scholarly journals Skrining Fitokimia dan Uji Antioksidan Ekstrak Biji Kopi Sangrai Jenis Arabika (Coffea arabica) Asal Wamena dan Moanemani, Papua

2019 ◽  
Vol 11 (2) ◽  
pp. 103-109
Author(s):  
Septiani Mangiwa ◽  
Agnes Eri Maryuni
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phytochemical Analysis ◽  
Coffee Bean ◽  
Roasted Coffee ◽  
Phytochemical Content ◽  
Phytochemical Investigation ◽  
Coffee Beans ◽  
Dpph Method ◽  
Roasted Coffee Beans

Coffee bean are rich of secondary metabolits that able to inhibit free radical compounds. This antioxidant activity may reduce many diseases correlated with it. The aims of this study were to determined the phytochemical content and antioxidant activity of roasted coffee bean from Wamena and Moenemani regency, Papua. Roasted coffee beans were extracted by maceration for 24 hr with methanol. Harborne standard method was used for the phytochemical analysis  and DPPH assay was used to  determine the antioxidant activity. IC50 was measured by spectrophotometric assay using spectrophotometer Uv-Vis at 517 nm wavelenghth. Result showed that both Arabica roasted coffee beans from Wamena and Moanemany had the capacity to inhibit free radical  at 61,71% and 69,7% with IC50 at  107,97 and 100,91 ppm, respectively . Phytochemical investigation revealed that the bioactive compounds from Moanemani and Wamena coffee beans were similar, which composed of alkaloids, flavonoids, terpenoids, saponins, and tanins. In conclusion, the methanolic extract of roasted Arabica coffee beans from Wamena and Moanemani can be used as the source of natural antioxidant.Keywords: Arabica roasted coffee beans; phytochemical; antioxidant; DPPH method.

scholarly journals PRELIMINARY PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF ARAUCARIA COLUMNARIS BARK PEEL AND COSMOS SULPHUREUS FLOWERS

2017 ◽  
Vol 9 (4) ◽  
pp. 96 ◽  
Author(s):  
Krishma M. Jadav ◽  
K. N. Ninge Gowda
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Phytochemical Composition ◽  
Dpph Method ◽  
In Vitro Antioxidant Activity

Objective: Four different extracts of Araucaria columnaris (bark peel) and Cosmos sulphureus (flowers) were screened for their phytochemical composition, and free radical scavenging activities.Methods: DPPH method was used to test the antioxidant activity for extracts.Results: Among the different extracts tested, the methanol extract of both the plant species showed significant radical scavenging activities. Phytochemical analysis of the extracts revealed that the radical scavenging activities might be due to the presence of flavonoids, tannins and phenolic compounds.Conclusion: The results obtained suggest that Araucaria columnaris (bark peel) and Cosmos sulphureus(flowers) could be exploited in the treatment of various diseases like cancer, cardiovascular diseases and infection diseases.  

scholarly journals Optimization of Coffee Liqueur Manufacturing Process using Caffeine Content

2019 ◽  
Vol 9 (1) ◽  
pp. 430-433
Keyword(s):  
Alcoholic Beverage ◽  
The Other ◽  
Coffee Bean ◽  
Roasted Coffee ◽  
Caffeine Concentration ◽  
Caffeine Content ◽  
Other Hand ◽  
Coffee Beans ◽  
Roasted Coffee Beans

Coffee liqueur is an alcoholic beverage made by soaking coffee in spirit. Though coffee liqueur is one of the wellknown liqueur, few research was made so far. In this research, coffee liqueur was prepared by using different recipes involving liquors with different ethanol concentrations (20, 40, or 80%), and the soaking periods (14, 21, or 30 days) of the coffee liqueur ingredients (roasted coffee beans, coffee bean powder and other ingredients). Volatile and semi-volatile compounds are important for the organoleptic quality of roasted coffee beans. However, the amounts of these compounds in roasted coffee beans are more dependent on roasting conditions than those of non-volatiles. Furthermore, volatiles are more easily lost during storage. On the other hand, caffeine, a well-known compound of coffee is an intrinsic non-volatile chemical, and the caffeine is present in larger than other potential indicator components in coffee beans, ensuring the quantitative consistency of the coffee extracts in the liqueur. Caffeine was quantified from the prepared coffee liqueurs by HPLC after method validation. The average caffeine content of all 54 analyses (6 analyses per sample) was 2081.2 μg/mL. The highest caffeine concentration 2793 μg/mL was acquired in the sample prepared by 80% ethanol liquor. A soaking period of 14 days, was considered inadequate for sufficient caffeine extraction regardless of liquor alcohol content. Caffeine concentration peaked after soaking coffees and other ingredients for 21 days at 80% ethanol concentration. On the other hand, the most consistent caffeine concentrations in liqueurs were obtained by soaking for 21 days in 40% ethanol (RSD 7.8%), thus it could be the optimum coffee liqueur recipe. The consistency of coffee liqueur quality was assured using caffeine content.

