scholarly journals Pertumbuhan dan Perkembangan Otak Fetus Mencit Setelah Induksi Ochratoxin A Selama Periode Organogenesis

2018 ◽  
Vol 5 (1) ◽  
pp. 15-20
Author(s):  
Arum Setiawan ◽  
Mammed Sagi ◽  
Widya Asmara ◽  
Istriyati Istriyati
Keyword(s):  
Sodium Bicarbonate ◽  
Ochratoxin A ◽  
Wall Thickness ◽  
Growth And Development ◽  
Untreated Control ◽  
Brain Weight ◽  
Brain Growth ◽  
Pregnant Mice

This experiment was performed to examine the effects of Ochratoxin A (OTA) during organogenesis period on fetuses brain growth and development. Thirty pregnant mice were divided randomly into 5 groups of 6. Ochratoxin A was dissolved in sodium bicarbonate and administered orally on seventh to fourteenth days of gestation at dosage of 0.5, 1.0, 1.5 mg/kg bw, respectively. The remaining animals were used as  an untreated control, and placebo were given by  Sodium Bicarbonate. At the age of 18-day pregnancy, mice were sacrified dan taken its brains. The fetuses brain growth and development  were observed by measure brain weight, cereberum width and length, cerebellum width and length, and the wall thichkness of cerebrum. Result of this studies indicated that OTA caused decreased of brain weight, the length and width of cerebrum and cerebellum, the wall thickness of cerebrum significantly.Key words: Ochratoxin A, brain, cerebrum, cerebellum, mice. 

scholarly journals Aluminium induced Intrauterine Growth Retardation - an experimental study

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Mamoona Nasim
Keyword(s):  
Heavy Metal ◽  
Experimental Study ◽  
Body Weight ◽  
Experimental Design ◽  
Growth And Development ◽  
Intrauterine Growth Retardation ◽  
Control Groups ◽  
Intrauterine Growth ◽  
Aluminum Sulphate ◽  
Pregnant Mice

Present study was carried out to determine the effect of aluminum containing antacid on the intrauterine growth and development of fetus. The duration of exposure was also correlated with the effects on fetal morphology and their weights. Seventy-two pregnant mice were given a daily i.e., dose of 0.7mg/100g of aluminum sulphate for various periods according to the grouping of experimental design. This dose was equivalent to maximum therapeutic dose of aluminum salt for a 70 kg man i.e. 5000mg aluminum/day. Fetal examination was performed on day 20 of gestation. The number of live and dead fetuses in the treated animals was not significantly different from the control groups. Therefore embryo lethality of aluminum cannot be induced. However there was a decrease in fetal body weight that was directly related to the duration of exposure to aluminum sulphate solution. Dissecting microscopic examination showed, the development was arrested in the groups exposed to drugs for longer periods. These results revealed that aluminum is a type of heavy metal, which is teratogenic for mammals even in doses, which are nontoxic for adults.

scholarly journals Influence of biostimulant and substrate volume on root growth and development of scarlet sage (Salvia splendens L.) transplants

2010 ◽  
Vol 55 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Svjetlana Zeljkovic ◽  
Nada Paradjikovic ◽  
Tamara Babic ◽  
Gordana Djuric ◽  
Rodoljub Oljaca ◽  
...  
Keyword(s):  
Root Growth ◽  
Growth And Development ◽  
Untreated Control ◽  
Dry Mass ◽  
Adaptation To Stress ◽  
Plant Adaptation ◽  
Salvia Splendens ◽  
Fresh And Dry Mass ◽  
Root Growth And Development

The influence of biostimulant and substrate volume on scarlet sage transplants growth and development was examined in this investigation. There was one cultivar of scarlet sage used in trial which was transplanted in pots of two different volumes. Plants were treated with biostimulant (Radifarm) in concentration of 0.25% or left untreated (control). During the trial, root and aboveground fresh and dry mass were recorded. Treatment with biostimulant and bigger substrate volume showed good results by increasing investigated parameters. Investigation shows how biostimulant application to scarlet sage transplants production improves growth and development of root and aboveground mass which is important for faster plant adaptation to stress during transplanting.

