scholarly journals Identifikasi dan Fotodegradasi Pigmen Klorofil Rumput Laut Caulerpa racemosa (Forsskal) J.Agardh

2018 ◽  
Vol 4 (2) ◽  
pp. 47-53
Author(s):  
Lisiard Dimara ◽  
Helena Tuririday ◽  
Tien N.B. Yenusi
Keyword(s):  
Chlorophyll A ◽  
Uv Light ◽  
Black Spot ◽  
Light Sources ◽  
Light Radiation ◽  
Chlorophyll B ◽  
Caulerpa Racemosa ◽  
Green Spot ◽  
Tlc Plates ◽  
Layer Chromatography

Identification of chlorophyll compound of Caulerpa racemosa has been carried out using Thin Layer Chromatography (TLC) method. Four compound has been found namely xanthophll, chlorophyll b, chlorophyll a, and phaeophytin. Xanthophyll was identified with the yellow spot on TLC plates which the Rf is 0,46. Chlorophyll b is yellow green spot with Rf is 0,61, the Rf of Chlorophyll a is 0,69 with the color is blue green, whereas phaeophytin is indicated as a black spot with the Rf  0,77. Volpi and UV light sources were used in this study, the volpi light radiation was caused chlorophyll extract degradation about 80,87% at Qy of l 663 nm, and the UV light source caused 72,27%  degradation at l 663 nm.Key words: identification, photodegradation, chlorophyll, Caulerpa racemosa.

scholarly journals Komposisi Pigmen pada Ulva sp., Padina australis dan Hypnea sp. dari Pantai Tablolong Provinsi Nusa Tenggara Timur

2020 ◽  
Vol 23 (2) ◽  
pp. 225-233
Author(s):  
Martice Desi Pesang ◽  
James Ngginak ◽  
Alfred Gasper Onisimus Kase ◽  
Coni Lisandra Balle Bisilissin
Keyword(s):  
Content Analysis ◽  
Thin Layer ◽  
Chlorophyll A ◽  
Pigment Content ◽  
Chlorophyll B ◽  
Pigment Analysis ◽  
Chlorophyll Pigments ◽  
Qualitative And Quantitative ◽  
Time Period ◽  
Layer Chromatography

Indonesia has marine biological natural resources consisting of fish, seaweed, crabs and shrimp which are very potential. The purpose of this study was to determine the composition of pigments in Ulva sp (Chlorophyta), Padina australis (Paeophyta) and Hypnea sp (Rhodophyta) obtained from Tablolong waters. The method used in this research is descriptive qualitative and quantitative. Proof of pigment in samples using thin layer chromatography (TLC). The results of pigment analysis of Ulva sp obtained twelve color spots with identified pigment types consisting of chlorophyll a, chlorophyll b, xanthophyll, b-carotene and feofitin. The pigment content identified in Padina australis samples are chlorophyll b, feoforbid, xanthophyll, chlorophyll a, carotene and feofitin a. The types of pigments identified in Ulva sp include pigment chlorophyll a, chlorophyll b, carotene and feofitin from the total color spots formed. Based on the results of this study concluded that non-cultivated seaweed obtained from Tablolong waters contains carotenoid and chlorophyll pigments. Suggestions from this study are expected to do an analysis of pigment content analysis in the dry season time period and continued with antioxidant testing.  Indonesia memiliki Sumber Daya Alam hayati laut yang terdiri dari ikan, rumput laut, kepiting dan udang yang sangat potensial. Tujuan penelitian ini adalah untuk mengetahui komposisi pigmen pada Ulva sp (Chlorophyta), Padina australis (Paeophyta)  dan Hypnea sp (Rhodophyta)  yang diperoleh dari perairan Tablolong. Metode yang digunakan dalam penelitian ini adalah deskriptif kualitatif dan kuantitatif. Pembuktian pigmen pada sampel menggunakan Kromatografi lapis tipis (KLT). Hasil analisis pigmen dari Ulva sp diperoleh dua belas spot warna dengan jenis pigmen yang teridentifikasi terdiri dari klorofil a, klorofil b, xantofil, b-karoten dan feofitin. Kandungan pigmen yang teridentifikasi pada sampel Padina australis adalah klorofil b, feoforbid, xantofil, klorofil a, karoten dan feofitin a. Jenis pigmen yang teridentifikasi pada Ulva sp meliputi pigmen klorofil a, klorofil b, karoten dan feofitin dari total spot warna yang terbentuk. Berdasarkan hasil penelitian dapat disimpulkan bahwa rumput laut non budidaya yang diperoleh dari perairan Tablolong memiliki kandungan pigmen karotenoid dan klorofil. Saran dari penelitian ini diharapkan dilakukan peneltian analisis kandungan pigmen pada periode waktu musim kemarau serta dilanjutkan dengan uji antioksidan.