scholarly journals Comparative Study of Antioxidant Property of Coffee and Its Additives

2021 ◽  
pp. 89-96
Author(s):  
Deepti Bajaj ◽  
Suhas Ballal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Comparative Study ◽  
Antioxidant Properties ◽  
Group Analysis ◽  
Antioxidant Property ◽  
Roasted Coffee ◽  
Instant Coffee ◽  
Coffee Beans ◽  
Roasted Coffee Beans

Coffee, one of the most commonly consumed beverages is a very rich source of antioxidants alongside various other health benefits. The roasted beans of coffee are the seed of berry obtained from coffea species. These roasted coffee beans are utilized to prepare coffee. India is today producer of 16 unique varieties of coffee most of which originate from southern India. The goodness of coffee and Ganoderma can do wonders to human health. This study is aimed to have an analysis of antioxidant properties of instant coffee, filter coffee, coffee with Ganoderma extracts and ginger coffee by estimating the Ascorbic Acid equivalents (AAE). The essence of the study is the presence of additions and the antioxidant activity of the coffee samples in their presence. The two infusions used in the study are extracts of Ganoderma and ginger. The study also aims to have a inter group analysis of antioxidant properties of all the samples. The study was conducted using basic colorimetric techniques.

scholarly journals EVALUASI MUTU KOPI ROBUSTA DI KECAMATAN KARE KABUPATEN MADIUN JAWA TIMUR

Pro Food ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 729
Author(s):  
Choiroel Anam ◽  
Eny Muzayana ◽  
Rohmat Priya Atmaja ◽  
Didik Purnomo
Keyword(s):  
Moisture Content ◽  
Microbiological Quality ◽  
Coffee Bean ◽  
Roasted Coffee ◽  
Physical Quality ◽  
Caffeine Content ◽  
Physical Chemical ◽  
Coffee Beans ◽  
Roasted Coffee Beans

