scholarly journals Isolasi, Identifikasi Pigmen, dan Analisis Aktivitas Antioksidan Pigmen Monascus

2020 ◽  
Vol 12 (2) ◽  
pp. 102-108
Author(s):  
Dhanang Puspita ◽  
Isnaini K. Putri ◽  
Fitri H. Al-Janati ◽  
Milka M. Mulyanto
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Research Method ◽  
Red Pigment ◽  
Total Carotenoid ◽  
Orange Pigment ◽  
Natural Pigments ◽  
Monascus Pigments ◽  
Pharmaceutical Industries ◽  
Key Words Antioxidant

Monascus is a type of organism that can produce natural pigments. Monascus pigments have been widely used in the food and pharmaceutical industries. Besides having natural pigments, Monascus also contains antioxidants as preventing of the free radicals. The purpose of this study is to isolate, identify the type of pigment, and analyze antioxidant activity. This research method is divided into 3 stages; Monascus isolation, pigment identification with UV-vis spectrophotometer and TLC, and calculation of antioxidant activity with DPPH. The isolated Monascus had 3 pigment fractions and contained a total carotenoid of 18.55 µg/g, total orange pigment of 278.25 units/mL, and red pigment of 235.75 units/mL. Antioxidant activity of 4.22 ppm and included in the high category.  Key words: antioxidant; monascus; pigment. 

scholarly journals Characterization of functional components flour epicarp papaya (Carica papaya L) as a source of natural pigments

Respuestas ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 39-48
Author(s):  
Viviana Andrea Velasco Arango ◽  
John Edwin Sotelo Barbosa ◽  
Luis Eduardo Ordoñez Santos ◽  
José Igor Hleap Zapata
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Carica Papaya ◽  
Titratable Acidity ◽  
Carotenoid Content ◽  
Industrial Processing ◽  
Natural Pigments ◽  
Pharmaceutical Industries ◽  
Functional Components

Papaya (Carica papaya L) is some fruit rich in antioxidants and an important source for obtaining bioactive compounds. Its production, worldwide for the year 2017, was 13.3 million tons. In its industrial processing is obtained approximately between 15 and 20%, in relation to the weight of the fruit, of husks or epicarp, which are likely to be used in order to obtain organic compounds such as carotenoids and polyphenols, among others, contributing, in addition, to mitigate the effects on the environment, since generally, these shells are thrown into landfills of solid waste, generating serious problems of environmental pollution. The objective of this research was to characterize physicochemically the carotenoid pigments obtained from the papaya epicarp. A papaya epicarp flour was processed and it was determined, both in it and in the fresh epicarp, pH, titratable acidity, moisture content and dry matter. Likewise, the carotenoid content, the antioxidant activity and the content of phenolic compounds were determined. The results showed high values for physicochemical parameters. The content of carotenoid compounds for the fractions of β-carotene, α-carotene, β-cryptoxanthin, Zeaxanthin and lycopene ranged between 8,587 and 4,070 mg/100g of epicarp, with the highest value corresponding to β-cryptoxanthin and the lowest value the lycopene fraction. The antioxidant activity, expressed as inhibition of the DPPH radical, gave a value of 58.77 ± 3.038 IC50 mg/ml. The content of phenolic compounds measured in mg of gallic acid equivalents/g gave a result of 24.948 ± 0.728. The data obtained allow us to conclude that said flour can be used as a source of bioactive compounds and natural pigments both in the food industry and in the technical and pharmaceutical industries.

Characterization and Antioxidant Activities of Polysaccharides from Fructus corni

2012 ◽  
Vol 560-561 ◽  
pp. 231-236 ◽  
Author(s):  
Ping Li ◽  
Zong Gang Mou ◽  
Geng Xiu Zheng ◽  
Run Yu Ma
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Superoxide Anion ◽  
Main Chain ◽  
Antioxidant Activities ◽  
Molar Ratio ◽  
Molecular Weights ◽  
Branching Points ◽  
Pharmaceutical Industries ◽  
Hydroxyl Free Radicals