Stabilitas Ekstrak Kasar Klorofil-a dan b Rumput Laut Caulerpa racemosa (Forsskal) J. Agardh 1873 pada Suhu dan Lama Penyimpanan yang Berbeda

2021 ◽  
Vol 10 (2) ◽  
pp. 184-189
Author(s):  
Ihsan Mahfudh ◽  
Gunawan Widi Santosa ◽  
Rini Pramesti
Keyword(s):  
Chlorophyll A ◽  
Room Temperature ◽  
Ph Value ◽  
Photosynthetic Pigment ◽  
Laboratory Method ◽  
Cold Temperature ◽  
Chlorophyll B ◽  
Caulerpa Racemosa ◽  
Randomized Design ◽  
Green Seaweed

Caulerpa racemosa merupakan salah satu jenis rumput laut hijau (Chlorophyta) yang memiliki pigmen fotosintetik klorofil-a dan klorofil-b. Pigmen tersebut labil terhadap suhu dan cahaya. Tujuan penelitian ini adalah mengetahui pengaruh suhu dingin (9oC) dan suhu ruang (29oC) terhadap stabilitas ekstrak kasar pigmen klorofil dengan lama penyimpanan 7 hari. Metode yang digunakan pada penelitian adalah eksperimental laboratoris. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) faktorial dengan 2 faktor yaitu suhu (9oC, 29oC) dengan interval pengujian (1, 3, 5, 7 hari). Ekstraksi dilakukan dengan metode maserasi dengan pelarut ethanol 96%. Parameter yang diamati berupa konsentrasi klorofil-a, klorofil-b, kecerahan ekstrak dan pH. Hasil penelitian menunjukkan konsentrasi klorofil-a, klorofil-b, dan pH menurun sedangkan kecerahan ekstrak meningkat setelah disimpan selama 7 hari baik pada suhu dingin maupun suhu ruang. Penurunan tertinggi konsentrasi klorofil-a sebesar 1,32 mg/g, klorofil-b sebesar 2,09 mg/g dan nilai pH sebesar 6 pada penyimpanan suhu ruang, penyimpanan pada suhu dingin mengalami penurunan terendah dengan konsentrasi klorofil-a sebesar 1,42 mg/g, klorofil-b sebesar 2,21 mg/g dan nilai pH sebesar 6,2. Nilai kecerahan ekstrak mengalami kenaikan tertinggi pada penyimpanan suhu ruang sebesar 30,99, sedangan terendah pada suhu dingin sebesar 29,25. Caulerpa racemosa is a one kind of green seaweed (Chlorophyta) which has photosynthetic pigment chlorophyll-a and chlorophyll-b. The pigment is unstable with temperature and light. The purpose of this research is to find out the effect of cold temperature (9oC) and room temperature (29oC) on the stability of crude extract of C. racemosa chlorophyll pigment with a storage time of 7 days. This research was using an experimental laboratory method. The research design was used factorial Completely Randomized Design (CRD) with 2 factors, temperature (9oC, 29oC) days with interval testing on (1, 3, 5, 7 days). The Extraction was carried out by the maceration method with 96% ethanol as solvent. The observed parameters were concentration of chlorophyll-a, chlorophyll-b, extract brightness, and pH. The results showed that content of chlorophyll-a, chlorophyll-b, and pH had decreased with an increase in the brightness of the extract after being stored for 7 days at cold and room temperature. The higher decrease on content of chlorophyll-a by 1,32 mg/g, chlorophyll-b by 2,09 mg/g, and pH value of 6 when stored at room temperature, while in cold storage has the lowest decrease content of chlorophyll-a by 1,42 mg/g, chlorophyll- b by 2,21 mg/g and pH value by 6,2. The brightness value of the extract has the biggest increased at room temperature storage by 30,99 and the lowest is on the cold temperature by 29,25.

Immobilization of ZnO Suspension on Glass Substrate to Remove Filtration During the Removal of Remazol Red R from Aqueous Solution

2016 ◽  
Vol 12 (6) ◽  
pp. 4127-4133
Author(s):  
Nazmul Kayes ◽  
Jalil Miah ◽  
Md. Obaidullah ◽  
Akter Hossain ◽  
Mufazzal Hossain
Keyword(s):  
Aqueous Solution ◽  
Glass Substrate ◽  
Uv Light ◽  
Aqueous Suspension ◽  
Light Irradiation ◽  
Zno Films ◽  
Light Sources ◽  
Zno Film ◽  
Semiconductor Oxides ◽  
Remazol Red

Photodegradation of textile dyes in the presence of an aqueous suspension of semiconductor oxides has been of growing interest. Although this method of destruction of dyes is efficient, the main obstacle of applying this technique in the industry is the time and cost involving separation of oxides from an aqueous suspension. In this research, an attempted was made to develop ZnO films on a glass substrate by simple immobilization method for the adsorption and photodegradation of a typical dye, Remazol Red R (RRR) from aqueous solution. Adsorption and photodegradation of  RRR were performed in the presence of glass supported ZnO film. Photodegradation of the dye was carried out by varying different parameters such as the catalyst dosage, initial concentrations of RRR, and light sources. The percentage of adsorption as well as photodegradation increased with the amount of ZnO, reaches a maximum and then decreased. Maximum degradation has been found under solar light irradiation as compared to UV-light irradiation. Removal efficiency was also found to be influenced by the pre-sonication of ZnO suspension.

