scholarly journals Molecular phylogenetic analysis of members of Chrysanthemum and its related genera in the tribe Anthemideae, the Asteraceae in East Asia on the basis of the internal transcribed spacer (ITS) region and the external transcribed spacer (ETS) region of nrDNA

2009 ◽  
Vol 4 (2) ◽  
pp. 25-36 ◽  
Author(s):  
Yu Masuda ◽  
Tomohisa Yukawa ◽  
Katsuhiko Kondo
Keyword(s):  
Phylogenetic Analysis ◽  
East Asia ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
External Transcribed Spacer ◽  
Molecular Phylogenetic

Infundibulicybe macrospora sp. nov., a new and noteworthy species from Himalayan moist temperate forests of Pakistan

Phytotaxa ◽  
2020 ◽  
Vol 452 (4) ◽  
pp. 268-277
Author(s):  
MUHAMMAD ALI ◽  
JUNAID KHAN ◽  
HIRA BASHIR ◽  
ABDUL REHMAN NIAZI ◽  
HASSAN SHER ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Temperate Forests ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Reddish Orange ◽  
Light Orange

Infundibulicybe macrospora, a new species, is described and illustrated from the Himalayan moist temperate forests of Pakistan. The new species can be differentiated from close allies by its reddish orange to light orange pileus, whitish lamellae and stipe, and larger and broader basidiospores. The new species is also supported by molecular phylogenetic analysis inferred from internal transcribed spacer (ITS) region.

A new species of Conocybe (Bolbitaceae) from Punjab, Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 402 (5) ◽  
pp. 251
Author(s):  
AIMAN IZHAR ◽  
HIRA BASHIR ◽  
ABDUL NASIR KHALID
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Evidence ◽  
Fruiting Bodies ◽  
Germ Pore ◽  
Central Disc ◽  
Molecular Phylogenetic ◽  
A New Species

Conocybe punjabensis sp. nov., a species belonging to the saprobic family Bolbitaceae, is illustrated and described from Pakistan based on morphology and molecular evidence. The species can be distinguished on the basis of medium-sized fruiting bodies, a pileus with a dark brown central disc, a fibrillose stipe, forked lamellae near pileus margin, ellipsoid angular basidiospores with an apical germ pore, cheilocystidia and pleurocystidia varying from catenulate, lecythiform to clavate, and lecythiform caulocystidia. Molecular phylogenetic analysis of the nuclear ribosomal ITS region also supports the recognition of this new species in Conocybe. Detailed descriptions, photographs, illustrations and comparison with allied taxa are given.

A new Indo-Malayan family of Grassatores (Arachnida : Opiliones : Laniatores)

2019 ◽  
Vol 33 (6) ◽  
pp. 892
Author(s):  
Adriano B. Kury ◽  
Abel Pérez-González ◽  
Daniel N. Proud
Keyword(s):  
Phylogenetic Analysis ◽  
East Asia ◽  
Male Genitalia ◽  
Broad Context ◽  
External Morphology ◽  
Molecular Phylogenetic Analysis ◽  
South East Asia ◽  
New Family ◽  
The Family ◽  
Molecular Phylogenetic

The laniatorean family Phalangodidae has been largely reduced to a core of Holarctic species; however, many taxa were never formally transferred to other families. We examined a group of harvestmen related to Beloniscus Thorell, 1891, from South-east Asia and determined the nature of the relationships of the ‘Beloniscus-like harvestmen’, herein described as the new family Beloniscidae, fam. nov., in the broad context of Laniatores. Based on a molecular phylogenetic analysis of a broad representative laniatorean Sanger-sequences dataset we found support for our taxonomic hypotheses that (1) inclusion of Beloniscidae, fam. nov. in Phalangodidae would render the family non-monophyletic, and (2) Beloniscidae, fam. nov. represents a lineage that is morphologically and genetically distinct from all other known laniatorean families. The new family Beloniscidae is endemic to South-east Asia and comprises 37 species in two new subfamilies: Beloniscinae, subfam. nov. and Buparinae, subfam. nov. Beloniscinae includes the genera Beloniscellus Roewer, 1931, Beloniscops Roewer, 1949, Belonisculus Roewer, 1923, Beloniscus (type genus) and Kendengus Roewer, 1949. Buparinae includes the genera Buparellus Roewer, 1949, Bupares Thorell, 1889 (type genus) and Buparomma Roewer, 1949. Members of Beloniscidae are relatively homogeneous in their external morphology, but are recognised by well marked genitalic features. Male genitalia are described for exemplar species, and the morphology is compared with that of Epedanidae. Names are given to two new genitalic structures: sella curulis and pseudocalyx. http://zoobank.org/urn:lsid:zoobank.org:act:540BCE49-6F2E-4372-BFD4-D3C6068F045D http://zoobank.org/urn:lsid:zoobank.org:act:FE8B011B-AA6D-4E6C-A321-123542A0563F http://zoobank.org/urn:lsid:zoobank.org:act:E4A559CD-170A-40F2-924A-541C22974344

scholarly journals Ectomycorrhizae of Tomentella badia: description and molecular identification

