scholarly journals Multiplex Real-Time Reverse Transcription PCR for Influenza A Virus, Influenza B Virus, and Severe Acute Respiratory Syndrome Coronavirus 2

2021 ◽  
Vol 27 (7) ◽  
pp. 1821-1830
Author(s):  
Bo Shu ◽  
Marie K. Kirby ◽  
William G. Davis ◽  
Christine Warnes ◽  
Jimma Liddell ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Real Time ◽  
Influenza A Virus ◽  
Reverse Transcription ◽  
Influenza A ◽  
Influenza B Virus ◽  
Influenza B ◽  
Reverse Transcription Pcr ◽  
B Virus ◽  
Virus Influenza

scholarly journals Clinical Investigations. Comparison of Directigen Flu A+B with Real Time PCR in the Diagnosis of Influenza / Сравнение Иммунохроматографического Метода (Directigen Flu A+B) И Теста RT-PCR При Инфекциях Гриппа

Folia Medica ◽  
2015 ◽  
Vol 57 (2) ◽  
pp. 104-110 ◽  
Author(s):  
Golubinka Bosevska ◽  
Nikola Panovski ◽  
Elizabeta Janceska ◽  
Vladimir Mikik ◽  
Irena Kondova Topuzovska ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A Virus ◽  
Real Time Pcr ◽  
Influenza A ◽  
Age Groups ◽  
Influenza Viruses ◽  
Influenza B Virus ◽  
Influenza B ◽  
B Virus ◽  
Nose And Throat

AbstractEarly diagnosis and treatment of patients with influenza is the reason why physicians need rapid high-sensitivity influenza diagnostic tests that require no complex lab equipment and can be performed and interpreted within 15 min. The Aim of this study was to compare the rapid Directigen Flu A+B test with real time PCR for detection of influenza viruses in the Republic of Macedonia. MATERIALS AND METHODS: One-hundred-eight respiratory samples (combined nose and throat swabs) were routinely collected for detection of influenza virus during influenza seasons. Forty-one patients were pediatric cases and 59 were adult. Their mean age was 23 years. The patients were allocated into 6 age groups: 0 - 4 yrs, 5 - 9 yrs, 10 - 14 yrs, 15 - 19 yrs, 20-64 yrs and > 65 yrs. Each sample was tested with Directigen Flu A+B and CDC real time PCR kit for detection and typisation/subtypisation of influenza according to the lab diagnostic protocol. RESULTS: Directigen Flu A+B identified influenza A virus in 20 (18.5%) samples and influenza B virus in two 2 (1.9%) samples. The high specificity (100%) and PPV of Directigen Flu A+B we found in our study shows that the positive results do not need to be confirmed. The overall sensitivity of Directigen Flu A+B is 35.1% for influenza A virus and 33.0% for influenza B virus. The sensitivity for influenza A is higher among children hospitalized (45.0%) and outpatients (40.0%) versus adults. CONCLUSION: Directigen Flu A+B has relatively low sensitivity for detection of influenza viruses in combined nose and throat swabs. Negative results must be confirmed.

scholarly journals O VÍRUS INFLUENZA: REVISÃO NARRATIVA DA LITERATURA

2020 ◽  
Vol 3 (2) ◽  
pp. 74
Author(s):  
Gatzke Fernanda ◽  
Vera Regina Medeiros Andrade
Keyword(s):  
Influenza Virus ◽  
Influenza Vaccine ◽  
Influenza A Virus ◽  
Influenza A ◽  
Influenza B Virus ◽  
Influenza B ◽  
Electronic Library ◽  
B Virus ◽  
Virus Influenza

<p><strong>Introdução</strong>: o vírus Influenza é um patógeno respiratório humano que causa infecções sazonais e endêmicas e pandemias periódicas. No século XX, a pior pandemia registrada foi em 1918 que matou aproximadamente milhões de pessoas em todo o mundo. É um vírus envelopado de RNA de cadeia simples de sentido negativo segmentado. Os principais sintomas causados por este vírus são caracterizados por um início súbito de febre alta, dor de cabeça, coriza, tosse e inflamação das vias respiratórias que persistem por 7 a 10 dias. <strong>Objetivo</strong>: realizar uma revisão narrativa da literatura sobre o vírus Influenza. <strong>Metodologia</strong>: revisão exploratória e narrativa da literatura, sem definição de critérios explícitos de seleção dos artigos, nas bases de dados eletrônicas PubMed, Scientific Electronic Library Online (Scielo), Literatura Latino-americana e do Caribe em Ciências da Saúde (LILACS), utilizando palavras chave como Influenza, Influenza vírus, Influenza A vírus, Influenza B vírus, Influenza vaccine, vacina contra Influenza; historical Influenza review, história Influenza. <strong>Considerações</strong>: os vírus Influenza são uma ameaça à saúde, porque a forma rápida de disseminação dificulta as estratégias de vacinação. Atualmente, dois subtipos do vírus da Influenza A, o H1N1 e o H3N2 estão em circulação. As epidemias ocorrem por causa da variação antigênica viral, proteção para essas cepas circulantes e o grau de virulência desses novos vírus. É importante a conscientização das pessoas com a prevenção para evitar problemas de saúde pública.</p>

