Electric field and electric potential due to a finite cylindrical surface charge distribution considering a linearly variable surface charge density

2016 ◽  
Vol 51 (4) ◽  
pp. 471-480
Author(s):  
Guillermo A. Díaz ◽  
Enrique E. Mombello ◽  
Geovanny A. Marulanda
Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.


2021 ◽  
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe

Abstract Background: Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. Results: This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle interactions. Nanoparticles with fluorescence were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescence light intensity and surface charge density, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, HUVECs and Hela cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potential of the two types of cells was obtained, which is in good agreement with the standard electrophoretic light scattering measurement. Conclusions: This method can be used for rapid surface charge mapping of single particles or cells and can advance cell-surface-charge characterization applications in many biomedical fields.


Nanoscale ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 4538-4544 ◽  
Author(s):  
Thomas Fuhs ◽  
Lasse Hyldgaard Klausen ◽  
Steffan Møller Sønderskov ◽  
Xiaojun Han ◽  
Mingdong Dong

The local surface charge density of the cell membrane influences regulation and localization of membrane proteins.


Author(s):  
Andrey B. Yakovlev ◽  
Valeriya S. Federyaeva

AbstractAn analytical solution is presented for the electric field response generated by a nonconducting ellipsoid (prolate spheroid) in a homogeneous conducting fluid subject to an external primary electric field, including surface charge distribution. Such a solution might be useful for different purposes, including cell modeling subject to an external quasistatic electromagnetic stimulus. The solution utilizes the well-known analogy between the electrostatics of dielectrics and DC conduction. The solution obtained includes an expression for the volumetric fields and an expression for the induced surface charge density at the membrane.


Micron ◽  
2017 ◽  
Vol 100 ◽  
pp. 45-49 ◽  
Author(s):  
Diganta Dutta ◽  
Xavier-Lewis Palmer ◽  
Anthony Asmar ◽  
Michael Stacey ◽  
Shizhi Qian

2007 ◽  
Vol 17 (06) ◽  
pp. 845-876 ◽  
Author(s):  
PATRICK CIARLET ◽  
SAMIR KADDOURI

We are interested in computing the charge density and the electric field at the rounded tip of an electrode of small curvature. As a model, we focus on solving the electrostatic problem for the electric potential. For this problem, Peek's empirical formulas describe the relation between the electric field at the surface of the electrode and its curvature radius. However, it can be used only for electrodes with either a purely cylindrical, or a purely spherical, geometrical shape. Our aim is to justify rigorously these formulas, and to extend it to more general, two-dimensional, or three-dimensional axisymmetric, geometries. With the help of multiscaled asymptotic expansions, we establish an explicit formula for the electric potential in geometries that coincide with a cone at infinity. We also prove a formula for the surface charge density, which is very simple to compute with the Finite Element Method. In particular, the meshsize can be chosen independently of the curvature radius. We illustrate our mathematical results by numerical experiments.


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