scholarly journals Phylogenetic analysis of Mexican and Central American Pinguicula (Lentibulariaceae) based on internal transcribed spacer (ITS) sequence

2007 ◽  
Vol 2 (2) ◽  
pp. 67-77 ◽  
Author(s):  
Hiro Shimai ◽  
Katsuhiko Kondo
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Central American ◽  
Its Sequence

scholarly journals First confirmed report on Fusarium sporotrichioides on Pinus ponderosa var. jeffreyi in Slovakia

2016 ◽  
Vol 52 (No. 4) ◽  
pp. 250-253 ◽  
Author(s):  
Ivanová Helena ◽  
Hrehová Ľudmila ◽  
Pristaš Peter
Keyword(s):  
Phylogenetic Analysis ◽  
Pinus Ponderosa ◽  
Internal Transcribed Spacer ◽  
Potato Dextrose Agar ◽  
Its Sequences ◽  
Its Sequence ◽  
Fusarium Sporotrichioides ◽  
Sequence Comparisons ◽  
Red Pigments ◽  
Petri Dishes

During 2014–2015, samples from ten 20–25 years old pine (Pinus ponderosa var. jeffreyi) trees showing wilt symptoms were collected from the Arborétum Mlyňany park. A disease was observed on 20% of the trees. The first symptoms are wilting, stunting, chlorosis, and discolouration of needles, which turned yellow on affected twigs, then red and finally they fell off. Isolations of the pathogen were done from the discoloured tissues of needles (twenty samples from each tree) on Potato Dextrose Agar. Colonies of the fungus (3–4 Petri dishes from each tree) were initially aerial, white or slightly violet, but with age they became red and red pigments were produced in agar. The observed micromorphological characteristics of the fungus, such as presence of simple and proliferating conidiophores with polyphialides, microconidia, macroconidial shape, and chlamydospore presence matched the description of Fusarium sporotrichioides. The identity of the fungus was confirmed by phylogenetic analysis of internal transcribed spacer (ITS) sequences. Sequence comparisons placed the fungus to the species F. sporotrichioides with similarity of 99.6% at the ITS sequence level.

Genetic diversity of Peucedanum japonicum using Internal Transcribed Spacer (ITS) sequence

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
YH Kim ◽  
JA Ryuk ◽  
BS Ko ◽  
JW Lee ◽  
SE Oh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Internal Transcribed Spacer ◽  
Its Sequence

Higher-level relationships among the eucalypts are resolved by ITS-sequence data

2002 ◽  
Vol 15 (1) ◽  
pp. 49 ◽  
Author(s):  
Dorothy A. Steane ◽  
Dean Nicolle ◽  
Gay E. McKinnon ◽  
René E. Vaillancourt ◽  
Brad M. Potts
Keyword(s):  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Its Region ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Its Sequence ◽  
Phylogenetic Groups ◽  
Single Section ◽  
Western Australian ◽  
Genus Eucalyptus

This expanded survey of ITS sequences represents the largest analysis of molecular data ever attempted on Eucalyptus. Sequences of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA were included in an analysis of 90 species of Eucalyptus s.s. and 28 species representing eight other genera (Allosyncarpia, Angophora, Arillastrum, Corymbia, Eucalyptopsis, Stockwellia, Lophostemon and Metrosideros). The results of the study indicate that Angophora and Corymbia form a well-supported clade that is highly differentiated from Eucalyptus s.s. Corymbia species are divided between two clades, one of which may be the sister to Angophora. Allosyncarpia, Arillastrum, Eucalyptopsis and ‘Stockwellia’ are also highly differentiated from Eucalyptus s.s. If the genus Eucalyptus is to be expanded to include Angophora and Corymbia(sensu Brooker 2000), ITS data suggest that Allosyncarpia, Eucalyptopsis, ‘Stockwellia’ and potentially Arillastrum should also be included in Eucalyptus s.l. The ITS data suggest that subg. Symphyomyrtus is paraphyletic and that subg. Minutifructus should be included within it. Within subg.Symphyomyrtus, only sect. Maidenaria appears to be monophyletic. Sections Adnataria and Dumaria are probably monophyletic; sections Exsertaria and Latoangulatae are very close and probably should be combined in a single section. Section Bisectae is polyphyletic and is divided into two distinct lineages. The phylogenetic groups depicted by ITS data are consistent with the frequency of natural inter-specific hybridisations as well as data from controlled crosses within subgenus Symphyomyrtus. The ITS data illustrate that subg. Idiogenes and western Australian monocalypts are early evolutionary lines relative to E. diversifolia, E. rubiginosa (monotypic subg. Primitiva) and the eastern monocalypts and that subg. Primitiva should be sunk into subg. Eucalyptus. Subgenus Eudesmia may be monophyletic, grouping with subgenera Idiogenes and Eucalyptus. Further work is required to confirm the phylogenetic positions of the monotypic subgenera Alveolata, Cruciformes, Acerosae and Cuboidea.

scholarly journals WildTermitomycesSpecies Collected from Ondo and Ekiti States Are More Related to African Species as Revealed by ITS Region of rDNA

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Victor Olusegun Oyetayo
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Tree ◽  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Its Sequence ◽  
African Countries ◽  
African Species ◽  
Its Sequence Analysis ◽  
Degree Of Similarity

Molecular identification of eighteenTermitomycesspecies collected from two states, Ondo and Ekiti in Nigeria was carried out using the internal transcribed spacer (ITS) region. The amplicons obtained from rDNA ofTermitomycesspecies were compared with existing sequences in the NCBI GenBank. The results of the ITS sequence analysis discriminated between all theTermitomycesspecies (obtained from Ondo and Ekiti States) andTermitomycessp. sequences obtained from NCBI GenBank. The degree of similarity of T1 to T18 to gene ofTermitomycessp. obtained from NCBI ranges between 82 and 99 percent.Termitomycesspecies from Garbon with ascension number AF321374 was the closest relative of T1 to T18 except T12 that has T. eurhizus and T. striatus as the closet relative. Phylogenetic tree generated with ITS sequences obtained from NCBI GenBank data revealed that T1 to T18 are more related toTermitomycesspecies indigenous to African countries such as Senegal, Congo, and Gabon.