scholarly journals Detection of severe acute respiratory syndrome coronavirus 2 and influenza viruses based on CRISPR-Cas12a

2020 ◽  
pp. 153537022096379
Author(s):  
Oraphan Mayuramart ◽  
Pattaraporn Nimsamer ◽  
Somruthai Rattanaburi ◽  
Naphat Chantaravisoot ◽  
Kritsada Khongnomnan ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Severe Acute Respiratory Syndrome ◽  
Influenza A ◽  
Limit Of Detection ◽  
Cross Reactivity ◽  
Influenza Viruses ◽  
Influenza B Virus ◽  
Influenza B ◽  
Influenza Virus A ◽  
B Virus

Due to the common symptoms of COVID-19, patients are similar to influenza-like illness. Therefore, the detection method would be crucial to discriminate between SARS-CoV-2 and influenza virus-infected patients. In this study, CRISPR-Cas12a-based detection was applied for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus, and influenza B virus which would be a practical and attractive application for screening of patients with COVID-19 and influenza in areas with limited resources. The limit of detection for SARS-CoV-2, influenza A, and influenza B detection was 10, 103, and 103 copies/reaction, respectively. Moreover, the assays yielded no cross-reactivity against other respiratory viruses. The results revealed that the detection of influenza virus and SARS-CoV-2 by using RT-RPA and CRISPR-Cas12a technology reaches 96.23% sensitivity and 100% specificity for SARS-CoV-2 detection. The sensitivity for influenza virus A and B detections was 85.07% and 94.87%, respectively. In addition, the specificity for influenza virus A and B detections was approximately 96%. In conclusion, the RT-RPA with CRISPR-Cas12a assay was an effective method for the screening of influenza viruses and SARS-CoV-2 which could be applied to detect other infectious diseases in the future.

scholarly journals STUDIES ON THE MECHANISM OF ADAPTATION OF INFLUENZA VIRUS TO MICE

1947 ◽  
Vol 86 (5) ◽  
pp. 357-366 ◽  
Author(s):  
George K. Hirst
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
High Titer ◽  
Strain Difference ◽  
Mouse Lung ◽  
Influenza B Virus ◽  
Influenza B ◽  
Difference Problem ◽  
B Virus ◽  
Repeated Passage

1. When strains of influenza A virus which have been isolated in chick embryos are introduced into the mouse lung, the virus multiplies readily and achieves initially a titer which is as high as is even obtained, even after repeated passage. The high initial titer of virus may be unaccompanied by any lethal or visible pathogenic effects; but with four or five mouse passages the agent becomes lethal in high titer and causes extensive pulmonary consolidation, though its capacity to multiply in the lung has not increased. In one example the adaptation to mouse lung was accompanied by increasing capacity to agglutinate guinea pig red cells without a corresponding increase in agglutinating power for chicken cells. Influenza B virus, in preliminary tests, did not behave in a similar fashion. 2. The adaptation of influenza A virus to mice is accompanied by changes in antigenic pattern, as detected by cross-tests with the agglutination inhibition method. Two strains, initially similar, with passage, changed in pattern along divergent paths so that they became not only unlike the parent strains but unlike each other. This finding has important implications for the interpretation of the strain difference problem in human influenza.

scholarly journals Incoming Influenza A Virus Evades Early Host Recognition, while Influenza B Virus Induces Interferon Expression Directly upon Entry

2012 ◽  
Vol 86 (20) ◽  
pp. 11183-11193 ◽  
Author(s):  
P. Osterlund ◽  
M. Strengell ◽  
L. P. Sarin ◽  
M. M. Poranen ◽  
R. Fagerlund ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Host Recognition ◽  
Influenza B Virus ◽  
Influenza B ◽  
B Virus
Sign in / Sign up

Export Citation Format

Share Document