scholarly journals Studi Awal Regenerasi Sowang (Xanthostemon novaguineense Valet.)

2018 ◽  
Vol 9 (1) ◽  
pp. 31-36
Author(s):  
Sri Wilujeng ◽  
Verena Agustini
Keyword(s):  
Plant Regeneration ◽  
Seed Germination ◽  
Key Words ◽  
Human Activities ◽  
Land Conversion ◽  
Endemic Plant ◽  
Randomized Design ◽  
Chemical Soil ◽  
Completely Randomized Design ◽  
Set Up

Sowang (Xanthostemon novaguineense Valet.) is an endemic plant of Cycloops and strictly distributed. Since years, the population of the plants is threatened by human activities through land conversion, forest burning and logging. There are only few seedling grow in Cycloops naturally. Sowang wood is known as a good quality, because of its inherent hardness and high density. Scienfific information about this species is remain unclear. Regarding the plant regeneration, it is also not enough information so far. The study was conducted at three places around Cycloops areas namely Waena, Doyo Baru (sowing habitat) dan Wambena (no sowang areas) of Jayapura Regency, Papua and also greenhouse of FMIPA Cenderawasih University. The experimental units were set up in a completely randomized design with 3 treatments. Each experiment was carried out in three replicated and each replicate includes 100 seeds. Data were analyzed by analysis of variance (ANOVA), followed by LSD α=0.05. The result showed that there is no relation between geographical distribution of Sowang at Cycloops and the phisical and chemical soil. All type of soil used in this study was good as media of the seed germination. Regarding the ratio of the sapling population, the germination is still low only 18%, the seedling only survive for 38 days after sowing. Key words: Cycloops, regeneration, Sowang, Papua.

scholarly journals Resistance to nematode Meloidogyne paranaensis in Arabica coffee genotypes introgressed with Coffea liberica

2020 ◽  
pp. 1236-1241
Author(s):  
Gustavo H. Sera ◽  
Filipe G. Carvalho ◽  
Inês C. de B. Fonseca ◽  
Luciana H. Shigueoka ◽  
Santino A. da Silva ◽  
...  
Keyword(s):  
Germplasm Bank ◽  
Breeding Programs ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Mother Plants ◽  
Completely Randomized Design ◽  
Set Up ◽  
Moderately Resistant ◽  
Reproduction Factor ◽  
Coffea Liberica

The aim of this study was to prove that Arabica coffee introgressed with C. liberica, have resistance to Meloidogyne paranaensis (Mp). Open pollinated fruits were harvested from mother plants of 29 Arabica coffee genotypes from the IAPAR germplasm bank. Seeds were collected from the fruits and were sown to obtain seedlings to test the resistance to Mp. The experiment was set up in a completely randomized design with 29 coffee genotypes, 8 replications, and one plant per plot. Cultivars Catuaí Vermelho IAC 99 and IPR 100 were used as susceptible and resistant checks, respectively. Seedlings with three to four pairs of leaves were inoculated with 1,400 eggs and juveniles J2 of Mp (IP). At 120 days after inoculation, seedlings were evaluated by counting the nematodes per gram of roots, and the final nematodes population was obtained (FP). The reproduction factor (RF) was calculated using the formula: RF = FP/IP. The reproduction factor reduction was used to classify the resistance levels of genotypes, which were classified as highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) an highly susceptible (HS). All genotypes differed from Catuaí in resistance factor (RF), five of which did not differ from IPR 100 for RF, and only the line IAPAR 15242 had RF < 1.0. Out of 28 Arabica genotypes introgressed with C. liberica, five HR, 11 R, 11 MR and one MS were identified. However, only IAPAR 15242 and IPR 100 were classified as HR and presented 100% of HR plants, but only the first showed an RF < 1.0. Results revealed that these Arabica coffee genotypes with introgression of C. liberica genes have great potential to be used in breeding programs and they are a new alternative as a source of resistance.