Pseudodactylaria brevis sp. nov. from Thailand confirms the status of Pseudodactylariaceae

Phytotaxa ◽  
2018 ◽  
Vol 369 (4) ◽  
pp. 241 ◽  
Author(s):  
CHUAN-GEN LIN ◽  
ERIC H. C. MCKENZIE ◽  
DARBHE J. BHAT ◽  
JIAN-KUI LIU ◽  
KEVIN D. HYDE ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Sequence Data ◽  
Taxonomic Status ◽  
Its Sequence ◽  
Morphological Comparison ◽  
Phylogenetic Placement ◽  
Data Analyses ◽  
The Status ◽  
A New Species

A new species, Pseudodactylaria brevis, is described, illustrated and compared with other Pseudodactylaria and Dactylaria-like taxa. Evidence for the new species is provided by morphological comparison and sequence data analyses. Pseudodactylaria brevis can be distinguished from other Pseudodactylaria and Dactylaria-like species by its short hyaline conidiophores and fusiform, 1-septate hyaline conidia. Phylogenetic analysis of LSU and ITS sequence data was carried out to determine the phylogenetic placement of the species and confirm the taxonomic status of Pseudodactylariaceae.

Group I Intron Versus its Sequences in Phylogeny of Cetrarioid Lichens

1999 ◽  
Vol 31 (5) ◽  
pp. 441-449 ◽  
Author(s):  
Arne Thell
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Trees ◽  
Group I Intron ◽  
Ribosomal Genes ◽  
Its Sequences ◽  
Its Sequence ◽  
Ribosomal Rna Gene ◽  
18S Ribosomal Rna Gene ◽  
Group I

AbstractPhylogenetic trees based on group I intron sequences and on internal transcribed spacer (ITS) sequences of mycobiont ribosomal genes were calculated and compared. Eight cetrarioid and four non-cetrarioid species of the Parmeliaceae were compared. The phylogeny based on group I intron sequences is partly congruent with the ITS sequence phylogeny. Group I intron sequences are presumably less informative for infragenic studies. The introns have a length of 214–233 nucleotides, and differ at up to 33% of the bases between species. All introns analysed are located between the positions 1516 and 1517 of the fungal 18S ribosomal RNA gene. Cetrarioid lichens form a non-homogeneous group within the Parmeliaceae according to both group I intron and ITS sequences.

scholarly journals Three new Russula species in sect. Ingratae (Russulales, Basidiomycota) from southern China

MycoKeys ◽  
2021 ◽  
Vol 84 ◽  
pp. 103-139
Author(s):  
Guo-Jie Li ◽  
Shou-Mian Li ◽  
Bart Buyck ◽  
Shi-Yi Zhao ◽  
Xue-Jiao Xie ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Southern China ◽  
Its Region ◽  
Line Drawings ◽  
Phylogenetic Data ◽  
Three New Species ◽  
First Time

Three new species of Russulasection Ingratae, found in Guizhou and Jiangsu Provinces, southern China, are proposed: R. straminella, R. subpectinatoides and R. succinea. Photographs, line drawings and detailed morphological descriptions for these species are provided with comparisons against closely-related taxa. Phylogenetic analysis of the internal transcribed spacer (ITS) region supported the recognition of these specimens as new species. Additionally, R. indocatillus is reported for the first time from China and morphological and phylogenetic data are provided for the Chinese specimens.

scholarly journals Diversity of Ascomycetes Mushroom in Para Rubber Plantation, Thailand

2021 ◽  
Author(s):  
Saowapha Surawut ◽  
Sorasak Nak-aim ◽  
Chutapa Kunsook ◽  
Laddawan Kamhaengkul ◽  
Pornpimon Kanjanavas ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Genetic Relatedness ◽  
Sequence Similarity ◽  
Its Region ◽  
Bioactive Compound ◽  
Rubber Plantation ◽  
Mushroom Species ◽  
Reference Strains

Abstract Ascomycetes mushrooms are fungi that produce ascospores in asci and some with perithecia. Not only they have a role of decomposer in ecology but also produced some bioactive compound, anti-microbial activity, and cytotoxicity. This study aims to explore the diversity of ascomycetes mushroom species in para rubber plantations and to identify them by morphological and sequence analysis of the internal transcribed spacer (ITS) region. The results found ascomycetes mushroom consist of Trichoderma pezizoides (RP1, % identity 98.79, DQ835513.1), Daldinia eschscholtzii (RP2, % identity 100, MN310384.1), Cookeina sulcipes (RP3, % identity 98.44, KY094620.1), Cookeina garethjonesii (RP4, % identity 99.06, KY094622.1), Cookeina tricholoma (RP5, % identity 100, KY094619.1) and Xylaria terricola (RP6, % identity 88.42, MF577038.1). Most of the ascomycetes in this study have previously been described in Thailand except Xylaria terricola. Additionally, phylogenetic analysis of ascomycetes mushroom showed high genetic relatedness with reference strains. Therefore, the sequence similarity and phylogenetic analysis confirmed the identity of six ascomycetes mushroom species, and further study of bioactive compound from these mushrooms may be investigated for other applications.

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