Specific Uptake of an Amyloid-β Protofibril-Binding Antibody-Tracer in AβPP Transgenic Mouse Brain

2013 ◽  
Vol 37 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Kristina Magnusson ◽  
Dag Sehlin ◽  
Stina Syvänen ◽  
Marie M. Svedberg ◽  
Ola Philipson ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain ◽  
Amyloid Β ◽  
Specific Uptake ◽  
Binding Antibody

scholarly journals First effects of rising amyloid-β in transgenic mouse brain: synaptic transmission and gene expression

Brain ◽  
2015 ◽  
Vol 138 (7) ◽  
pp. 1992-2004 ◽  
Author(s):  
Damian M. Cummings ◽  
Wenfei Liu ◽  
Erik Portelius ◽  
Sevinç Bayram ◽  
Marina Yasvoina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Synaptic Transmission ◽  
Transgenic Mouse ◽  
Mouse Brain ◽  
Amyloid Β

scholarly journals 25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner

2020 ◽  
Author(s):  
Man Ying Wong ◽  
Michael Lewis ◽  
James J Doherty ◽  
Yang Shi ◽  
Anil G Cashikar ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Brain Tissue ◽  
Transgenic Mouse ◽  
Mouse Brain ◽  
Amyloid Β ◽  
Genome Wide Association Studies ◽  
Dependent Manner ◽  
Tau Pathology ◽  
Caspase 1 ◽  
Primary Mouse

Abstract Background : Genome-wide association studies of Alzheimer’s disease (AD) have implicated pathways related to lipid homeostasis and innate immunity in AD pathophysiology. However, the exact cellular and chemical mediators of neuroinflammation in AD remain poorly understood. The oxysterol 25-hydroxycholesterol (25-HC) is an important immunomodulator produced by peripheral macrophages with wide-ranging effects on cell signaling and innate immunity. Genetic variants of the enzyme responsible for 25-HC production, cholesterol 25‑hydroxylase (CH25H), have been found to be associated with AD. Methods: We used real time-PCR and immunoblotting to examine CH25H expression in human AD brain tissue and in transgenic mouse brain tissue bearing amyloid-β plaques or tau pathology. The innate immune response of primary mouse microglia under different treatment condition or bearing different genetic backgrounds was analyzed using ELISA, western blotting or immunocytochemistry. Results : We found that CH25H expression is upregulated in human AD brain tissue and in transgenic mouse brain tissue bearing amyloid-β plaques or tau pathology. Treatment with the toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS) markedly upregulates CH25H expression in the mouse brain and stimulates CH25H expression and 25-HC secretion in mouse primary microglia. We found that LPS-induced microglial production of the pro-inflammatory cytokine IL-1β is markedly potentiated by 25-HC and attenuated by deletion of CH25H. Microglia expressing apolipoprotein E4 (apoE4), a genetic risk factor for AD, produce greater amounts of 25-HC than apoE3-expressing microglia following treatment with LPS. Remarkably, 25-HC treatment results in a greater level of IL-1β secretion in LPS-activated apoE4-expressing microglia than in apoE2- or apoE3-expressing microglia. Blocking potassium efflux or inhibiting caspase-1 prevents 25-HC-potentiated IL-1β release in apoE4-expressing microglia, indicating the involvement of caspase-1/NLRP3 inflammasome activity. Conclusion : 25-HC may function as a microglial secreted inflammatory mediator in brain, promoting IL-1β-mediated neuroinflammation in an apoE isoform-dependent manner (E4>>E2/E3) and thus may be an important mediator of neuroinflammation in AD.

scholarly journals Coffee polyphenols prevent cognitive dysfunction and suppress amyloid β plaques in APP/PS2 transgenic mouse

2020 ◽  
Vol 154 ◽  
pp. 35-44 ◽  
Author(s):  
Keiko Ishida ◽  
Masaki Yamamoto ◽  
Kensuke Misawa ◽  
Hitomi Nishimura ◽  
Koichi Misawa ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Cognitive Dysfunction ◽  
Amyloid Β

Proteomic analysis of the brain tissues from a transgenic mouse model of amyloid β oligomers

2012 ◽  
Vol 61 (3) ◽  
pp. 347-355 ◽  
Author(s):  
Masaoki Takano ◽  
Kouji Maekura ◽  
Mieko Otani ◽  
Keiji Sano ◽  
Tooru Nakamura-Hirota ◽  
...  
Keyword(s):  
Mouse Model ◽  
Transgenic Mouse ◽  
Proteomic Analysis ◽  
Amyloid Β ◽  
Transgenic Mouse Model ◽  
The Brain ◽  
Brain Tissues

scholarly journals Subcellular Localization of Wild-Type and Parkinson's Disease-Associated Mutant α-Synuclein in Human and Transgenic Mouse Brain

2000 ◽  
Vol 20 (17) ◽  
pp. 6365-6373 ◽  
Author(s):  
Philipp J. Kahle ◽  
Manuela Neumann ◽  
Laurence Ozmen ◽  
Veronika Müller ◽  
Helmut Jacobsen ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Subcellular Localization ◽  
Transgenic Mouse ◽  
Mouse Brain ◽  
Wild Type

Age-Associated Inclusions in Normal and Transgenic Mouse Brain

Science ◽  
1992 ◽  
Vol 255 (5050) ◽  
pp. 1445-1445 ◽  
Author(s):  
D. WIRAK ◽  
R. BAYNEY ◽  
T. V. RAMABHADRAN ◽  
R. P. FRACASSO ◽  
J. T. HART ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain
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