Inhibition of Tulane Virus replication via exposure to lowbush blueberry (Vaccinium angustifolium) fractional components

2017 ◽  
Vol 7 (4) ◽  
pp. 281-289 ◽  
Author(s):  
David F. Bridges ◽  
Anna Breard ◽  
Alison Lacombe ◽  
Don C. Valentine ◽  
Shravani Tadepalli ◽  
...  
Keyword(s):  
Virus Replication ◽  
Vaccinium Angustifolium ◽  
Lowbush Blueberry ◽  
Tulane Virus

INORGANIC NITROGEN LEVELS AND NITRIFICATION POTENTIAL IN LOWBUSH BLUEBERRY SOILS

1988 ◽  
Vol 68 (1) ◽  
pp. 63-75 ◽  
Author(s):  
LEONARD J. EATON ◽  
DAVID G. PATRIQUIN
Keyword(s):  
Ammonium Nitrate ◽  
Ammonium Sulfate ◽  
Soil Nitrogen ◽  
Plant Uptake ◽  
Inorganic Nitrogen ◽  
Garden Soil ◽  
Laboratory Studies ◽  
Vaccinium Angustifolium ◽  
Nitrogen Levels ◽  
Lowbush Blueberry

Soil ammonium and nitrate in the top 15 cm of soil were monitored after application of ammonium nitrate and ammonium sulfate to plots at 14 PF (previously fertilized) and 12 NF (never fertilized) lowbush blueberry (Vaccinium angustifolium Ait.) stands representing a range of soil types and management histories. Overall, nitrate values in unfertilized and ammonium sulfate plots were higher at PF than at NF sites, suggesting greater nitrification at PF sites. In laboratory incubation studies, nitrification proceeded immediately in soil from a PF site, but only after a 4-wk lag in that from an adjacent NF site. Nitrification rates were low compared to that in a garden soil (pH 6.6). N-Serve inhibited nitrification in both soils. In ammonium nitrate plots, "excess" N values (N values in fertilized plots minus values in unfertilized plots) were higher for PF than for NF sites, suggesting greater immobilization, plant uptake or loss of N at NF sites. There was no evidence, in laboratory studies, of immobilization of added N by soil from either type of site. Rhizome N concentration increased significantly in response to fertilization at an NF site, but not at a PF site. Key words: Blueberry (lowbush), fertilizer and soil nitrogen

Studies on the flowering biology of red sorrel (Rumex acetosella) ramets from lowbush blueberry (Vaccinium angustifolium) fields in Nova Scotia, Canada

Botany ◽  
2015 ◽  
Vol 93 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Scott N. White ◽  
Nathan S. Boyd ◽  
Rene C. Van Acker ◽  
Clarence J. Swanton
Keyword(s):  
Nova Scotia ◽  
Combined Effects ◽  
Perennial Species ◽  
Weed Species ◽  
Vaccinium Angustifolium ◽  
Perennial Weed ◽  
Flowering Response ◽  
Lowbush Blueberry ◽  
Rumex Acetosella

Red sorrel (Rumex acetosella L.) is a ramet-producing herbaceous creeping perennial species commonly found as a weed in commercially managed lowbush blueberry (Vaccinium angustifolium Aiton) fields in Nova Scotia, Canada. Flowering and seed production occur primarily in overwintering ramets of this species, indicating a potential vernalization requirement for flowering. This study was therefore initiated to examine the role of vernalization, photoperiod, and pre-vernalization stimulus on ramet flowering. Red sorrel ramets propagated from creeping roots and seeds collected from established red sorrel populations in lowbush blueberry had an obligate requirement for vernalization to flower. Ramet populations maintained under pre- and post-vernalization photoperiods of 16 h flowered following 12 weeks of vernalization at 4 ± 0.1 °C, whereas those maintained under constant 16, 14, or 8 h photoperiods without vernalization did not flower. Vernalization for 10 weeks maximized, but did not saturate, the flowering response. Pre-vernalization photoperiod affected flowering response, with increased flowering frequency observed in ramet populations exposed to decreasing, rather than constant, photoperiod prior to vernalization. This study represents the first attempt to determine the combined effects of vernalization and photoperiod on red sorrel flowering, and the results provide a benchmark for the future study of flowering and sexual reproduction in this economically important perennial weed species.

