scholarly journals Dolphin Morbillivirus in Eurasian Otters, Italy

2019 ◽  
Vol 25 (2) ◽  
pp. 372-374 ◽  
Author(s):  
Iolanda Padalino ◽  
Giovanni Di Guardo ◽  
Antonio Carbone ◽  
Pasquale Troiano ◽  
Antonio Parisi ◽  
...  
Keyword(s):  
Dolphin Morbillivirus

Dolphin Morbillivirus in the Mediterranean Sea

2008 ◽  
Vol 34 (4) ◽  
pp. 514-515 ◽  
Author(s):  
Giovanni Di Guardo
Keyword(s):  
Mediterranean Sea ◽  
Dolphin Morbillivirus ◽  
The Mediterranean

Localization of Viral Antigens and Brain Ultrastructural Findings in Striped Dolphins (Stenella coeruleoalba) with ‘Brain-Only’ Dolphin morbillivirus Infection

2017 ◽  
Vol 156 (1) ◽  
pp. 111
Author(s):  
G. Di Guardo ◽  
R. Giacominelli-Stuffler ◽  
M. Baffoni ◽  
G. Pietroluongo ◽  
C.E. Di Francesco ◽  
...  
Keyword(s):  
Stenella Coeruleoalba ◽  
Viral Antigens ◽  
Dolphin Morbillivirus ◽  
Ultrastructural Findings

scholarly journals Dolphin Morbillivirus and Toxoplasma gondii coinfection in a Mediterranean fin whale (Balaenoptera physalus)

2012 ◽  
Vol 8 (1) ◽  
pp. 20 ◽  
Author(s):  
Sandro Mazzariol ◽  
Federica Marcer ◽  
Walter Mignone ◽  
Laura Serracca ◽  
Mariella Goria ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Balaenoptera Physalus ◽  
Fin Whale ◽  
Dolphin Morbillivirus

Expression from baculovirus and serological reactivity of the nucleocapsid protein of dolphin morbillivirus

2010 ◽  
Vol 143 (2-4) ◽  
pp. 384-388 ◽  
Author(s):  
Rebecca J. Grant ◽  
Karen L. Kelley ◽  
James E. Maruniak ◽  
Alejandra Garcia-Maruniak ◽  
Tom Barrett ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Dolphin Morbillivirus ◽  
Serological Reactivity

scholarly journals Specific capture and whole-genome phylogeography of Dolphin morbillivirus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Francesco Cerutti ◽  
Federica Giorda ◽  
Carla Grattarola ◽  
Walter Mignone ◽  
Chiara Beltramo ◽  
...  
Keyword(s):  
Illumina Miseq ◽  
Whole Genome ◽  
The Novel ◽  
Target Enrichment ◽  
Genome Sequences ◽  
Tissue Samples ◽  
Dolphin Morbillivirus ◽  
Field Samples ◽  
The Mediterranean ◽  
Phylodynamic Analysis

AbstractDolphin morbillivirus (DMV) is considered an emerging threat having caused several epidemics worldwide. Only few DMV genomes are publicly available. Here, we report the use of target enrichment directly from cetacean tissues to obtain novel DMV genome sequences, with sequence comparison and phylodynamic analysis. RNA from 15 tissue samples of cetaceans stranded along the Italian and French coasts (2008–2017) was purified and processed using custom probes (by bait hybridization) for target enrichment and sequenced on Illumina MiSeq. Data were mapped against the reference genome, and the novel sequences were aligned to the available genome sequences. The alignment was then used for phylogenetic and phylogeographic analysis using MrBayes and BEAST. We herein report that target enrichment by specific capture may be a successful strategy for whole-genome sequencing of DMV directly from field samples. By this strategy, 14 complete and one partially complete genomes were obtained, with reads mapping to the virus up to 98% and coverage up to 7800X. The phylogenetic tree well discriminated the Mediterranean and the NE-Atlantic strains, circulating in the Mediterranean Sea and causing two different epidemics (2008–2015 and 2014–2017, respectively), with a limited time overlap of the two strains, sharing a common ancestor approximately in 1998.

scholarly journals Dolphin Morbillivirus Epizootic Resurgence, Mediterranean Sea

2008 ◽  
Vol 14 (3) ◽  
pp. 471-473 ◽  
Author(s):  
Juan-Antonio Raga ◽  
Ashley Banyard ◽  
Mariano Domingo ◽  
Mandy Corteyn ◽  
Marie-Françoise Van Bressem ◽  
...  
Keyword(s):  
Mediterranean Sea ◽  
Dolphin Morbillivirus

scholarly journals Mediterranean Fin Whales (Balaenoptera physalus) Threatened by Dolphin MorbilliVirus

2015 ◽  
Vol 22 (2) ◽  
pp. 302-305 ◽  
Author(s):  
Sandro Mazzariol ◽  
Cinzia Centelleghe ◽  
Giorgia Beffagna ◽  
Michele Povinelli ◽  
Giuliana Terracciano ◽  
...  
Keyword(s):  
Balaenoptera Physalus ◽  
Dolphin Morbillivirus ◽  
Fin Whales

Epizootic of dolphin morbillivirus on the Catalonian Mediterranean coast in 2007

2011 ◽  
Vol 169 (4) ◽  
pp. 102-102 ◽  
Author(s):  
S. Soto ◽  
R. Gonzalez ◽  
F. Alegre ◽  
B. Gonzalez ◽  
P. Medina ◽  
...  
Keyword(s):  
Mediterranean Coast ◽  
Dolphin Morbillivirus

Retrospective immunohistochemical investigation on dolphin morbillivirus infection by comparing the performance of heterologous monoclonal and polyclonal antibodies – Short communication

2021 ◽  
Author(s):  
Federica Giorda ◽  
Giovanni Di Guardo ◽  
Katia Varello ◽  
Alessandra Pautasso ◽  
Eva Sierra ◽  
...  
Keyword(s):  
Short Communication ◽  
Polyclonal Antibodies ◽  
Cross Reactivity ◽  
Binding Activity ◽  
N Protein ◽  
Target Organs ◽  
Dolphin Morbillivirus ◽  
Free Ranging ◽  
The Brain ◽  
Histology And Immunohistochemistry

AbstractDolphin morbillivirus (DMV) is a pathogen of great concern in free-ranging cetaceans. Confirmation and staging of morbillivirus infections rely on histology and immunohistochemistry (IHC), following molecular detection. As at the present time no specific antibodies (Abs) against DMV are available, two heterologous Abs have been used worldwide for the examinations of morbillivirus infections of cetaceans. One is a monoclonal Ab (MoAb) prepared against the N protein of canine distemper virus (CDV), whereas the other is a polyclonal Ab raised in rabbits against rinderpest virus (RPV). Both Abs are known to show cross-reactivity with DMV. In this study we compared the labelling quality and the neuroanatomical distribution of staining with these two Abs by means of IHC analysis. To this end, serial sections of the target organs from ten free-ranging stranded cetaceans, previously diagnosed as being infected with DMV by PCR and/or serology, were subjected to IHC. The brain, lungs and lymph nodes of one animal were found to be positive with both Abs. From two other animals, the brain and the spleen, respectively, tested positive only with the polyclonal Ab. In the positive brain tissues, multifocal immunostaining was observed, with similar staining location and extent, with the two antibodies tested. Our results suggest that the polyclonal anti-RPV Ab might have a stronger binding activity to DMV than the anti-CDV MoAb. Nevertheless, the elaboration and use of specific anti-DMV Abs might be essential to guarantee conclusive results in diagnostic and pathogenetic investigations.

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