scholarly journals Response of Lupinus bracteolaris seeds to pre-germinative treatments and experimental conditions

2021 ◽  
Vol 12 (2) ◽  
pp. 51-66
Author(s):  
Luciana Pinto Paim ◽  
Eduarda Demari Avrella ◽  
Juliana Carolina Alves Horlle ◽  
Claudimar Sidnei Fior ◽  
Marília Lazarotto ◽  
...  
Keyword(s):  
Seed Germination ◽  
Substrate Temperature ◽  
Water Intake ◽  
White Light ◽  
Green Light ◽  
Experimental Conditions ◽  
Germination Time ◽  
Randomized Design ◽  
Germination Speed ◽  
Completely Randomized Design

Contextualization: Lupinus bracteolaris is a heliophite species that vegetates low lands, open slopes and sandstone hills. Knowledge gap: Despite the high rusticity and potential for covering poor soils, there is still no information to assist in the analysis of its seeds. Purpose: To determine pre-germinative treatments, substrate, temperature, luminosity and water intake in the vigor of Lupinus bracteolaris seeds. Methodology: Fruits were collected from 20 matrix plants, then the seeds were processed and submitted to the following tests: pre-germinative methods (six treatments at 25°C); substrates and temperatures (sand and paper at 20, 25 and 30°C); photoblast test (white light, diffuse-green light and continuous dark under 20°C) and the imbibition curve (control and scarification between sandpaper under 20°C). Evaluations: percentage of germination and formed seedlings, germination speed index, average germination time and seedling formation, using a completely randomized design. Results and conclusions: According to the analyzed variables, the results showed superiority for the pre-germinative treatment between sandpapers for 20 seconds. In addition, the substrate paper germibox and the temperature of 20°C were the most suitable during seed germination. The seeds of Lupinus bracteolaris were classified as neutral-photoblastic and without dormancy.

scholarly journals Chilling of Endodormant Peach Propagules: I. Seed Germination and Emergence

1993 ◽  
Vol 118 (2) ◽  
pp. 248-252 ◽  
Author(s):  
James W. Frisby ◽  
Schuyler D. Seeley
Keyword(s):  
Seed Germination ◽  
Repeated Measures ◽  
Seedling Emergence ◽  
Weak Correlation ◽  
Randomized Design ◽  
Completely Randomized Design

We compared peach [Prunus persica (L.) Batsch cv. Johnson Elberta] seed germination (G) and seedling emergence (E) after various stratification (St) treatments. Treatments were arranged in factorial combinations of five St durations (20 to 60 days) at eight constant temperatures (0 to 18C) in a completely randomized design followed by repeated measures during forcing time. G and E were recorded every 5 days during forcing. Seed St at 0 to 10C and 0 to 14C promoted G and E, respectively. G and E increased with longer St treatments at promoting temperatures. There was a weak correlation between G and E averaged over the forcing measurements (r2 = 0.54). The best correlation was between E after 15 days and G after 10 days (r2 = 0.83). The results indicate that G and E in peach are not identical indicators of endodormancy (ED) release and should not be used interchangeably. Forcing times must be considered when making comparisons between G and E.

scholarly journals Development of Liquid Enhancer for Germination of Drought-stressed Oryza sativa subsp. indica Seed cv. MR284

2019 ◽  
pp. 1-9 ◽  
Author(s):  
S. N. Mahadi ◽  
F. Zawawi ◽  
R. Nulit ◽  
M. H. Ibrahim ◽  
N. I. Ab. Ghani
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Germination Percentage ◽  
Leaf Length ◽  
Seed Vigor ◽  
Rice Seed ◽  
Combination Treatments ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Selection Of