The composition and antioxidant activities of two polysaccharides PFCAⅢ and PFCCⅠ, isolated from Fructus corni, have been investigated. The monosaccharide components of PFCAⅢ were found to be rhamnose, arabinose and glucose in a molar ratio of 13.74: 50.54: 35.72, whilst PFCCⅠ was found to consist of xylose and glucose in a molar ratio of 18.8: 81.2. The weight-average molecular weights of PFCAⅢ and PFCCⅠwere 1.74×104 and 7.57×104 respectively. The polysaccharides showed potential antioxidant activity by scavenging superoxide anion (O2−∙) and hydroxyl free radicals (OH∙) and this was confirmed by their ability to inhibit oxidation of axunge and gingili. The concentrations required to scavenge 50% of hydroxyl free radicals (EC50) of PFCAⅢ and PFCCⅠ were 430μg/ml and 80μg/ml respectively, and the extents of scavenging O2−∙ by 500μg PFCAⅢ and PFCCⅠwere 15.9% and 68.0% respectively. The polysaccharide PFCCⅠ shows much more significant antioxidant activity than PFCAⅢ. PFCCⅠ was mainly linked with β(1→4)-D-glucosyl with side chains including 1-linked β-D-Glc, 1,6-linked β-D-Glc and 1,4-linked β-D-Xyl with branching points at C-6 of 1,4-linked Glc in main chain. It had the potential to be exploited by the food and pharmaceutical industries in the future with further research on this polysaccharide.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

scholarly journals Ultrasonically-Extracted Marine Polysaccharides as Potential Green Antioxidant Alternatives

Proceedings ◽  
2020 ◽  
Vol 67 (1) ◽  
pp. 23
Author(s):  
Hanaa L. Essa ◽  
Hania A. Guirguis ◽  
Mayyada M. H. El-Sayed ◽  
Dalia Rifaat ◽  
Mohamed S. Abdelfattah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Algal Species ◽  
Avicennia Marina ◽  
Ulva Lactuca ◽  
Biologically Active ◽  
Sulfated Polysaccharides ◽  
Total Phenolic ◽  
Sugar Substitutes ◽  
Pharmaceutical Industries

Marine-extracted sulfated polysaccharides (SPs) have been the subject of myriad research since they are considered an eco-friendly source of biologically active compounds. Meanwhile, food and pharmaceutical industries are urgently producing natural sugar substitutes and antioxidants as alternatives to synthetic ones which are associated with cytotoxicity and safety issues. This study assesses the potential of using marine SPs obtained via the ultrasonic-assisted extraction of different marine species, to utilize them as antioxidant sugar substitutes. The carbohydrate, total phenolic contents and antioxidant activities were measured for SP extracts of the algal species of Ulva lactuca, Jania rubens and the marine plant mangrove Avicennia marina. These SPs were structurally elucidated by Fourier Transform Infrared (FTIR) spectroscopic and high-performance liquid chromatography (HPLC) analyses. The results revealed that SPs’ highest yield percent was obtained from Ulva lactuca, 5.50 ± 0.25%. The SPs of Avicennia marina had the highest carbohydrate content, 44 ± 1% and antioxidant activity, 78.85 ± 0.06 at the 100 μg/mL concentration and 89.50 ± 0.21 at the 250 μg/mL concentration. Meanwhile, the highest phenolic content was exhibited by algal SPs obtained from Jania rubens, 132.60 ± 2.50 mgGa/g. Results also showed that all extracts have potent antioxidant activity, while the highest antioxidant activity belonged to the SPs of Avicennia marina owing possibly to their balanced glucose and galactose contents as measured by HPLC. This work emphasizes the need to consider sulfated polysaccharides from marine sources for their antioxidant activity and to correlate it with their monosaccharide content to determine the effect of reducing sugar concentration on the antioxidant activity.

PROSPECTS FOR USING ASTRAGALUS ONOBRYCHIS AS A SOURCE OF NATURAL ANTIOXIDANTS

2021 ◽  
pp. 47-53
Author(s):  
Pozdnyakovа Т.А. ◽  
Kuleshovа Е.S. ◽  
Bubenchikov R.A.
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Chemical Interaction ◽  
Raw Materials ◽  
Natural Antioxidants ◽  
The Body ◽  
Plant Raw Materials ◽  
Total Antioxidant ◽  
Promising Source ◽  
The Relationship

