scholarly journals Fluorescent band pattern of chromosomes in Ephedra americana var. andina, Ephedraceae

2016 ◽  
Vol 11 (2) ◽  
pp. 27-30 ◽  
Author(s):  
Masahiro Hizume ◽  
Kazuo Tominaga
Keyword(s):  
Band Pattern ◽  
Fluorescent Band

EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SUNITA BORDE ◽  
ASAWARI FARTADE ◽  
AMOL THOSAR ◽  
RAHUL KHAWAL
Keyword(s):  
Fresh Water ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Band Pattern ◽  
Genomic Variation ◽  
Polymorphic Band ◽  
Rapd Profile ◽  
Band Size ◽  
Pcr Product ◽  
The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

Hexa-band pattern reconfigurable antenna with defected ground plane

2021 ◽  
pp. 1-15
Author(s):  
Ghanshyam Singh ◽  
Binod Kumar Kanaujia ◽  
Vijay Kumar Pandey ◽  
Deepak Gangwar ◽  
Sachin Kumar
Keyword(s):  
Ground Plane ◽  
Band Pattern ◽  
Reconfigurable Antenna ◽  
Defected Ground Plane

scholarly journals Auxiliary Method for Clonal Identification ofStaphylococcus aureusby Protein Band Pattern of Released Proteins on SDS-Polyacrylamide Gel

1995 ◽  
Vol 39 (8) ◽  
pp. 615-617 ◽  
Author(s):  
Keiko Seki ◽  
Junji Sakurada ◽  
Miyo Murai ◽  
Akemi Usui ◽  
Hee Kyong Seong ◽  
...  
Keyword(s):  
Protein Band ◽  
Polyacrylamide Gel ◽  
Band Pattern ◽  
Clonal Identification

Variation in the electrophoretic band pattern of tuber proteins from somaclones of potato

1986 ◽  
Vol 106 (2) ◽  
pp. 427-428 ◽  
Author(s):  
D. B. Smith
Keyword(s):  
Molecular Weight ◽  
Band Pattern ◽  
Polyacrylamide Gels ◽  
Potato Varieties ◽  
Electrophoretic Band ◽  
Native Proteins ◽  
Solatium Tuberosum ◽  
Specific Variation

The soluble tuber proteins of potato (Solatium tuberosum) may be separated by electrophoresis on the basis of charge or molecular weight (Stegemann & Schnick, 1982; Maier & Wagner, 1981: Park et al. 1983). Considerable cultivar specific variation exists in the band patterns of these proteins and separation of native proteins in 6% polyacrylamide gels at pH 7·9 has been used as the basis of characterizing cultivars for the Index of European Potato Varieties (Stegemann & Schnick, 1982).

scholarly journals Residual amounts of glycoprotein Ib concomitant with near-absence of glycoprotein IX in platelets of Bernard-Soulier patients

Blood ◽  
1988 ◽  
Vol 72 (3) ◽  
pp. 1086-1088 ◽  
Author(s):  
J Drouin ◽  
JL McGregor ◽  
S Parmentier ◽  
CA Izaguirre ◽  
KJ Clemetson
Keyword(s):  
Normal Level ◽  
Polyclonal Antibodies ◽  
Band Pattern ◽  
Platelet Membrane ◽  
Membrane Glycoprotein ◽  
Glycoprotein Ib ◽  
Gene Deletions ◽  
Immunoblot Assay ◽  
Platelet Membrane Glycoprotein ◽  
Bernard Soulier Syndrome

A study of the Bernard-Soulier syndrome in two unrelated families using different polyclonal antibodies in a sensitive immunoblot assay showed residual amounts of platelet membrane glycoprotein (GP) lb in the eight homozygotes, as well as the near-absence of GPlb beta and GPIX. The eight heterozygotes studied showed a double band pattern for GPlb and about half the normal level of GPlb beta and GPIX. Therefore, we conclude that the Bernard-Soulier syndrome is heterogeneous and is probably not due to gene deletions.

scholarly journals Neuronal N-acetyl-β-D-glucosaminidase. Evidence for its biosynthesis in vitro

1976 ◽  
Vol 158 (3) ◽  
pp. 513-527 ◽  
Author(s):  
J A Khawaja ◽  
O Z Sellinger
Keyword(s):  
Concanavalin A ◽  
Microsomal Fraction ◽  
Deae Cellulose ◽  
Neuronal Cell ◽  
Amino Acid Mixture ◽  
Acid Mixture ◽  
Freezing And Thawing ◽  
Cellulose Acetate Electrophoresis ◽  
Fluorescent Band

Neuronal cell bodies, isolated in bulk from 8-day-old rat cerebral cortices, were incubated in the presence of a 3H-labelled amino acid mixture, and subcellular fractions isolated by differential centrifugation. The particulate fractions were frozen/thawed in 0.20 M-sucrose/0.1 M-KCl [Selling et al. (1973) Biochim. Biophys. Acta 315, 128-146] and the profiles of acid-insoluble radioactivity and N-acetyl-beta-D-glucosaminidase (glucosaminidase) activity compared in the resulting non-sedimentable fractions by DEAE-cellulose chromatography and cellulose acetate electrophoresis. Radioactivity and glucosaminidase activity co-migrated to a significant extent. Electrophoresis revealed that after 1 min of incubation 42% of the radioactivity of the non-sedimentable microsomal fraction after freezing and thawing co-migrated with an intensely fluorescent band of glucosaminidase activity. Since the pellet fraction obtained on freezing/thawing the microsomal fraction contained up to 75% of the RNA, 95% of the radioactivity and 45% of the glucosaminidase, a detailed study of the association between its radioactivity and nascent glucosaminidase activity was undertaken. After 1 and 2 min of incubation, followed by centrifugation of the microsomal pellet on 35-60% (w/v) sucrose density gradients, radioactivity and glucosaminidase activity exhibited parallel profiles in the region of heavy polyribosomes and at the top of the gradient which contains spontaneously released nascent polypeptide chains. DEAE-cellulose chromatography of these chains revealed glucosaminidase A to be the principal nascent glucosaminidase component, with glucosaminidases B and C as minor peaks. After 2 min of incubation, all of the glucosaminidase components appeared labelled, and glucosaminidase A exhibited two distinct sub-components. The pattern of glucosaminidase labelling in the soluble and microsomal fractions suggested that newly formed glucosaminidase molecules traverse both the cellular sap and the lumen of the endoplasmic reticulum. Only glucosaminidase A reacted specifically with concanavalin A and radioactive glucosaminidase A could be successfully regenerated by treatment with alpha-methyl-D-glucoside. Glucosaminidase A and a substantial portion of the radioactivity associating with it could be readily converted into glucosaminidase B by re-chromatography on DEAE-cellulose and by reaction of the concanavalin A-glucosaminidase A complex with methyl glucosides.

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