scholarly journals Frequent Hepatitis E Virus Contamination in Food Containing Raw Pork Liver, France

2014 ◽  
Vol 20 (11) ◽  
pp. 1925-1927 ◽  
Author(s):  
Nicole Pavio ◽  
Thiziri Merbah ◽  
Anne Thébault
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Contamination ◽  
Pork Liver

scholarly journals Foodborne Transmission of Hepatitis E Virus from Raw Pork Liver Sausage, France

2014 ◽  
Vol 20 (11) ◽  
pp. 1945-1947 ◽  
Author(s):  
Christophe Renou ◽  
Anne-Marie Roque-Afonso ◽  
Nicole Pavio
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Pork Liver

scholarly journals Interlaboratory Validation of a Detection Method for Hepatitis E Virus RNA in Pig Liver

2020 ◽  
Vol 8 (10) ◽  
pp. 1460
Author(s):  
Eva Trojnar ◽  
Matthias Contzen ◽  
Dominik Moor ◽  
Anja Carl ◽  
Sabine Burkhardt ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Detection Method ◽  
Collaborative Study ◽  
False Negative ◽  
Rna Extraction ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Liver Sample ◽  
Pig Liver ◽  
Pork Liver

Background: In the last years, the number of notified hepatitis E cases in humans has continuously increased in Europe. Foodborne infection with the zoonotic hepatitis E virus (HEV) genotype 3 is considered the major cause of this disease. Undercooked liver and raw sausages containing the liver of pigs and wild boar are at high risk of containing HEV. However, so far, no standardized method for the detection of HEV-RNA in pig liver is available. Methods: An international collaborative study on method reproducibility involving 11 laboratories was performed for an HEV-RNA detection method, which consists of steps of sample homogenization, RNA extraction and real-time RT-PCR detection, including a process control. Naturally contaminated pork liver samples containing two different amounts of HEV and a HEV-negative pork liver sample were tested by all laboratories using the method. Results: Valid results were retrieved from 10 laboratories. A specificity of 100% and a sensitivity of 79% were calculated for the method. False negative results were only retrieved from the sample containing very low HEV amounts near the detection limit. Conclusions: The results show that the method is highly specific, sufficiently sensitive and robust for use in different laboratories. The method can, therefore, be applied to routine food control as well as in monitoring studies.

scholarly journals Surveillance of Hepatitis E Virus Contamination in Shellfish in China

2015 ◽  
Vol 12 (2) ◽  
pp. 2026-2036 ◽  
Author(s):  
Shenyang Gao ◽  
Dandan Li ◽  
Enhui Zha ◽  
Tiezhong Zhou ◽  
Shen Wang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Contamination

scholarly journals Hepatitis E Virus in Pork Liver Sausage, France

2012 ◽  
Vol 19 (2) ◽  
pp. 264-266 ◽  
Author(s):  
Alessandra Berto ◽  
Sylvia Grierson ◽  
Renate Hakze-van der Honing ◽  
Francesca Martelli ◽  
Reimar Johne ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Pork Liver

scholarly journals Detection of hepatitis E virus in pork liver sausages

2015 ◽  
Vol 193 ◽  
pp. 29-33 ◽  
Author(s):  
Ilaria Di Bartolo ◽  
Giorgia Angeloni ◽  
Eleonora Ponterio ◽  
Fabio Ostanello ◽  
Franco Maria Ruggeri
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Pork Liver

scholarly journals Seroprevalence in blood donors reveals widespread, multi-source exposure to hepatitis E virus, southern France, October 2011

2015 ◽  
Vol 20 (19) ◽  
Author(s):  
J M Mansuy ◽  
K Sauné ◽  
H Rech ◽  
F Abravanel ◽  
C Mengelle ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Blood Donors ◽  
Geographical Area ◽  
Hepatitis E ◽  
Developed Countries ◽  
Southern France ◽  
Igm Antibodies ◽  
Specific Questionnaire ◽  
Pork Liver ◽  
Epidemiological Information

The apparent seroprevalence of hepatitis E Virus (HEV) varies greatly among developed countries depending on the geographical area and the sensitivity of immunoassays. We used a validated assay to determine the prevalence of HEV IgG and IgM antibodies among 3,353 blood donors living in southern France, who gave blood during the two first weeks of October 2011 and participated in the study. Demographic and epidemiological information was collected using a specific questionnaire. We also screened 591 samples for HEV RNA. Overall IgG seroprevalence was 39.1% and varied from 20% to 71.3% depending on the geographical area (p?<?0.001) while IgM seroprevalence was 3.31%. Anti-HEV IgG was significantly correlated with increasing age (p?<?0.001), eating uncooked pork liver sausages (p?<?0.001), offal (p?=?0.003), or mussels (p?=?0.02). Anti-HEV IgM was associated with being male (p?=?0.01) and eating uncooked pork liver sausages (p?=?0.02). HEV RNA was detected in one of the 99 anti-HEV IgM-positive samples, but in none of the 492 anti-HEV IgM-negative samples. HEV is hyperendemic in southern France. Dietary and culinary habits alone cannot explain the epidemiology of HEV in this region, indicating that other modes of contamination should be investigated.

Hepatitis E Virus in LeberBiopsien von Patienten mit akuter Hepatitis unbekannter Ätiologie

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
C Dorloff ◽  
J Hemberger ◽  
M Odenthal ◽  
H Holzmann ◽  
S Aberle ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E

Severe course of Hepatitis E Virus infection in a patient with pre-existing autoimmune liver disease

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
S Schlosser ◽  
J Pflaum ◽  
K Weigand ◽  
JJ Wenzel ◽  
W Jilg ◽  
...  
Keyword(s):  
Liver Disease ◽  
Virus Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Autoimmune Liver Disease
Sign in / Sign up

Export Citation Format

Share Document