Response of lowbush blueberry (Vaccinium angustifolium) to hexazinone applied early in the fruiting year

2002 ◽  
Vol 82 (4) ◽  
pp. 781-783 ◽  
Author(s):  
K. I. N. Jensen ◽  
E. G. Specht
Keyword(s):  
Key Words ◽  
Weed Control ◽  
Corolla Tube ◽  
Growth Stages ◽  
Vaccinium Angustifolium ◽  
Floral Buds ◽  
Perennial Weeds ◽  
Lowbush Blueberry ◽  
Herbaceous Perennial

Spring application of 1.0 kg ha-1 hexazinone to fruiting-year lowbush blueberry no later than the F3 floral stage, when floral buds separate, but before the corolla tube shows white, controlled some common herbaceous perennial weeds without injury to the crop. Key words: Herbicide injury, growth stages, weed control, hexazinone, Vaccinium angustifolium

Long-term effects of herbicide and fertilizers on lowbush blueberry growth and production

1994 ◽  
Vol 74 (2) ◽  
pp. 341-345 ◽  
Author(s):  
Leonard J. Eaton
Keyword(s):  
Reproductive Development ◽  
Fresh Fruit ◽  
Long Term Effects ◽  
Vaccinium Angustifolium ◽  
Nitrogen Levels ◽  
Lowbush Blueberry ◽  
Production Cycles ◽  
Npk Fertilizer ◽  
Vegetative And Reproductive Growth

Effects of herbicide and herbicide-fertilizer combinations on vegetative and reproductive growth of the lowbush blueberry (Vaccinium angustifolium Ait.) were assessed over a 12-yr period (six production cycles). All treatments stimulated stem lengths, fruit buds per stem and fresh fruit yields compared with untreated controls, but only after 3–6 yr. After the second cycle, stem lengths and fruit bud numbers were greater in herbicide + NPK-treated plants than in all others. Fresh fruit yields were variable throughout the study but were higher in herbicide and herbicide + fertilizer plots than in untreated controls after 1982, except in 1988. Nitrogen levels were greater in leaves and rhizomes of plants in all herbicide and herbicide + fertilizer plots than in control plots after 10 yr, whereas phosphorus was higher only in plants fertilized with NPK. These results suggest the lowbush blueberry responds slowly to herbicide and fertilizer applications. Increased vegetative and reproductive development, as well as denser plant stands, resulted from repeated herbicide and fertilizer applications. Fertilizers applied in conjunction with herbicides stimulate vegetative growth (and fruit buds if an NPK fertilizer), but appear to have no consistent effect on fresh fruit yields. Key words:Vaccinium angustifolium, fertilizer, herbicide, fruit buds, yield

Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

2018 ◽  
Vol 32 (4) ◽  
pp. 705-714 ◽  
Author(s):  
Christina Ly ◽  
Jonathan Ferrier ◽  
Jeremiah Gaudet ◽  
Julien Yockell-Lelièvre ◽  
John Thor Arnason ◽  
...  
Keyword(s):  
Cell Migration ◽  
Leaf Extract ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Migration And Invasion ◽  
Vaccinium Angustifolium ◽  
Cell Migration And Invasion ◽  
Lowbush Blueberry

scholarly journals Recovirus NS1-2 Has Viroporin Activity That Induces Aberrant Cellular Calcium Signaling To Facilitate Virus Replication

mSphere ◽  
2019 ◽  
Vol 4 (5) ◽  
Author(s):  
Alicia C. Strtak ◽  
Jacob L. Perry ◽  
Mark N. Sharp ◽  
Alexandra L. Chang-Graham ◽  
Tibor Farkas ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Calcium Signaling ◽  
Virus Replication ◽  
Acute Gastroenteritis ◽  
Diarrheal Disease ◽  
Enteric Viruses ◽  
Functional Assay ◽  
Human Noroviruses ◽  
Tulane Virus ◽  
Enteric Caliciviruses