Rapid Thin Layer Chromatographic Method for Determining Aflatoxin Bi, Ochratoxin A, and Zearalenone in Corn

1978 ◽  
Vol 61 (3) ◽  
pp. 584-585
Author(s):  
Ivan Balzer ◽  
Čedo Bogdanić ◽  
Stjepan Pepeljnjak
Keyword(s):  
Sodium Bicarbonate ◽  
Thin Layer ◽  
Ochratoxin A ◽  
Ultraviolet Light ◽  
Aflatoxin B1 ◽  
Chromatographic Method ◽  
Limits Of Detection ◽  
Chloroform Extraction ◽  
Tlc Plates

Abstract A multimycotoxin thin layer chromatographic method is described for the analysis of corn. Aflatoxins are extracted from the samples with acetonitrile-water, and sodium bicarbonate is added to separate the acidic ochratoxin from zearalenone and aflatoxin B1. After chloroform extraction, 1/V NaOH is added to separate zearalenone and aflatoxin B1# The separated mycotoxins are spotted on TLC plates, which are then examined under ultraviolet light. The following recoveries ( % ) were obtained for corn samples: aflatoxin B1 71, ochratoxin A 87, and zearalenone 85. The limits of detection for the respective mycotoxins were 2, 40, and 200 ppb.

Detection of Ochratoxin A in Barley, Using Silica Gel Minicolumns

1975 ◽  
Vol 58 (1) ◽  
pp. 156-158
Author(s):  
Benedicte Hald ◽  
Palle Krogh
Keyword(s):  
Sodium Bicarbonate ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ochratoxin A ◽  
Silica Gel ◽  
Fluorescent Band ◽  
Contamination Levels ◽  
Simplified Procedure ◽  
Green Fluorescent ◽  
Layer Chromatography

Abstract A simplified procedure has been developed to detect ochratoxin A in cereals which can be used in the field where equipment for thin layer chromatography is not available. The procedure includes extraction of the acidified sample with chloroform, purification over sodium bicarbonate, and minicolumn chromatography. Under longwave ultraviolet light ochratoxin A appears as a blue-green fluorescent band at the lower end of the column. Contamination levels as low as 12 ppb can be detected by this method.

Long-chain polyunsaturated fatty acids essential for brain growth and development

Nutrition ◽  
2003 ◽  
Vol 19 (1) ◽  
pp. 66 ◽  
Author(s):  
Claudia M.aria Oller do Nascimento ◽  
Lila Missae Oyama
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Growth And Development ◽  
Brain Growth ◽  
Long Chain

scholarly journals Activity of acetic acid and sodium bicarbonate to inhibit Asergillus ochraceus on culture media and detoxification of ochratoxin A in poultry diet

2020 ◽  
Author(s):  
Halima Z. Hussein ◽  
Mneer Saed Al-Baldawy ◽  
Rakib. A. Hamed
Keyword(s):  
Acetic Acid ◽  
Sodium Bicarbonate ◽  
Ochratoxin A ◽  
Culture Media ◽  
Aspergillus Ochraceus ◽  
Toxin A ◽  
Similar Reduction

AbstractThe study was conducted to evaluate the activity of acetic acid and sodium bicarbonate to inhibit Aspergillus ochraceus on culture media and reduce ochra toxin A in poultry diet. It was found that addition of acetic acid into PDA medium 0.2%, 0.5% and 4% could reduce A ochraceus growth by 90.22%, 98.77and 100%, respectively. Similar reduction in A ochraceus growth was obtained on PDA containing sodium bicarbonate that attained to 81%, 91.5%, and 100% at 4%, 2% and 5% concentrations, respectively. Results showed that addition of acetic acid at 0.5% and sodium bicarbonate at 1% could reduce ochre A mycotoxin by 65.7% and 43.6%, respectively in the first month and then reached to 100% in the second month for the two compounds.

scholarly journals Growth and Development of Basil Transplants (Ocimum basilicum L.)under Biostimulants Application

2015 ◽  
Vol 15 (4) ◽  
pp. 415 ◽  
Author(s):  
Svjetlana Zeljković ◽  
Nada Parađiković ◽  
Uroš Šušak ◽  
Monika Tkalec
Keyword(s):  
Plant Height ◽  
Open Field ◽  
Root Development ◽  
Root Length ◽  
Growth And Development ◽  
Untreated Control ◽  
Dry Weight ◽  
Ocimum Basilicum ◽  
Plant Adaptation ◽  
Number Of Leaves

In this study, the influence of biostimulants Radifarm® on basil transplants (Ocimum basilicum L.) growth and development was examined.Seedlings transplanted into the PVC containers φ9 cm,the plants were tre­ated with the biostimulant concentration of 0.30% or untreated(control). During the experiment recorded the morphological indicators of growth and development of seedlings(plant height, number of leaves), and finally made measuring root length and fresh and dry weight of roots and above-groundparts.The aim of this study was to determine whether, to what extent and in what manner applied biostimulator impact on improved growth and root development of seedlings of basil, and thus improve the adaptation of the young seedlings in abiotical stress of transplanting into the open field. All the traits were affected by treatment with biostimulants a certain duration of the experiment.Research indicates that the use of biostimulants in the production of basil enhances the growth and development of roots and above-ground parts which is a prerequisite for faster plant adaptation on stress during transplanting.

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