The predominance of synthetic and genetically modified foods in the diet of a modern person often leads to disruption of natural metabolic processes and the accumulation of toxic free radicals in the body, which provokes the development of various diseases. Natural antioxidants from medicinal plants can help maintain health and stop the processes of peroxidation. In our opinion, Аstragalus onobrychis can become a promising source of natural antioxidants. The aim of this study was to establish the antioxidant activity in extracts from the herb Astragalus onobrychis. In order to obtain objective and reliable results during our research, we used two methods: the method of titrimetry and spectrophotometry of free radicals. The titrimetric method is based on the chemical interaction between an oxidizing agent (potassium permanganate) and natural antioxidants present in extracts from plant raw materials, since they exhibit the properties of reducing agents. The spectrophotometric method makes it possible to assess the value of antioxidant activity and the duration of the reaction by assessing the interaction of 2,2-diphenyl-1-picrylhydrazil with plant antioxidants. To establish the relationship between the antioxidant activity and the presence of phenolic compounds in the plant, the content of flavonoids and hydroxycinnamic acids in the herb Astragalus onobrychis was determined. As a result of the studies, the total antioxidant activity of extracts from the herb Astragalus onobrychis was established and it was revealed that its value depends on the type of extractant used, since this factor is associated with the content of various groups of natural antioxidants in the extraction. The data obtained indicate the prospects of using the herb Astragalus onobrychis as a source of natural antioxidants.

Mechanochemical Treatment of Quercetin and Curcumin to Obtain Eutectic Mixtures with High Antioxidant Activity

CrystEngComm ◽  
2021 ◽  
Author(s):  
Richard Fernando D'Vries ◽  
Valentina Renza-Diaz ◽  
Martin emilio Gonzalez ◽  
Kriss Dayhana Pantoja
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Biological Systems ◽  
Mechanochemical Treatment ◽  
Natural Antioxidant ◽  
Eutectic Mixtures ◽  
High Antioxidant Activity ◽  
The Creation

Quercetin and curcumin are well-known natural antioxidant molecules used to prevent the malignant effect caused by free radicals on biological systems. This work addressed the creation of eutectic mixtures from...

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SPONS Stylissa sp. DENGAN MENGGUNAKAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 419
Author(s):  
Samuel I.M. Sibarani ◽  
Adithya Yudistira ◽  
Deby A. Mpila
Keyword(s):  
Antioxidant Activity ◽  
Cell Wall ◽  
Free Radicals ◽  
Ethanol Extract ◽  
Dna Bases ◽  
Oxidation Reactions ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Ethanol Extracts

ABSTRACTHabitat of sponge Stylissa sp., were under the sea and these sponges contain active compounds, which are more active than the compounds produced by terrestrial plants. Antioxidants are inhibitors of oxidation reactions due to the free radicals, which can cause weak damage to unsaturated cells, cell wall membranes, blood vessels, DNA bases, and lipid tissue, that causing disease. The study was conducted to determine the antioxidant activity of ethanol extracts  of sponge Stylissa sp., which is located on the Bangka Island, Likupang District, North Minahasa Regency. Sponge Stylissa sp., was extracted by maceration with using ethanol. Testing of antioxidant activity was carried out by the DPPH method measured by a UV-Vis spectrophotometer. The results of the study showed that ethanol extracts of sponge Stylissa sp., has antioxidant activity in each concentration and the highest at a concentration of 100 mg / L. The conclusion is a ethanol extract of sponge Stylissa sp. have high antioxidant activity with a concentration of 25 mg/L (77,40%), concentration of 50 mg/L (85,63%), concentration of 75 mg/L (88,66%), and concentration 100 mg/L (88,96%). Key words: Stylissa sp. Sponge, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl) ABSTRAKHabitat dari spons Stylissa sp. terdapat di bawah laut dan spons ini mengandung senyawa aktif yang persentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Antioksidan adalah zat penghambat reaksi oksidasi akibat radikal bebas yang dapat menyebabkan kerusakan lemah tak jenuh, membran dinding sel, pembuluh darah, basa DNA, dan jaringan lipid sehingga menimbulkan penyakit. Penelitian dilakukan untuk mengetahui aktivitas antioksidan ekstrak etanol biota laut spons Stylissa sp. yang terdapat di pulau Bangka, Kecamatan Likupang, Kabupaten Minahasa Utara. Spons Stylissa sp. ini diekstrak dengan metode maserasi menggunakan etanol. Pengujian aktivitas antioksidan ini dilakukan dengan metode DPPH yang diukur dengan alat spektrofotometer UV-Vis. Hasil dari penelitian menunjukan bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan disetiap konsentrasi dan yang tertinggi pada konsentrasi 100 mg/L. Kesimpulan yang didapat bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan yang tinggi dengan konsentrasi 25 mg/L (77,40%), konsentrasi 50 mg/L (85,63%), konsentrasi 75 mg/L (88,66%), dan konsentrasi 100 mg/L (88,96%). Kata Kunci : Spons Stylissa sp., Antioksidan, DPPH (1,1-difenil-2-pikrilhidrazil)