Aim: This study was conducted to develop liquid enhancer containing KCl, TU, GA, and SA for germination of drought-stressed Oryza sativa subsp. indica cv. MR284 seed. Study Design: All experiments were conducted in a completely randomized design. Two steps were involved in the development process which are to select an ideal concentration for each KCl, TU, GA, and SA, and to find an ideal combination of chemicals from the selection of ideal concentrations acquired in step 1 to form liquid enhancer. There were 20 treatments for step 1 and 9 treatments for step 2. All of these treatments with 6 replicates. Place and Duration of Study: Department of Biology, Faculty of Science, University Putra Malaysia, between June 2018 and December 2018. Methodology: The sterilized rice seed cv. MR284 was stressed in the -1.2 Mpa PEG 6000 solution for three days and germinated in the KCl, TU, GA, and SA solution in a series of concentration for 10 days, in a controlled room. Seed germination was observed daily. Results: In the first step, drought-stressed rice seed showed the best germination performance in the 30 mM of KCl, 2.0 mM of TU, 0.24 mM GA, and 0.5 mM SA. Meanwhile, in the second step, the drought-stressed rice seed showed the best germination performance in the combination of 30 mM KCl + 2.0 mM TU + 0.24 mM GA + 0.5 mM SA. The best germination performance was evaluated by the highest germination percentage (%), germination index, seed vigor, leaf length, root length and biomass. Conclusion: Therefore, the combination treatments of 30 mM KCl + 2.0 mM TU + 0.5 mM SA was found to be the most effective and simplest liquid enhancer formula that has an ability to enhance seed germination of drought-stressed rice cv. MR284 seed.

scholarly journals Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

2016 ◽  
Vol 44 (2) ◽  
pp. 133
Author(s):  
Cucu Gunarsih ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Muhamad Syukur
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Callus Induction ◽  
Rainfed Rice ◽  
Randomized Design ◽  
Doubled Haploid Plants ◽  
Completely Randomized Design ◽  
N6 Medium ◽  
Haploid Plants

ABSTRACT<br /><br />The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).<br /><br />Keywords: Callus induction, doubled haploid, rice<br /><br />

scholarly journals Comparison between Single and Double Injection of Pituitary Gland (PG) on the Breeding Performance of Climbing Perch, Anabas Testudineus (Bloch)

1970 ◽  
Vol 14 ◽  
pp. 57-60
Author(s):  
Shahid Uddin Mahmood
Keyword(s):  
Pituitary Gland ◽  
Key Words ◽  
Single Injection ◽  
Breeding Performance ◽  
Female Fish ◽  
Anabas Testudineus ◽  
Double Injection ◽  
Climbing Perch ◽  
Randomized Design ◽  
Completely Randomized Design

An investigation was carried out to study the effects of single and double injection of given dose of pituitary gland (PG) on the breeding performance viz., ovulation response, fertilization, and hatching of climbing perch, Anabas testudineus was tested. Pituitary gland dose of 1.2 mg/100g was used in this experiment. Twenty female fish was arranged into two treatments: T1 and T2, each with 10 replications. In T1, the entire dose was administered in a single injection and in T2 the same dose was given in two equal injections three hr apart. The experiment was laid in completely randomized design (CRD). It was found that administration of PG in split doses (double injection) had no additional advantages (ovulation 100%, fertilization 81.56% and hatching 72.68%) over the single injection (ovulation 100%, fertilization 83.03% and hatching 75.48%) treatment. Similarly, the ovulation time of the fish did not vary significantly in either single or double injection of PG (single injection 7.12 hr; double injection 7.20 hr). The results demonstrated that splitting of pituitary gland dose (double injection) has no additional advantages on breeding performance of A. testudineus. Therefore, single injection may be preferred over the second injection for breeding of this fish. Key words: Breeding, pituitary gland (PG), fertilization, hatching J. bio-sci. 14: 57-60, 2006

OCDs in Completely Randomized Design Set-Up

2015 ◽  
pp. 13-26
Author(s):  
Premadhis Das ◽  
Ganesh Dutta ◽  
Nripes Kumar Mandal ◽  
Bikas Kumar Sinha
Keyword(s):  
Randomized Design ◽  
Completely Randomized Design ◽  
Set Up

scholarly journals In vitro development of Encyclea cordigera in different concentrations of honey

