Thawed autologous peripheral blood stem cells require modified quantification methods for hematopoietic progenitor cell evaluation

2012 ◽  
Vol 22 (1-3) ◽  
pp. 57-67
Author(s):  
Véronique Decot ◽  
François Alla ◽  
Véronique Latger-Cannard ◽  
Sorin Visanica ◽  
Brigitte Witz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Peripheral Blood ◽  
Progenitor Cell ◽  
Hematopoietic Progenitor Cell ◽  
Hematopoietic Progenitor ◽  
Peripheral Blood Stem Cells ◽  
Blood Stem Cells

scholarly journals Application of Hematopoietic Progenitor Cell Count: To Optimize Peripheral Blood Stem Cell Harvest

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 5837-5837
Author(s):  
Masataka Takeshita ◽  
Risen Hirai ◽  
Akira Tanimura ◽  
Miki Nakamura ◽  
Shotaro Hagiwara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Count ◽  
Peripheral Blood ◽  
Progenitor Cell ◽  
Peripheral Blood Stem Cell ◽  
Blood Stem Cell ◽  
Hematopoietic Progenitor Cell ◽  
Hematopoietic Progenitor ◽  
Cell Harvest

Abstract background. High dose therapy and autogenic or allogeneic stem cell transplantation plays an important role in the treatment course of hematological malignancies. In some countries, major method of unrelated stem cell donation programs had shifted from bone marrow harvest (BM) to peripheral blood stem cell harvest (PBSCH). PBSCH is a heavy duty not only for donor or patient, also for medical staffs. In some cases, poor mobilization may cause poor collection of stem cells. Hemogram needs time for May-Giemsa stain, and CD34 count needs complicated technique and running cost. New simple tool to predict the count of mobilized stem cell is needed to optimize PBSCH. methods. Since 2009, we started measuring peripheral blood hematopoietic progenitor cell (HPC) with Sysmex XE-5000(R) blood cell counter. With IMI channel method, we could rapidly know the count of circulating stem cells. Daily HPC count and yielded CD 34 positive cell count were analyzed. results. 189 samples were collected from 122 donors/patients. Diagnosis of patients: malignant lymphoma (n=29), leukemia (3), multiple myeloma (74), amyloidosis (5), cryoglobulinemia (1). 10 healthy donors were also included. Age: 18-66, Sex: male 82/female 40. Mobilization regimen: G-CSF 57, chemo+G-CSF 74, G-CSF+plerixafor 1 HPC count (cells/ul) and collected CD34 positive cells (106cells/kg) had positive correlation. When HPC count was above 25/ul, collected CD34 positive cells were above 1x106/kg (positive predictive value: 80.9%). Number of PBSCH operation was 1.59 in average. We also show three cases in which HPC count was useful to make clinical decision of initiating PBSCH. discussion. HPC and CD34 had positive correlation, and HPC >25/ul seems to be appropriate cut-off to start PBSCH. With our former threshold of PBSCH (G-CSF day>4, WBC >3000/ul), 241 operations were to be planned. Including HPC count, we could reduce PBSCH operation to 204. Hematopoietic progenitor cell count is rapid and inexpensive method. Within 5 minutes, mobilized stem cells can be measured, and it may be also useful in outpatient-based harvest settings. Figure 1 Figure 1. Disclosures No relevant conflicts of interest to declare.

Effects of glucocorticoids and mineralocorticoids on proliferation and maturation of human peripheral blood stem cells

1999 ◽  
Vol 62 (2) ◽  
pp. 65-73 ◽  
Author(s):  
St�phanie Grafte-Faure ◽  
Catherine Leveque ◽  
Marc Vasse ◽  
Claudine Soria ◽  
Vic Norris ◽  
...  
Keyword(s):  
Stem Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Blood Stem Cells
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