scholarly journals MR Microscopy of Human Amyloid-β Deposits: Characterization of Parenchymal Amyloid, Diffuse Plaques, and Vascular Amyloid

2013 ◽  
Vol 34 (4) ◽  
pp. 1037-1049 ◽  
Author(s):  
Rob J.A. Nabuurs ◽  
Remco Natté ◽  
Fenna M. de Ronde ◽  
Ingrid Hegeman-Kleinn ◽  
Jouke Dijkstra ◽  
...  
Keyword(s):  
Amyloid Β ◽  
Mr Microscopy ◽  
Diffuse Plaques

Alzheimer's disease amyloid β-clipping enzyme (APP secretase): Identification, purification, and characterization of the enzyme

1991 ◽  
Vol 177 (1) ◽  
pp. 377-387 ◽  
Author(s):  
Kazuhiko Tagawa ◽  
Tatsuhide Kunishita ◽  
Kei Maruyama ◽  
Kazuaki Yoshikawa ◽  
Eiki Kominami ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Amyloid Β ◽  
Purification And Characterization

Genetic characterization of amyloid-β and tau network spread

2018 ◽  
Vol 24 (12) ◽  
pp. 1790-1792
Author(s):  
Rik Ossenkoppele ◽  
Oskar Hansson
Keyword(s):  
Genetic Characterization ◽  
Amyloid Β

scholarly journals Characterization of the unique In Vitro effects of unsaturated fatty acids on the formation of amyloid β fibrils

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219465 ◽  
Author(s):  
Miki Eto ◽  
Tadafumi Hashimoto ◽  
Takao Shimizu ◽  
Takeshi Iwatsubo
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Amyloid Β ◽  
In Vitro Effects

scholarly journals Characterization of amyloid-β precursor protein intracellular domain-associated transcriptional complexes in SH-SY5Y neurocytes

2012 ◽  
Vol 28 (3) ◽  
pp. 259-270 ◽  
Author(s):  
Wulin Yang ◽  
Amy Yong Chen Lau ◽  
Shuizhong Luo ◽  
Qian Zhu ◽  
Li Lu
Keyword(s):  
Amyloid Β ◽  
Precursor Protein ◽  
Intracellular Domain ◽  
Transcriptional Complexes

Mass Spectrometric Characterization of Amyloid-β Species in the 7PA2 Cell Model of Alzheimer's Disease

2012 ◽  
Vol 33 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Erik Portelius ◽  
Maria Olsson ◽  
Gunnar Brinkmalm ◽  
Ulla Rüetschi ◽  
Niklas Mattsson ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cell Model ◽  
Amyloid Β ◽  
Mass Spectrometric ◽  
Model Of Alzheimer’S Disease ◽  
7Pa2 Cell

scholarly journals Post-mortem analyses of PiB and flutemetamol in diffuse and cored amyloid-β plaques in Alzheimer’s disease

2020 ◽  
Vol 140 (4) ◽  
pp. 463-476
Author(s):  
Milos D. Ikonomovic ◽  
Christopher J. Buckley ◽  
Eric E. Abrahamson ◽  
Julia K. Kofler ◽  
Chester A. Mathis ◽  
...  
Keyword(s):  
Frontal Cortex ◽  
Three Dimensional ◽  
Amyloid Β ◽  
Brain Regions ◽  
Area Coverage ◽  
Amyloid Pet ◽  
Tissue Sections ◽  
Total Fluorescence ◽  
Percent Area ◽  
Diffuse Plaques

Abstract Specificity and sensitivity of positron emission tomography (PET) radiopharmaceuticals targeting fibrillar amyloid-β (Aβ) deposits is high for detection of neuritic Aβ plaques, a mature form of Aβ deposits which often have dense Aβ core (i.e., cored plaques). However, imaging-to-autopsy validation studies of amyloid PET radioligands have identified several false positive cases all of which had mainly diffuse Aβ plaques (i.e., plaques without neuritic pathology or dense amyloid core), and high amyloid PET signal was reported in the striatum where diffuse plaques predominate in Alzheimer’s disease (AD). Relative contributions of different plaque types to amyloid PET signal is unclear, particularly in neocortical areas where they are intermixed in AD. In vitro binding assay and autoradiography were performed using [3H]flutemetamol and [3H]Pittsburgh Compound-B (PiB) in frozen brain homogenates from 30 autopsy cases including sporadic AD and non-AD controls with a range of brain Aβ burden and plaque density. Fixed tissue sections of frontal cortex and caudate from 10 of the AD cases were processed for microscopy using fluorescent derivatives of flutemetamol (cyano-flutemetamol) and PiB (cyano-PiB) and compared to Aβ immunohistochemistry and pan-amyloid (X-34) histology. Using epifluorescence microscopy, percent area coverage and fluorescence output values of cyano-PiB- and cyano-flutemetamol-labeled plaques in two-dimensional microscopic fields were then calculated and combined to obtain integrated density measurements. Using confocal microscopy, we analysed total fluorescence output of the entire three-dimensional volume of individual cored plaques and diffuse plaques labeled with cyano-flutemetamol or cyano-PiB. [3H]Flutemetamol and [3H]PiB binding values in tissue homogenates correlated strongly and their binding pattern in tissue sections, as seen on autoradiograms, overlapped the pattern of Aβ-immunoreactive plaques on directly adjacent sections. Cyano-flutemetamol and cyano-PiB fluorescence was prominent in cored plaques and less so in diffuse plaques. Across brain regions and cases, percent area coverage of cyano-flutemetamol-labeled plaques correlated strongly with cyano-PiB-labeled and Aβ-immunoreactive plaques. For both ligands, plaque burden, calculated as percent area coverage of all Aβ plaque types, was similar in frontal cortex and caudate regions, while integrated density values were significantly greater in frontal cortex, which contained both cored plaques and diffuse plaques, compared to the caudate, which contained only diffuse plaques. Three-dimensional analysis of individual plaques labeled with either ligand showed that total fluorescence output of a single cored plaque was equivalent to total fluorescence output of approximately three diffuse plaques of similar volume. Our results indicate that [18F]flutemetamol and [11C]PiB PET signal is influenced by both diffuse plaques and cored plaques, and therefore is likely a function of plaque size and density of Aβ fibrils in plaques. Brain areas with large volumes/frequencies of diffuse plaques could yield [18F]flutemetamol and [11C]PiB PET retention levels comparable to brain regions with a lower volume/frequency of cored plaques.

scholarly journals Isolation and Characterization of Patient-derived, Toxic, High Mass Amyloid β-Protein (Aβ) Assembly from Alzheimer Disease Brains

2009 ◽  
Vol 284 (47) ◽  
pp. 32895-32905 ◽  
Author(s):  
Akihiko Noguchi ◽  
Satoko Matsumura ◽  
Mari Dezawa ◽  
Mari Tada ◽  
Masako Yanazawa ◽  
...  
Keyword(s):  
Alzheimer Disease ◽  
Amyloid Β ◽  
High Mass ◽  
Amyloid Β Protein ◽  
Isolation And Characterization ◽  
Β Protein
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