A high-throughput marker-assisted selection system combining rapid DNA extraction high-resolution melting and simple sequence repeat analysis: Strawberry as a model for fruit crops

2017 ◽  
Vol 7 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Young-Hee Noh ◽  
Seonghee Lee ◽  
Vance M. Whitaker ◽  
Kelsey R. Cearley ◽  
Jae-Soon Cha
Keyword(s):  
High Resolution ◽  
Simple Sequence Repeat ◽  
Marker Assisted Selection ◽  
High Resolution Melting ◽  
Selection System ◽  
Sequence Repeat ◽  
Fruit Crops ◽  
Repeat Analysis ◽  
Simple Sequence Repeat Analysis ◽  
Simple Sequence

Genetic diversity among a founder parent and widely grown wheat cultivars derived from the same origin based on morphological traits and microsatellite markers

2012 ◽  
Vol 63 (4) ◽  
pp. 303 ◽  
Author(s):  
X. J. Li ◽  
X. Xu ◽  
X. M. Yang ◽  
X. Q. Li ◽  
W. H. Liu ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Simple Sequence Repeat ◽  
Morphological Traits ◽  
Sequence Repeat ◽  
Repeat Analysis ◽  
Simple Sequence Repeat Analysis ◽  
Close Proximity ◽  
Genome Information ◽  
Simple Sequence ◽  
Founder Parent

Founder parents have contributed significantly to the improvement of wheat. Beijing 8 has been used as a founder parent in developing many outstanding improved cultivars in China. The widely grown cultivars Beijing 8 and 6 additional derivatives both derived from the cross ‘Bima 4 × Early Premium’ in China, were characterised using seven morphological traits and 537 microsatellite markers. Phenotypic comparisons revealed that Beijing 8 was similar for certain characteristics to the widely grown cultivars Shijiazhuang 54 and Jinan 2, hinting that acceptable performance for yield components may be the basis for Beijing 8 serving as a founder parent. Simple sequence repeat analysis indicated that Bima 4 contributed more genome information to the derivatives than Early Premium. Fifty-nine unique simple sequence repeat alleles, present in Beijing 8 and absent in other cultivars, were observed. Nearly all loci were in close proximity to the positions of known genes conferring important traits. Furthermore, pedigree tracking found that the frequencies of alleles unique to Beijing 8 varied from 0 to 0.96 in its 51 descendants, suggesting that some of them underwent rigorous selection during breeding.

scholarly journals Genetic variations and dog breed identification using inter-simple sequence repeat markers coupled with high resolution melting analysis

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10215
Author(s):  
Wannapimol Kriangwanich ◽  
Korakot Nganvongpanit ◽  
Kittisak Buddhachat ◽  
Puntita Siengdee ◽  
Siriwadee Chomdej ◽  
...  
Keyword(s):  
High Resolution ◽  
Simple Sequence Repeat ◽  
High Resolution Melting ◽  
Inter Simple Sequence Repeat ◽  
Genetic Variations ◽  
High Resolution Melting Analysis ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Melting Analysis ◽  
Simple Sequence

The identification of differing physical characteristics of dogs is an uncomplicated and straightforward way to categorize dog breeds. However, many dog owners and veterinarians still struggle to distinguish between pure breed and mixed variations in certain breeds of dogs. Presently, the absence of the tools and methods needed to confirm a pure breed dog is a significant problem since the only method available to validate pure or mongrel breeds is the official pedigree system. Inter-simple sequence repeat markers have been successfully used to assess genetic variations and differentiations. Notably, inter-simple sequence repeat markers coupled with high resolution melting analysis were effectively used for the breed identification of 43 breeds of dogs (total 463 dogs). The 10 primers chosen for analysis resulted in a range of 31–78.6% of breed discrimination when using one primer, while a combination of two primers was able to successfully discriminate between all of the 43 dog breeds (100%). Shannon’s index information (I = 2.586 ± 0.034) and expected heterozygosity (He = 0.908 ± 0.003) indicated a high level of genetic diversity among breeds. The fixation index (Fst) revealed a value of 10.4%, demonstrating that there was a high level of genetic subdivision between populations. This study showed that inter-simple sequence repeat marker analysis was effective in demonstrating high genetic diversity among varying breeds of dogs, while a combination of Inter-simple sequence repeat marker analysis and high resolution melting analysis could provide an optional technique for researchers to effectively identify breeds through genetic variations.

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