ABSTRACT   Coffee is a leading commodity in Madiun Regency, East Java, the area of coffee plantations in 2018  is 1,432 Ha with an average productivity of 769.63 kg / ha /year.  This study aims to provide information on the physical, chemical, and microbiological quality characteristics of rice coffee, roasted coffee beans and evaluate the primary processing procedures for Mugi Lestari Farmer Group's coffee in Kare Village, Kare District, Madiun Regency. Evaluation of the quality of coffee rice is based on general and specific quality equirements according to SNI 01-2907: 2008. Organoleptic and chemical analysis of rice coffee and roasted coffee beans was carried out to see the state (smell, taste), moisture content, ash content, ash alkalinity, caffeine content, sugar content (reducing), insoluble solids, metal contaminants (Pb and Cu). Assessment of the physical quality of coffee beans shows that the robusta rice coffee produced at Poktan Mugi Lestari has quality according to SNI 01-2907-2008, especially for the parameters of the presence of insects, moisture content, impurities, and no found odor/mold.  Physical quality of roasted coffee beans from Poktan Mugi Lestari when compared to SNI standards has a normal condition, both in terms of smell, taste, and appearance. Chemical parameters of roasted coffee bean samples are also in accordance with the general requirements for roasted coffee (SNI 01-2983-1992). The number of bacteria and molds is less than the maximum limit set. Assessment of physical, chemical, and microbiological quality of packaged coffee powder has a value in accordance with the SNI quality requirements, both for caffeine content, the amount of mold, and bacteria (ALT value), and water content. The primary coffee processing process at Poktan Mugi Lestari has been able to produce rice coffee in accordance with SNI requirements.   Keywords: coffee, robusta, quality, kare madiun   ABSTRAK               Kopi merupakan komoditas unggulan di Kabupaten madiun Jawa Timur, Luas areal perkebunan kopi tahun 2018 di Kabupaten Madiun  1.432 Ha dengan produktivitas rata rata 769,63 kg/ha/tahun. Penelitian ini bertujuan memberikan informasi karakteristik mutu fisik, kimiawi, dan mikrobiologis dari kopi beras, kopi biji sangrai dan mengevaluasi prosedur pengolahan primer kopi Kelompok Tani Mugi Lestari di desa Kare, Kecamatan Kare, Kab Madiun, agar dihasilkan produk dengan mutu yang baik. Evaluasi mutu kopi beras didasarkan pada syarat mutu umum dan khusus sesuai SNI 01-2907:2008. Analisa organoleptik dan kimiawi kopi beras dan kopi biji sangrai dilakukan untuk melihat keadaan (bau, rasa), kadar air, kadar abu, kealkalian abu, kadar kafein, kadar gula (pereduksi), padatan tak larut dalam air, cemaran logam (Pb dan Cu). Penilaian mutu fisik biji kopi diketahui bahwa kopi beras robusta yang dihasilkan di Poktan Mugi Lestari memiliki mutu sesuai dengan SNI 01-2907-2008, khususnya untuk parameter keberadaan serangga, nilai kadar air, pengotor, dan tidak ditemukannya bau busuk/kapang. Keadaan mutu fisik biji kopi sangrai dari Poktan Mugi Lestari jika dibandingan dengan SNI 01-2983-1992 memiliki keadaan yang normal, baik dalam hal bau, rasa, dan penampakan. Parameter kimiawi biji kopi sangrai dalam penelitian juga sesuai dengan syarat umum kopi sangrai (SNI 01-2983-1992). Jumlah bakteri dan kapang kurang dari batas maksimum yang ditetapkan. Penilaian mutu fisik, kimiawi, dan mikrobiologis bubuk kopi yang dikemas memiliki nilai sesuai dengan persyaratan mutu SNI, baik untuk kadar kafein, jumlah kapang, dan bakteri (nilai ALT), dan kadar air. Proses pengolahan primer kopi di Poktan Mugi Lestari sudah mampu menghasilkan kopi beras yang sesuai dengan persyaratan SNI.     Kata kunci: Kopi, Robusta, Mutu, Kare Madiun

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF PURNAJIWA FRUIT EXTRACT (Euchresta horsfieldii (Lesch.) Benn.)

2020 ◽  
Vol 8 (1) ◽  
pp. 31
Author(s):  
Romulia Trielva Apriliani ◽  
I Gede Putu Wirawan ◽  
Wayan Adiartayasa
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Chemical Compound ◽  
Radical Reaction ◽  
Phytochemical Analysis ◽  
Free Radical Reaction ◽  
Fruit Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method

Antioxidant is a chemical compound that could give one or more electrons to inhibit the free radical reaction. One of the plants that potentially produce antioxidants is purnajiwa (Euchresta horsfieldii (Lesch.) Benn.). The research was conducted using the fruit of purnajiwa. The purpose of this study is to know antioxidant activity from purnajiwa fruit using the DPPH method and measured by UV-Vis spectrophotometry. The extract of purnajiwa fruit gained by the maceration method using 96% ethanol solvent. The results of phytochemistry screening found out that the extract contains flavonoid, alkaloid, saponin, tannin, and terpenoid. According to flavonoid testing using quercetin, the results obtained from purnajiwa extract contained flavonoid of 1,651 mg QE/g. The activity test of antioxidants quantitatively by DPPH using UV-Vis spectrophotometry found out that purnajiwa extract has IC50 value of 57,28 ppm so that purnajiwa extract has strong antioxidant activity that could be used as natural antioxidant resources.

scholarly journals Comparison of the Antioxidant Activities and Volatile Compounds of Coffee Beans Obtained Using Digestive Bio-Processing (Elephant Dung Coffee) and Commonly Known Processing Methods

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 408
Author(s):  
Mesfin Haile ◽  
Hyung Min Bae ◽  
Won Hee Kang
Keyword(s):  
Antioxidant Activity ◽  
Volatile Compounds ◽  
Green Coffee ◽  
Roasted Coffee ◽  
Green Beans ◽  
Processing Methods ◽  
Green Coffee Beans ◽  
Coffee Beans ◽  
Roasted Coffee Beans ◽  
Elephant Dung

