcm–1 and n = 100. CONCLUSIONS: The results demonstrated that the extract of B.integrrima stem is a good source of berberine, antioxidant, and has the potential to be applied in various industries.

scholarly journals Quality stability and sensory attributes of apple juice processed by thermosonication, pulsed electric field and thermal processing

2016 ◽  
Vol 23 (3) ◽  
pp. 265-276 ◽  
Author(s):  
Alifdalino Sulaiman ◽  
Mohammed Farid ◽  
Filipa VM Silva
Keyword(s):  
Antioxidant Activity ◽  
Electric Field ◽  
Sensory Analysis ◽  
Thermal Processing ◽  
Apple Juice ◽  
Treatment Time ◽  
Pulsed Electric Field ◽  
Color Difference ◽  
Soluble Solids ◽  
Total Color Difference

Worldwide, apple juice is the second most popular juice, after orange juice. It is susceptible to enzymatic browning spoilage by polyphenoloxidase, an endogenous enzyme. In this study, Royal Gala apple juice was treated by thermosonication (TS: 1.3 W/mL, 58 ℃, 10 min), pulsed electric field (PEF: 24.8 kV/cm, 60 pulses, 169 µs treatment time, 53.8 ℃) and heat (75 ℃, 20 min) and stored at 3.0 ℃ and 20.0 ℃ for 30 days. A sensory analysis was carried out after processing. The polyphenoloxidase activity, antioxidant activity and total color difference of the apple juice were determined before and after processing and during storage. The sensory analysis revealed that thermosonication and pulsed electric field juices tasted differently from the thermally treated juice. Apart from the pulsed electric field apple juice stored at room temperature, the processed juice was stable during storage, since the pH and soluble solids remained constant and fermentation was not observed. Polyphenoloxidase did not reactivate during storage. Along storage, the juices’ antioxidant activity decreased and total color difference increased (up to 6.8). While the antioxidant activity increased from 86 to 103% with thermosonication and was retained after pulsed electric field, thermal processing reduced it to 67%. The processing increased the total color difference slightly. No differences in the total color difference of the juices processed by the three methods were registered after storage. Thermosonication and pulsed electric field could possibly be a better alternative to thermal preservation of apple juice, but refrigerated storage is recommended for pulsed electric field apple juice.

Extraction of Flavonoids from Clovers

2012 ◽  
Vol 195-196 ◽  
pp. 360-363
Author(s):  
Chun Gang Chen ◽  
Fen Xia Han ◽  
Yuan Zhang ◽  
Yu Zhong Shi
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Central Composite Design ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Response Surfaces ◽  
Second Order ◽  
Order Model ◽  
Solid Ratio ◽  
Central Composite

The extraction of flavonoids from clovers was optimized to maximize flavonoid yield Y in this study. A central composite design of response surface methodology involving extracting time, liquid-solid ratio, extracting temperature and ethanol concentration was used, and second-order model for Y was employed to generate the response surfaces. The optimum condition for Y was determined as follows: extracting time 24min, liquid-solid ratio 20, extracting temperature 80°C, and ethanol concentration 72%. Under the optimum condition, the flavonoid yield was 2.49%.

scholarly journals Optimization The Effect of Roasting Conditions by Central Composite Design (CCD) Method on The Antioxidant Compounds of Opuntia Ficus Indica Seeds From Morocco

2020 ◽  
Author(s):  
Chakir El Guezzane ◽  
Hamza El Moudden ◽  
Hicham Harhar ◽  
Abdelkader Zarrouk ◽  
Mohamed Tabyaoui
Keyword(s):  
Antioxidant Activity ◽  
Central Composite Design ◽  
Principal Component ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Opuntia Ficus Indica ◽  
Optimal Value ◽  
Total Flavonoids Content ◽  
The One ◽  
Central Composite

Abstract The effect of roasting conditions on antioxidant activity Opuntia Ficus Indica seeds from Morocco and their phenolic compounds were optimized by the Central Composite Design (CDD) method. The CCD was used to optimize the roasting conditions: temperature (X1: 60–200 °C) and roasting time (X2: 10–50 min). The best roasting conditions were used in order to optimize the optimal value of the response : TPC (Total Phenolic Content): X1: 200°C, X2: 50min with 104.86±1.94GAE/g extract predicted response, TFC (Total Flavonoids Content) : X1: 200 °C, X2: 50 min with 81.23±0.90 mg QE/g extract predicted responses, TTC (Total Condensed Tannins Content): X1: 128.9°C, X2: 34.92min with 6.12±0.95 mg QAE/g extract) predicted response. Moreover, the optimal potential antioxidant activity by ABTS assay and DPPH assay was found in extracts of Opuntia Ficus Indica seeds roasted: at a high temperature of the roasting. Furthermore, the positive significant correlations were determined by the principal component analysis (PCA) on the one hand, between the antioxidant capacity of the extracts and their antioxidants compounds (TPC and TFC), and on the other hand, between the two assays ABTS and DPPH. Consequently, the results of this work indicated that roasted Opuntia Ficus Indica seeds from Morocco can be considered as an essential ingredient for human foods.

scholarly journals Industry-Friendly Hydroethanolic Extraction Protocols for Grateloupia turuturu UV-Shielding and Antioxidant Compounds

2020 ◽  
Vol 10 (15) ◽  
pp. 5304
Author(s):  
Rafael Félix ◽  
Ana M. Carmona ◽  
Carina Félix ◽  
Sara C. Novais ◽  
Marco F. L. Lemos
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Procedure ◽  
Extraction Methods ◽  
Antioxidant Compounds ◽  
Uv Absorbance ◽  
Grateloupia Turuturu ◽  
Solid Ratio ◽  
Uv Shielding ◽  
Cosmetic Industry

Grateloupia turuturu is an invasive macroalga on the Iberian coast, known to produce bioactive compounds with different cosmeceutical bioactivities, namely UV shielding and antioxidants. The goal of this study was to optimize the extraction procedure of main bioactivities of this species with cosmetic potential, using Response Surface Methodology. Two Box–Behnken designs were used to evaluate the effect of ethanol concentration (0–50%), liquid-solid ratio, time, pH, and temperature on yield, UV absorbance, and antioxidant activity. Both optimizations showed a similar trend: aqueous extracts have higher yields and extracts performed with ethanol as part of the solvent have higher activities concerning UV absorbance and antioxidant activity. For all the extracts an absorption peak between 320 and 340 nm was observed. This data now allows further studies by narrowing the extracts worthful of characterization. The development of industry-friendly extraction methods allows the valorization of this invasive species, contributing for the potential creation of natural and eco-friendly products by the cosmetic industry while contributing to the restoration of affected environments.

Antioxidant activity improvement of identified pine nut peptides by pulsed electric field (PEF) and the mechanism exploration

LWT ◽  
2017 ◽  
Vol 75 ◽  
pp. 366-372 ◽  
Author(s):  
Songyi Lin ◽  
Rong Liang ◽  
Peiyu Xue ◽  
Shuyu Zhang ◽  
Zeyu Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Electric Field ◽  
Pulsed Electric Field ◽  
Pine Nut
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