scholarly journals Ferrets as Models for Influenza Virus Transmission Studies and Pandemic Risk Assessments

2018 ◽  
Vol 24 (6) ◽  
pp. 965-971 ◽  
Author(s):  
Jessica A. Belser ◽  
Wendy Barclay ◽  
Ian Barr ◽  
Ron A.M. Fouchier ◽  
Ryota Matsuyama ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Transmission ◽  
Risk Assessments ◽  
Transmission Studies

scholarly journals The Pause on Avian H5N1 Influenza Virus Transmission Research Should Be Ended

mBio ◽  
2012 ◽  
Vol 3 (5) ◽  
Author(s):  
Ron A. M. Fouchier ◽  
Adolfo García-Sastre ◽  
Yoshihiro Kawaoka
Keyword(s):  
Influenza Virus ◽  
Risk Mitigation ◽  
Virus Transmission ◽  
Mitigation Measures ◽  
H5n1 Influenza Virus ◽  
Pandemic Preparedness ◽  
Important Work ◽  
H5n1 Influenza ◽  
Transmission Studies ◽  
Avian H5n1 Influenza Virus

ABSTRACT A voluntary 60-day pause on avian H5N1 influenza virus transmission research was announced in January 2012 by the international community of influenza scientists engaged in this work to provide time to explain the benefits of such work and the risk mitigation measures in place. Subsequently, the pause was extended to allow for time for review of the biosafety and biosecurity conditions. After almost 8 months, these conditions have been met in some countries and are close to being met in others. Because H5N1 virus transmission studies are essential for pandemic preparedness, researchers who have approval from their governments and institutions to conduct this research safely under appropriate biosecurity conditions should resume this important work.

Pathogenicity and transmission studies of H7N7 avian influenza virus isolated from feces of magpie origin in chickens and magpie

2010 ◽  
Vol 141 (3-4) ◽  
pp. 268-274 ◽  
Author(s):  
Min-Chul Kim ◽  
Ok-Mi Jeong ◽  
Hyun-Mi Kang ◽  
Mi-Ra Paek ◽  
Ji-Sun Kwon ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Transmission Studies

scholarly journals H3N2 Influenza Virus Transmission from Swine to Turkeys, United States

2004 ◽  
Vol 10 (12) ◽  
pp. 2156-2160 ◽  
Author(s):  
Young K. Choi ◽  
Jee H. Lee ◽  
Gene Erickson ◽  
Sagar M. Goyal ◽  
Han S. Joo ◽  
...  
Keyword(s):  
United States ◽  
Influenza Virus ◽  
Virus Transmission ◽  
H3n2 Influenza ◽  
H3n2 Influenza Virus

scholarly journals Swine-Origin H1 Influenza Viruses Isolated from Humans Exhibit Sustained Infectivity in an Aerosol State

2019 ◽  
Vol 85 (10) ◽  
Author(s):  
Joanna A. Pulit-Penaloza ◽  
Jessica A. Belser ◽  
Terrence M. Tumpey ◽  
Taronna R. Maines
Keyword(s):  
Influenza Virus ◽  
Swine Influenza Virus ◽  
Virus Transmission ◽  
Swine Influenza ◽  
Influenza Viruses ◽  
Aerosol State ◽  
The Stability ◽  
Higher Doses ◽  
Enhanced Stability

ABSTRACT The relative importance of influenza virus transmission via aerosols is not fully understood, but experimental data suggest that aerosol transmission may represent a critical mode of influenza virus spread among humans. Decades ago, prototypical laboratory strains of influenza were shown to persist in aerosols; however, there is a paucity of data available covering currently circulating influenza viruses, which differ significantly from their predecessors. In this study, we evaluated the longevity of influenza viruses in aerosols generated in the laboratory. We selected a panel of H1 viruses that exhibit diverse transmission profiles in the ferret model, including four human isolates of swine origin (referred to as variant) and a seasonal strain. By measuring the ratio of viral RNA to infectious virus maintained in aerosols over time, we show that influenza viruses known to transmit efficiently through the air display enhanced stability in an aerosol state for prolonged periods compared to those viruses that do not transmit as efficiently. We then assessed whether H1 influenza virus was still capable of infecting and causing disease in ferrets after being aged in suspended aerosols. Ferrets exposed to very low levels of influenza virus (≤17 PFU) in aerosols aged for 15 or 30 min became infected, with five of six ferrets shedding virus in nasal washes at titers on par with ferrets who inhaled higher doses of unaged influenza virus. We describe here an underreported characteristic of influenza viruses, stability in aerosols, and make a direct connection to the role this characteristic plays in influenza transmission. IMPORTANCE Each time a swine influenza virus transmits to a human, it provides an opportunity for the virus to acquire adaptations needed for sustained human-to-human transmission. Here, we use aerobiology techniques to test the stability of swine-origin H1 subtype viruses in aerosols and evaluate their infectivity in ferrets. Our results show that highly transmissible influenza viruses display enhanced stability in an aerosol state compared to viruses that do not transmit as efficiently. Similar to human-adapted strains, swine-origin influenza viruses are infectious in ferrets at low doses even after prolonged suspension in the air. These data underscore the risk of airborne swine-origin influenza viruses and support the need for continued surveillance and refinement of innovative laboratory methods to investigate mammalian exposure to inhaled pathogens. Determination of the molecular markers that affect the longevity of airborne influenza viruses will improve our ability to quickly identify emerging strains that present the greatest threat to public health.

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