ABSTRACT Enteric viruses in the Caliciviridae family cause acute gastroenteritis in humans and animals, but the cellular processes needed for virus replication and disease remain unknown. A common strategy among enteric viruses, including rotaviruses and enteroviruses, is to encode a viral ion channel (i.e., viroporin) that is targeted to the endoplasmic reticulum (ER) and disrupts host calcium (Ca2+) homeostasis. Previous reports have demonstrated genetic and functional similarities between the nonstructural proteins of caliciviruses and enteroviruses, including the calicivirus NS1-2 protein and the 2B viroporin of enteroviruses. However, it is unknown whether caliciviruses alter Ca2+ homeostasis for virus replication or whether the NS1-2 protein has viroporin activity like its enterovirus counterpart. To address these questions, we used Tulane virus (TV), a rhesus enteric calicivirus, to examine Ca2+ signaling during infection and determine whether NS1-2 has viroporin activity that disrupts Ca2+ homeostasis. We found that TV increases Ca2+ signaling during infection and that increased cytoplasmic Ca2+ levels are important for efficient replication. Further, TV NS1-2 localizes to the endoplasmic reticulum, the predominant intracellular Ca2+ store, and the NS2 region has characteristics of a viroporin domain (VPD). NS1-2 had viroporin activity in a classic bacterial functional assay and caused aberrant Ca2+ signaling when expressed in mammalian cells, but truncation of the VPD abrogated these activities. Together, our data provide new mechanistic insights into the function of the NS2 region of NS1-2 and support the premise that enteric viruses, including those within Caliciviridae, exploit host Ca2+ signaling to facilitate their replication. IMPORTANCE Tulane virus is one of many enteric caliciviruses that cause acute gastroenteritis and diarrheal disease. Globally, enteric caliciviruses affect both humans and animals and amass >65 billion dollars per year in treatment and health care-associated costs, thus imposing an enormous economic burden. Recent progress has resulted in several cultivation systems (B cells, enteroids, and zebrafish larvae) to study human noroviruses, but mechanistic insights into the viral factors and host pathways important for enteric calicivirus replication and infection are still largely lacking. Here, we used Tulane virus, a calicivirus that is biologically similar to human noroviruses and can be cultivated by conventional cell culture, to identify and functionally validate NS1-2 as an enteric calicivirus viroporin. Viroporin-mediated calcium signaling may be a broadly utilized pathway for enteric virus replication, and its existence within caliciviruses provides a novel approach to developing antivirals and comprehensive therapeutics for enteric calicivirus diarrheal disease outbreaks.

Vaccinium angustifolium (Wild lowbush blueberry)

2019 ◽  
pp. 2641-2641
Author(s):  
K. Subramanya Sastry ◽  
Bikash Mandal ◽  
John Hammond ◽  
S. W. Scott ◽  
R. W. Briddon
Keyword(s):  
Vaccinium Angustifolium ◽  
Lowbush Blueberry

scholarly journals Poecilus lucublandus (Coleoptera: Carabidae) and Pterostichus mutus Do Not Feed on Hair Fescue, Red Sorrel, and Poverty Oatgrass Seeds

2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Alexandre M M C Loureiro ◽  
G Christopher Cutler ◽  
Vilis O Nams ◽  
Scott N White
Keyword(s):  
Seed Predation ◽  
Choice Test ◽  
Weed Seed ◽  
Weed Species ◽  
Vaccinium Angustifolium ◽  
Lowbush Blueberry ◽  
Rumex Acetosella ◽  
Weed Seeds ◽  
Poecilus Lucublandus ◽  
Danthonia Spicata

Abstract Poecilus lucublandus (Say), Pterostichus mutus (Say), and Harpalus rufipes (De Geer) are abundant Carabidae in lowbush blueberry fields and may contribute to weed seed predation. We used laboratory no-choice test experiments to determine if these beetles feed on seeds of hair fescue (Festuca filiformis Pourr., Poales: Poaceae), poverty oatgrass (Danthonia spicata L.), and red sorrel (Rumex acetosella L., Caryophyllales: Polygonaceae), which are common weeds in lowbush blueberry (Vaccinium angustifolium Ait., Ericales: Ericaceae) fields. Poecilus lucublandus and P. mutus did not feed on seeds of the test weed species, but H. rufipes consumed on average over 30 seeds of each species. There are other weed seeds in blueberry fields that could be palatable to P. lucublandus and P. mutus, which warrants further research on the granivory potential of these important carabid species.

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