Characterization of the F198S prion protein mutation: Enhanced glycosylation and defective refolding

2005 ◽  
Vol 7 (2) ◽  
pp. 159-171 ◽  
Author(s):  
Syed I.A. Zaidi ◽  
Sandra L. Richardson ◽  
Sabina Capellari ◽  
Li Song ◽  
Mark A. Smith ◽  
...  
Keyword(s):  
Prion Protein ◽  
Protein Mutation

scholarly journals Characterization of four new monoclonal antibodies against the distal N-terminal region of PrPc

2014 ◽  
Author(s):  
Alessandro Didonna ◽  
Anja Colja Venturini ◽  
Katrina Hartman ◽  
Tanja Vranac ◽  
Vladka Curin Serbec ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Prion Protein ◽  
Biochemical Characterization ◽  
Prion Diseases ◽  
Physiological Role ◽  
Prion Infection ◽  
Promising Tool ◽  
C Terminus ◽  
N Terminus

Prion diseases are a group of fatal neurodegenerative disorders that affect humans and animals. They are characterized by the accumulation in the central nervous system of a pathological form of the host-encoded prion protein (PrPC). The prion protein is a membrane glycoprotein that consists of two domains: a globular, structured C-terminus and an unstructured N-terminus. The N-terminal part of the protein is involved in different functions in both health and disease. In the present work we discuss the production and biochemical characterization of a panel of four monoclonal antibodies (mAbs) against the distal N-terminus of PrPC using a well-established methodology based on the immunization of Prnp0/0 mice. Additionally, we show their ability to block prion (PrPSc) replication at nanomolar concentrations in a cell culture model of prion infection. These mAbs represent a promising tool for prion diagnostics and for studying the physiological role of the N-terminal domain of PrPC.

scholarly journals Characterization of Recombinant, Membrane-attached Full-length Prion Protein

2004 ◽  
Vol 279 (24) ◽  
pp. 25058-25065 ◽  
Author(s):  
Heike Eberl ◽  
Peter Tittmann ◽  
Rudi Glockshuber
Keyword(s):  
Prion Protein ◽  
Full Length

Neurovegetative control is altered in transgenic mice expressing the prion protein mutation associated with fatal familial insomnia

2015 ◽  
Vol 192 ◽  
pp. 107 ◽  
Author(s):  
M.A. Wu ◽  
E. Tobaldini ◽  
S. Bianchi ◽  
F. Del Gallo ◽  
I. Bouybayoune ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Prion Protein ◽  
Fatal Familial Insomnia ◽  
Protein Mutation

scholarly journals Characterization of Detergent-insoluble Complexes Containing the Cellular Prion Protein and Its Scrapie Isoform

1997 ◽  
Vol 272 (10) ◽  
pp. 6324-6331 ◽  
Author(s):  
Naava Naslavsky ◽  
Ronit Stein ◽  
Anat Yanai ◽  
Gilgi Friedlander ◽  
Albert Taraboulos
Keyword(s):  
Prion Protein ◽  
Cellular Prion Protein

Proteomics Approach to Identify the Interacting Partners of Cellular Prion Protein and Characterization of Rab7a Interaction in Neuronal Cells

2011 ◽  
Vol 10 (7) ◽  
pp. 3123-3135 ◽  
Author(s):  
Saima Zafar ◽  
Nicolas von Ahsen ◽  
Michael Oellerich ◽  
Inga Zerr ◽  
Walter J. Schulz-Schaeffer ◽  
...  
Keyword(s):  
Prion Protein ◽  
Neuronal Cells ◽  
Cellular Prion Protein ◽  
Proteomics Approach

scholarly journals Characterization of the Prion Protein in Human Urine

2010 ◽  
Vol 285 (40) ◽  
pp. 30489-30495 ◽  
Author(s):  
Ayuna Dagdanova ◽  
Serguei Ilchenko ◽  
Silvio Notari ◽  
Qiwei Yang ◽  
Mark E. Obrenovich ◽  
...  
Keyword(s):  
Prion Protein ◽  
Human Urine

scholarly journals Structural characterization of POM 6 Fab and mouse prion protein complex identifies key regions for prions conformational conversion

FEBS Journal ◽  
2018 ◽  
Vol 285 (9) ◽  
pp. 1701-1714 ◽  
Author(s):  
Pravas Kumar Baral ◽  
Mridula Swayampakula ◽  
Adriano Aguzzi ◽  
Michael N. G. James
Keyword(s):  
Prion Protein ◽  
Structural Characterization ◽  
Protein Complex ◽  
Conformational Conversion
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