2015 ◽  
Vol 46 (4) ◽  
pp. 590-592 ◽  
Author(s):  
Cibele Mantovani ◽  
Kathia Fernandes Lopes Pivetta
Keyword(s):  
Culture Media ◽  
Leaf Length ◽  
In Vitro Development ◽  
Randomized Design ◽  
Sucrose Medium ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Set Up ◽  
Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

scholarly journals Initial development of lettuce in vermicompost at higher trichoderma doses

2018 ◽  
Vol 36 (1) ◽  
pp. 77-82
Author(s):  
Maria MS Wiethan ◽  
Gabriel S Bortolin ◽  
Renata S Pinto ◽  
Antonio Carlos F Silva
Keyword(s):  
Seed Germination ◽  
Leaf Biomass ◽  
Chemical Characteristics ◽  
Biological Product ◽  
Initial Development ◽  
Lettuce Seeds ◽  
Randomized Design ◽  
Emergency Stabilization ◽  
Completely Randomized Design ◽  
High Concentrations

ABSTRACT The study aimed to evaluate the initial development, as well as, the chemical characteristics of lettuce plants grown in substrate with high concentrations of bioagent (trichoderma). A completely randomized design with four replicates was used. The treatments, applied to a substrate composed of a blend of vermicompost and commercial substrate, were established by the following doses of the commercial product ICB Nutrissolo Trichoderma: T1) 0.0; T2) 1.0; T3) 2.0; T4) 4.0; T5) 8.0 and T6) 16.0x1011 conidia kg-1 of product. After inoculation, the substrate was placed in trays, where 50 lettuce seeds were sown on each replication. Plants were thinned after emergency stabilization, maintaining ten seedlings per tray. At 28 days after sowing, the characteristics percentage and emergence speed index, leaf area, fresh and dry leaf biomass, volume, length and surface area of the root were evaluated. We also determined the chemical characteristics of the leaves. The percentage of seed germination and the emergence speed index were lower at doses higher than 4.0x1011 conidia kg-1 of the biological product. All the studied doses influenced negatively in shoot and root development of the plants. The percentage of nutrients in the leaves was higher in treatments with ICB.

scholarly journals Trichoderma Contributes to the Germination and Seedling Development of Açaí Palm

Agriculture ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 456
Author(s):  
Bruno Fróes Campos ◽  
Anselmo Junior Corrêa Araújo ◽  
Cristina Aledi Felsemburgh ◽  
Thiago Almeida Vieira ◽  
Denise Castro Lustosa
Keyword(s):  
Dry Mass ◽  
Initial Growth ◽  
Randomized Design ◽  
Trichoderma Isolate ◽  
Number Of Leaves ◽  
Collar Diameter ◽  
Completely Randomized Design ◽  
Set Up ◽  
Root Size ◽  
Trichoderma Isolates

The application of Trichoderma fungus can be used to obtain seedlings of higher quality for plantations. The objective of this study was to evaluate the effects of Trichoderma isolates on the germination and initial growth of açaí seedlings. Two trials were set up. In the laboratory, the seeds were inoculated in suspensions prepared with each of the isolates for 24 h, and they were arranged in trays containing filter paper in a completely randomized design with five replicates containing 50 seeds each. For the control, the seeds were submerged only in water. Part of the seedlings resulting from seeds treated and not treated with Trichoderma were transferred to bags containing forest soil and were submitted to seven modes of application of Trichoderma and kept in a nursery. The speed of germination and hypocotyl were not influenced by the biological treatment. The application of the different Trichoderma isolates positively influenced the height, collar diameter, number of leaves, root size, leaf area and root, and aerial part dry mass of the açaí seedlings. The application of the Trichoderma isolate TAM01 on the preplanting substrate and biweekly applications on the postplanting substrate was the combination that most positively influenced the development of açai seedlings.

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