Recellularization potential of small diameter vascular grafts derived from human umbilical artery

2019 ◽  
Vol 30 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Panagiotis Mallis ◽  
Efstathios Michalopoulos ◽  
Paschalis Pantsios ◽  
Foteini Kozaniti ◽  
Despoina Deligianni ◽  
...  
Keyword(s):  
Umbilical Artery ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Human Umbilical Artery ◽  
Small Diameter Vascular Grafts

scholarly journals Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1699
Author(s):  
Chih-Hsun Lin ◽  
Kai Hsia ◽  
Chih-Kuan Su ◽  
Chien-Chin Chen ◽  
Chang-Ching Yeh ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Umbilical Artery ◽  
Vascular Tissue ◽  
Treatment Time ◽  
Umbilical Vein ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Ultrasonic Power ◽  
Human Umbilical Artery

Decellularized vascular grafts are useful for the construction of biological small-diameter tissue-engineered vascular grafts (≤6 mm). Traditional chemical decellularization requires a long treatment time, which may damage the structure and alter the mechanical properties. Decellularization using sonication is expected to solve this problem. The aim of this study was to develop an effective decellularization method using ultrasound followed by washing. Different power values of sonication at 40 kHz were tested for 2, 4, and 8 h followed by a washing procedure. The efficacy of sonication of decellularized human umbilical artery (sDHUA) was evaluated via DNA content, histological staining, mechanical properties, and biocompatibility. The sDHUAs were further implanted into rats for up to 90 days and magnetic resonance angiography (MRA) was performed for the implanted grafts. The results demonstrated that treatment of human umbilical artery (HUA) by sonication at ultrasonic power of 204 W for 4 h followed by washing for 24 h in 2% SDS buffer could eliminate more than 90% of cells and retain similar mechanical properties of the HUA. Recellularization was assessed by scanning electron microscopy (SEM), which indicated that sDHUA provided niches for human umbilical vein endothelial cells (HUVECs) to reside, indicating in vitro cytocompatibility. Further implantation tests also indicated the fitness of the sonication-treated HUA as a scaffold for small-caliber tissue engineering vascular grafts.

scholarly journals Comparative Evaluation on Impacts of Fibronectin, Heparin–Chitosan, and Albumin Coating of Bacterial Nanocellulose Small-Diameter Vascular Grafts on Endothelialization In Vitro

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1952
Author(s):  
Max Wacker ◽  
Jan Riedel ◽  
Heike Walles ◽  
Maximilian Scherner ◽  
George Awad ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Density Lipoprotein ◽  
Human Albumin ◽  
Water Soluble ◽  
Bacterial Nanocellulose ◽  
Functionally Impaired ◽  
Small Diameter Vascular Grafts

In this study, we contrast the impacts of surface coating bacterial nanocellulose small-diameter vascular grafts (BNC-SDVGs) with human albumin, fibronectin, or heparin–chitosan upon endothelialization with human saphenous vein endothelial cells (VEC) or endothelial progenitor cells (EPC) in vitro. In one scenario, coated grafts were cut into 2D circular patches for static colonization of a defined inner surface area; in another scenario, they were mounted on a customized bioreactor and subsequently perfused for cell seeding. We evaluated the colonization by emerging metabolic activity and the preservation of endothelial functionality by water soluble tetrazolium salts (WST-1), acetylated low-density lipoprotein (AcLDL) uptake assays, and immune fluorescence staining. Uncoated BNC scaffolds served as controls. The fibronectin coating significantly promoted adhesion and growth of VECs and EPCs, while albumin only promoted adhesion of VECs, but here, the cells were functionally impaired as indicated by missing AcLDL uptake. The heparin–chitosan coating led to significantly improved adhesion of EPCs, but not VECs. In summary, both fibronectin and heparin–chitosan coatings could beneficially impact the endothelialization of BNC-SDVGs and might therefore represent promising approaches to help improve the longevity and reduce the thrombogenicity of BNC-SDVGs in the future.

scholarly journals Extracellular Matrix for Small-Diameter Vascular Grafts

2020 ◽  
Vol 26 (23-24) ◽  
pp. 1388-1401
Author(s):  
Megan Kimicata ◽  
Prateek Swamykumar ◽  
John P. Fisher
Keyword(s):  
Extracellular Matrix ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Small Diameter Vascular Grafts

Tissue engineered small-diameter vascular grafts

2003 ◽  
Vol 30 (4) ◽  
pp. 507-517 ◽  
Author(s):  
Rachael H Schmedlen ◽  
Wafa M Elbjeirami ◽  
Andrea S Gobin ◽  
Jennifer L West
Keyword(s):  
Vascular Grafts ◽  
Small Diameter ◽  
Small Diameter Vascular Grafts

Preliminary steps for the creation of small diameter vascular grafts

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S41-S42
Author(s):  
S. Perez Lopez ◽  
M. Navarro Rego ◽  
M. Álvarez Viejo ◽  
M. Perez Basterrechea ◽  
J. Cenis Anadon ◽  
...  
Keyword(s):  
Vascular Grafts ◽  
Small Diameter ◽  
The Creation ◽  
Small Diameter Vascular Grafts

Development of Small-Diameter Vascular Grafts

2007 ◽  
Vol 31 (4) ◽  
pp. 682-689 ◽  
Author(s):  
Xinwen Wang ◽  
Peter Lin ◽  
Qizhi Yao ◽  
Changyi Chen
Keyword(s):  
Vascular Grafts ◽  
Small Diameter ◽  
Small Diameter Vascular Grafts

Intensive Statin Therapy: A Favorable Adjunct to the Improvement of Small-Diameter Vascular Grafts

Angiology ◽  
2010 ◽  
Vol 61 (5) ◽  
pp. 427-436 ◽  
Author(s):  
Jie Li ◽  
Wei-Ming Lu ◽  
Xiao-Xi Li ◽  
Shen-Ming Wang ◽  
Jian-Xing Yu ◽  
...  
Keyword(s):  
Statin Therapy ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Intensive Statin Therapy ◽  
Small Diameter Vascular Grafts
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