scholarly journals Identifying, discriminating and isolating cultivars of ‘Marguerites’ originated from Argyranthemum frutescens parentages and their intergeneric and interspecific hybridities by DNA markers amplified by RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat)∗

2014 ◽  
Vol 9 (4) ◽  
pp. 97-112 ◽  
Author(s):  
Masahiro Morikawa ◽  
Takahiro Ｍuto ◽  
Arnoldo Santos-Guerrra ◽  
Katsuhiko Kondo
Keyword(s):  
Dna Markers ◽  
Simple Sequence Repeat ◽  
Random Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence

scholarly journals Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs

2011 ◽  
Vol 42 (No. 3) ◽  
pp. 87-94 ◽  
Author(s):  
U.K. Posselt ◽  
P. Barre ◽  
G. Brazauskas ◽  
L.B. Turner
Keyword(s):  
Perennial Ryegrass ◽  
Simple Sequence Repeat ◽  
Fragment Length Polymorphism ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Correlation Coefficients ◽  
Inter Simple Sequence Repeat ◽  
Grass Species ◽  
Sequence Repeat ◽  
Simple Sequence

Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for AFLPs, ISSRs, RAPDs and SSRs, respectively. Cophenetic values resulted in good (AFLP and SSR-B = 0.88) to moderately good fits (ISSR = 0.76, RAPD = 0.70, and SSR-A = 0.79). Comparing the four marker systems to each other, AFLP and SSR-A were correlated best (r = 0.57). All other comparisons revealed rather low correlation coefficients in the Mantel Z test. With twice as many markers cophenetic values increased to a very good fit for AFLPs (0.90) and SSRs (0.92).      

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