2013 ◽  
Vol 48 (2) ◽  
pp. 155-171 ◽  
Author(s):  
Andrea Binder ◽  
Derek Peršoh ◽  
Nourou S. Yorou ◽  
Rita Verma ◽  
Claus Bässler ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Picea Abies ◽  
Molecular Identification ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Functional Characteristics ◽  
Molecular Phylogenetic ◽  
Dark Blue

Species within the genera <em>Tomentella </em>are among the most important ECM in forests. However, our knowledge about their functional characteristics is still rather limited. The ectomycorrhizae of <em>Tomentella badia </em>on <em>Picea abies </em>are described here in detail and compared to the non-identified ECM <em>Piceirhiza obscura</em>. A pseudoparenchymatous mantle formed by epidermoid cells is covered by heaps of epidermoid cells. This mantle type is regarded as a new one and designated as mantle type R. Many cells filled with dark blue contents and/or blue granules, together with clampless hyphae, are distinct characters of these ectomycorrhizae. Molecular-phylogenetic analysis of the ITS region was used for identification.

Inocybe pakistanensis, a new species in section Rimosae s. str. from Pakistan

Phytotaxa ◽  
2018 ◽  
Vol 348 (4) ◽  
pp. 279 ◽  
Author(s):  
ZIA ULLAH ◽  
SANA JABEEN ◽  
HABIB AHMAD ◽  
ABDUL NASIR KHALID
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Morphological Characters ◽  
Nuclear Ribosomal Dna ◽  
Molecular Phylogenetic Analysis ◽  
Its Regions ◽  
Molecular Phylogenetic ◽  
A New Species

Inocybe pakistanensis is described and illustrated as a new species based on morphological characters and molecular phylogenetic analysis of nuclear ribosomal DNA including the internal transcribed spacer (ITS) regions along with larger subunit (LSU). The distinctive basidiomata have a highly rimose and fibrillose golden brown pileus with a reddish brown, prominent umbo; ellipsoid to amygdaliform, slightly phasoeliform smooth basidiospores; and clamped septa in all the tissues. Molecular phylogenetic analysis supports the placement of I. pakistanensis in section Rimosae s. str.

scholarly journals The ITS region provides a reliable DNA barcode for identifying reishi/lingzhi (Ganoderma) from herbal supplements

2020 ◽  
Author(s):  
Tess Gunnels ◽  
Matthew Creswell ◽  
Janis McFerrin ◽  
Justen B. Whittall
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Analyses ◽  
Dna Barcode ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Herbal Supplement ◽  
Herbal Supplements ◽  
Expected Size Range ◽  
Molecular Phylogenetic ◽  
Species Authentication

AbstractThe dietary supplement industry is a growing enterprise, valued at over $100 billion by 2025 yet, a recent study revealed that up to 60% of herbal supplements may have substituted ingredients not listed on their labels, some with harmful contaminants. Substituted ingredients make rigorous quality control testing a necessary aspect in the production of supplements. Traditionally, species have been verified morphologically or biochemically, but this is not possible for all species if the identifying characteristics are lost in the processing of the material. One approach to validating plant and fungal ingredients in herbal supplements is through DNA barcoding complemented with a molecular phylogenetic analysis. This method provides an efficient, objective, rigorous and repeatable method for species identification. We employed a molecular phylogenetic analysis for species authentication of the commonly used fungal supplement, reishi (Ganoderma lingzhi), by amplifying and sequencing the nuclear ribosomal internal transcribed spacer regions (ITS) with genus-specific primers. PCR of six powdered samples and one dried sample sold as G. lucidum representing independent suppliers produced single, strong amplification products in the expected size-range for Ganoderma. Both best-hit BLAST and molecular phylogenetic analyses using a reference panel assembled from Genbank clearly identified the predominant fungal DNA was G. lingzhi in all seven herbal supplements. We detected variation in ITS among our samples, but all samples still fall within a large clade of G. lingzhi. ITS is a successful and cost-effective method for DNA-based species authentication that could be used in the herbal supplement industry for this and other fungal and plant species that are otherwise difficult to identify.

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