scholarly journals Formulation and Evaluation of Antioxidant Peel-Off Face Mask Containing Red Dragon Fruit Rind Extract (Hylocereus polyrhizus Haw.)

2020 ◽  
Vol 20 (3) ◽  
pp. 438
Author(s):  
Trasna Arman Jani ◽  
Aliefman Hakim ◽  
Yohanes Juliantoni
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Face Mask ◽  
Hexane Extract ◽  
Product Evaluations ◽  
Drying Time ◽  
Dragon Fruit ◽  
Dispersive Power

Peel-Off face mask is one of the cosmetics that is used to treat skin from free radicals. Peel-off face mask can minimize the effects of free radicals because it contains antioxidant. One of the antioxidant sources is red dragon fruit’s rind (Hylocereuspolyrhizus Haw.). The aim of this study is to obtain Peel-Off face mask of red dragon fruit’s rind extract that contains anthocyanin. Red dragon fruit’s rind was macerated by ethanol 96%, ethyl acetate and n-hexane added citric acid (4:1) solvents. TLC test used Chloroform: ethyl acetate: n-butanol (5:4:1) eluent and sprayed by AlCl3. Antioxidant activity of extract was tested using DPPH (1,1-Diphenyl-2-Picryhidrazyl) method. Peel-Off face mask was made using PVA, HPMC, methyl paraben, propylparaben, propylenglycol, extract, ethanol 96% and aquadest. The rendemen of ethanol 96% extract was 9,476%, ethyl acetate extract was 0,783% and n-hexane extract was 0,631%. The results of TLC test showed yellow spots on the ethyl acetate and ethanol 96% extracts which indicated that extract contained flavonoids (anthocyanin). The results of antioxidant activity test showed that IC50 value of ethanol 96% extract was 189,7422 (AAI=0,2087), ethyl acetate extract was 196,9398 (AAI=0,2011), and n-hexane extract was 385,3664 (AAI=0,1027). The result of the product evaluations showed that all the formulas complied the product requirements such as the organoleptic, homogenity, pH, dispersive power and drying time. Peel-off face mask already meet the product requirements but further research is needed to test the product stability and activity.

scholarly journals Berries and Leaves of Actinidia kolomikta (Rupr. & Maxim.) Maxim.: A Source of Phenolic Compounds

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 147
Author(s):  
Laima Česonienė ◽  
Paulina Štreimikytė ◽  
Mindaugas Liaudanskas ◽  
Vaidotas Žvikas ◽  
Pranas Viškelis ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Total Content ◽  
Ascorbic Acid Content ◽  
Free Radical Scavenging ◽  
Raw Material ◽  
Quantitative Composition ◽  
Pharmaceutical Industries

Berries of Actinidia kolomikta (A. kolomikta) are known for high ascorbic acid content, but the diversity of phenolic compounds has been little studied. The present research aimed to investigate phenolic compounds and antioxidant activity in berries and leaves of twelve A. kolomikta cultivars. The UHPLC-ESI-MS/MS technique was used to determine differences among cultivars in the quantitative composition of individual phenolic compounds. Antioxidant activity was determined by DPPH• free radical scavenging and CUPRAC methods. In the present study, 13 phenolic compounds were detected in berries, whereas leaves contained 17 phenolic compounds. Flavonols were the primary class found in both berries and leaves; other identified phenolic compounds were flavan-3-ols, flavones and, phenolic acids; and dihydrochalcone phloridzin was identified in the leaves. The amount and variety of phenolic compounds in berries and leaves and antioxidant activity were found to be cultivar-dependent. The highest total content of phenolic compounds was found in the leaves of the cultivar ‘Aromatnaja’ and in the berries of the cultivar ‘VIR-2’. Results of this study have confirmed that berries and leaves of A. kolomikta could be a valuable raw material for both food and pharmaceutical industries.

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

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