Etude sur la teneur en pigment de frênes qui croissent dans des conditions différentes

1973 ◽  
Vol 37 ◽  
Author(s):  
N. Lust
Keyword(s):  
Chlorophyll A ◽  
Strong Influence ◽  
The Other ◽  
Pigment Content ◽  
Chlorophyll B ◽  
Light Quantity ◽  
Yellow Pigments ◽  
The One ◽  
Green Pigments

Pigment content of ashes grown up under different circumstances - The pigment content (chlorophyll a, chlorophyll b,  xanthophyll and carotene) has been researched with ashes grown up under  different light circumstances and varying in age and height.     The results prove that the general laws concerning the influence of light  on the pigment content, don’t always work.     The phenomen is very complex. The light quantity is very important in some  cases, but insignificant in others. It seems origin and height of plants have  a strong influence. The results prove also the influence of the environment  is much higher on small plants as on big ones.     The research indicates finally the correlation between the green pigments,  the yellow pigments, and between the green pigments on the one side and the  yellow ones on the other side.

scholarly journals Shading Effects on Leaf Gas Exchange, Leaf Pigments and Secondary Metabolites of Polygonum minus Huds., an Aromatic Medicinal Herb

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 608
Author(s):  
Fairuz Fatini Mohd Yusof ◽  
Jamilah Syafawati Yaacob ◽  
Normaniza Osman ◽  
Mohd Hafiz Ibrahim ◽  
Wan Abd Al Qadr Imad Wan-Mohtar ◽  
...  
Keyword(s):  
Gas Exchange ◽  
Secondary Metabolites ◽  
Chlorophyll A ◽  
Leaf Gas Exchange ◽  
Photosynthesis Rate ◽  
Total Phenolic ◽  
Chlorophyll B ◽  
Low Light ◽  
Total Anthocyanin ◽  
Polygonum Minus

The growing demand for high value aromatic herb Polygonum minus-based products have increased in recent years, for its antioxidant, anticancer, antimicrobial, and anti-inflammatory potentials. Although few reports have indicated the chemical profiles and antioxidative effects of Polygonum minus, no study has been conducted to assess the benefits of micro-environmental manipulation (different shading levels) on the growth, leaf gas exchange and secondary metabolites in Polygonum minus. Therefore, two shading levels (50%:T2 and 70%:T3) and one absolute control (0%:T1) were studied under eight weeks and 16 weeks of exposures on Polygonum minus after two weeks. It was found that P. minus under T2 obtained the highest photosynthesis rate (14.892 µmol CO2 m−2 s−1), followed by T3 = T1. The increase in photosynthesis rate was contributed by the enhancement of the leaf pigments content (chlorophyll a and chlorophyll b). This was shown by the positive significant correlations observed between photosynthesis rate with chlorophyll a (r2 = 0.536; p ≤ 0.05) and chlorophyll b (r2 = 0.540; p ≤ 0.05). As the shading levels and time interval increased, the production of total anthocyanin content (TAC) and antioxidant properties of Ferric Reducing Antioxidant Power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) also increased. The total phenolic content (TPC) and total flavonoid content (TFC) were also significantly enhanced under T2 and T3. The current study suggested that P.minus induce the production of more leaf pigments and secondary metabolites as their special adaptation mechanism under low light condition. Although the biomass was affected under low light, the purpose of conducting the study to boost the bioactive properties in Polygonum minus has been fulfilled by 50% shading under 16 weeks’ exposure.

Mass spectrometry as a versatile ancillary technique for the rapid in situ identification of lichen metabolites directly from TLC plates

2017 ◽  
Vol 49 (5) ◽  
pp. 507-520 ◽  
Author(s):  
Pierre LE POGAM ◽  
Aline PILLOT ◽  
Françoise LOHEZIC-LE DEVEHAT ◽  
Anne-Cécile LE LAMER ◽  
Béatrice LEGOUIN ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Accurate Identification ◽  
Chemical Profiling ◽  
Analytical Strategies ◽  
Tlc Plates ◽  
Lichen Metabolites ◽  
Acetone Extracts ◽  
Layer Chromatography ◽  
Structural Classes

AbstractThin-layer chromatography (TLC) still enjoys widespread popularity among lichenologists as one of the fastest and simplest analytical strategies, today remaining the primary method of assessing the secondary product content of lichens. The pitfalls associated with this approach are well known as TLC leads to characterizing compounds by comparison with standards rather than properly identifying them, which might lead to erroneous assignments, accounting for the long-held interest in hyphenating TLC with dedicated identification tools. As such, commercially available TLC/Mass Spectrometry (MS) interfaces can be easily connected to any brand of mass spectrometer without adjustments. The spots of interest are extracted from the TLC plate to retrieve mass spectrometric signals within one minute, thereby ensuring accurate identification of the chromatographed substances. The results of this hyphenated strategy for lichens are presented here by 1) describing the TLC migration and direct MS analysis of single lichen metabolites of various structural classes, 2) highlighting it through the chemical profiling of crude acetone extracts of a set of lichens of known chemical composition, and finally 3) applying it to a lichen of unknown profile, Usnea trachycarpa.

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