There are different types of coffee processing methods. The wet (WP) and dry processing (DP) methods are widely practiced in different parts of coffee-growing countries. There is also a digestive bioprocessing method in which the most expensive coffee is produced. The elephant dung coffee is produced using the digestive bioprocessing method. In the present experiment, the antioxidant activity and volatile compounds of coffee that have been processed using different methods were compared. The antioxidant activity, total phenolic content (TPC), total flavonoid content (TFC), and total tannin content (TTC) of green coffee beans from all treatments were higher as compared to roasted coffee beans. Regarding the green coffee beans, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of elephant dung coffee beans was higher as compared to that of the DP and WP coffee beans. The green coffee beans had higher DPPH activity and ferric reducing antioxidant power (FRAP) value compared to the roasted coffee beans. The green beans of elephant dung coffee had a high TPC than the beans obtained by WP and DP methods. TFC in elephant dung coffee in both green and roasted condition was improved in contrast to the beans processed using dry and wet methods. The elephant dung coffee had an increased TTC in comparison to the DP and WP coffee (green beans). About 37 volatile compounds of acids, alcohols, aldehydes, amide, esters, ethers, furans, furanones, ketones, phenols, pyrazines, pyridines, Heterocyclic N, and pyrroles functional classes have been found. Some of the most abundant volatile compounds detected in all treatments of coffee were 2-furanmethanol, acetic acid, 2-methylpyrazine, 2,6-dimethylpyrazine, pyridine, and 5-methylfurfural. Few volatile compounds have been detected only in elephant dung coffee. The principal component analysis (PCAs) was performed using the percentage of relative peak areas of the volatile compound classes and individual volatile compounds. This study will provide a better understanding of the impacts of processing methods on the antioxidants and volatile compounds of coffee.

scholarly journals Comparison of antioxidant activity between green and roasted coffee beans using molecular methods

2015 ◽  
Vol 12 (5) ◽  
pp. 7293-7302 ◽  
Author(s):  
ALEXANDROS PRIFTIS ◽  
DIMITRIOS STAGOS ◽  
KONSTANTINOS KONSTANTINOPOULOS ◽  
CHRISTINA TSITSIMPIKOU ◽  
DEMETRIOS A. SPANDIDOS ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Molecular Methods ◽  
Roasted Coffee ◽  
Coffee Beans ◽  
Roasted Coffee Beans

scholarly journals Chemical Analysis, Toxicity Study, and Free-Radical Scavenging and Iron-Binding Assays Involving Coffee (Coffea arabica) Extracts

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4169
Author(s):  
Nuntouchaporn Hutachok ◽  
Pimpisid Koonyosying ◽  
Tanachai Pankasemsuk ◽  
Pongsak Angkasith ◽  
Chaiwat Chumpun ◽  
...  
Keyword(s):  
Free Radical ◽  
Mononuclear Cells ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Chemical Compositions ◽  
Coffee Bean ◽  
Dependent Manner ◽  
Roasted Coffee ◽  
Instant Coffee ◽  
Radical Scavenging Properties

We aimed to analyze the chemical compositions in Arabica coffee bean extracts, assess the relevant antioxidant and iron-chelating activities in coffee extracts and instant coffee, and evaluate the toxicity in roasted coffee. Coffee beans were extracted using boiling, drip-filtered and espresso brewing methods. Certain phenolics were investigated including trigonelline, caffeic acid and their derivatives, gallic acid, epicatechin, chlorogenic acid (CGA) and their derivatives, p-coumaroylquinic acid, p-coumaroyl glucoside, the rutin and syringic acid that exist in green and roasted coffee extracts, along with dimethoxycinnamic acid, caffeoylarbutin and cymaroside that may be present in green coffee bean extracts. Different phytochemicals were also detected in all of the coffee extracts. Roasted coffee extracts and instant coffees exhibited free-radical scavenging properties in a dose-dependent manner, for which drip coffee was observed to be the most effective (p < 0.05). All coffee extracts, instant coffee varieties and CGA could effectively bind ferric ion in a concentration-dependent manner resulting in an iron-bound complex. Roasted coffee extracts were neither toxic to normal mononuclear cells nor breast cancer cells. The findings indicate that phenolics, particularly CGA, could effectively contribute to the iron-chelating and free-radical scavenging properties observed in coffee brews. Thus, coffee may possess high pharmacological value and could be utilized as a health beverage.

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