scholarly journals Increased sensitivity of 3D-Well enzyme-linked immunosorbent assay (ELISA) for infectious disease detection using 3D-printing fabrication technology

2015 ◽  
Vol 26 (s1) ◽  
pp. S45-S53 ◽  
Author(s):  
Harpal Singh ◽  
Masayuki Shimojima ◽  
Shuetsu Fukushi ◽  
An Le Van ◽  
Masami Sugamata ◽  
...  
Keyword(s):  
Infectious Disease ◽  
3D Printing ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Disease Detection ◽  
Fabrication Technology ◽  
Increased Sensitivity

scholarly journals Peptide-Based OspC Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Lyme Borreliosis

1998 ◽  
Vol 36 (12) ◽  
pp. 3474-3479 ◽  
Author(s):  
Marianne J. Mathiesen ◽  
Michael Christiansen ◽  
Klaus Hansen ◽  
Arne Holm ◽  
Eva Åsbrink ◽  
...  
Keyword(s):  
Lyme Borreliosis ◽  
Immunosorbent Assay ◽  
Erythema Migrans ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Amino Acid Residues ◽  
Serum Samples ◽  
Igg Antibody ◽  
Acrodermatitis Chronica Atrophicans ◽  
Increased Sensitivity

Sera from 210 patients with Lyme borreliosis (LB) were studied by an enzyme-linked immunosorbent assay (ELISA) based on a synthetic peptide (pepC10) comprising the C-terminal 10-amino-acid residues of OspC of Borrelia burgdorferi. We found that 36.3 and 45.0% of the serum samples from patients with erythema migrans (EM) and neuroborreliosis (NB), respectively, displayed immunoglobulin M (IgM) anti-pepC10 reactivities, while these samples rarely (≤8%) displayed IgG antibody reactivities. Sera from patients with acrodermatitis chronica atrophicans did not contain anti-pepC10 antibodies. The diagnostic performance of this newly developed peptide ELISA was compared with those of an ELISA based on the full-length recombinant OspC protein (rOspC) and a commercially available ELISA based on theB. burgdorferi flagellum (Fla). The sensitivity of the IgM pepC10 ELISA was slightly lower (P < 0.04) than that of the rOspC ELISA for EM patients (36.3 versus 43.8%), while there was no difference for NB patients (45.0 versus 48.0%). However, the optical density values obtained by the pepC10 ELISA were generally higher than those obtained by the rOspC ELISA, leading to a significantly better quantitative discrimination between seropositive patients with NB and controls (P < 0.008). The specificity of the pepC10 ELISA was similar to those of the rOspC ELISA and the Fla ELISA for relevant controls including patients with syphilis and mononucleosis. Although the overall diagnostic sensitivity of the Fla ELISA was superior, 8.8 and 12.0% of the EM and NB patients, respectively, were antibody positive only by the pepC10 ELISA. Thus, use of a diagnostic test for LB based on the detection of IgM antibodies to pepC10 and Fla has increased sensitivity for the diagnosis of early LB.

scholarly journals Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects

2020 ◽  
Author(s):  
Chalisa Jaturapaktrarak ◽  
Penpan Payattikul ◽  
Tassanee Lohnoo ◽  
Yothin Kumsang ◽  
Aree Laikul ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Early Diagnosis ◽  
Immunosorbent Assay ◽  
Protein A ◽  
Assay Development ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Pythium Insidiosum ◽  
Prompt Treatment ◽  
Optimized Protocol

Abstract Objectives: Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal pythiosis are on the rise worldwide. Prognosis of the pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in both humans and animals. This study aims at reporting an optimized protocol for the development of a protein A/G-based enzyme-linked immunosorbent assay (ELISA) for the detection of anti- P. insidiosum antibody in multiple host species. Results: A total of 25 pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti- P. insidiosum antibodies in serum samples of both humans and animals. It is a versatile, feasible-to-develop, and functional immunodiagnostic assay for pythiosis.

scholarly journals Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects

2020 ◽  
Author(s):  
Chalisa Jaturapaktrarak ◽  
Penpan Payattikul ◽  
Tassanee Lohnoo ◽  
Yothin Kumsang ◽  
Aree Laikul ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Early Diagnosis ◽  
Immunosorbent Assay ◽  
Protein A ◽  
Assay Development ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Pythium Insidiosum ◽  
Prompt Treatment ◽  
Optimized Protocol

Abstract Objectives: Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal pythiosis are on the rise worldwide. Prognosis of the pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in both humans and animals. This study aims at reporting an optimized protocol for the development of a protein A/G-based enzyme-linked immunosorbent assay (ELISA) for the detection of anti-P. insidiosum antibody in multiple host species. Results: A total of 25 pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti-P. insidiosum antibodies in serum samples of both humans and animals. It is a versatile and feasible-to-develop immunodiagnostic assay for pythiosis.

scholarly journals Application of 3D Printing Technology in Increasing the Diagnostic Performance of Enzyme-Linked Immunosorbent Assay (ELISA) for Infectious Diseases

Sensors ◽  
2015 ◽  
Vol 15 (7) ◽  
pp. 16503-16515 ◽  
Author(s):  
Harpal Singh ◽  
Masayuki Shimojima ◽  
Tomomi Shiratori ◽  
Le Van An ◽  
Masami Sugamata ◽  
...  
Keyword(s):  
3D Printing ◽  
Infectious Diseases ◽  
Immunosorbent Assay ◽  
Diagnostic Performance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Printing Technology ◽  
3D Printing Technology
Sign in / Sign up

Export Citation Format

Share Document