Blood Glutathione Peroxidase-1 mRNA Levels Can Be Used as Molecular Biomarkers to Determine Dietary Selenium Requirements in Rats

2009 ◽  
Vol 234 (11) ◽  
pp. 1271-1279 ◽  
Author(s):  
Roger A. Sunde ◽  
Kevin M. Thompson ◽  
Jacqueline K. Evenson ◽  
Britta M. Thompson
Keyword(s):  
Glutathione Peroxidase ◽  
Molecular Biomarkers ◽  
Mrna Levels ◽  
Dietary Selenium ◽  
Glutathione Peroxidase 1

scholarly journals Longitudinal selenium status in healthy British adults: assessment using biochemical and molecular biomarkers

2008 ◽  
Vol 99 (S3) ◽  
pp. S37-S47 ◽  
Author(s):  
Roger A. Sunde ◽  
Elaine Paterson ◽  
Jacqueline K. Evenson ◽  
Kimberly M. Barnes ◽  
Julie A. Lovegrove ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Glutathione Peroxidase ◽  
Biochemical Markers ◽  
Molecular Biomarkers ◽  
Mrna Levels ◽  
Phospholipid Hydroperoxide Glutathione Peroxidase ◽  
Response Curves ◽  
Glutathione Peroxidase 1 ◽  
Longitudinal Effects ◽  
Se Status

Human selenium (Se) requirements are currently based on biochemical markers of Se status. In rats, tissue glutathione peroxidase-1 (Gpx1) mRNA levels can be used effectively to determine Se requirements; blood Gpx1 mRNA levels decrease in Se-deficient rats, so molecular biology-based markers have potential for human nutrition assessment. To study the efficacy of molecular biology markers for assessing Se status in humans, we conducted a longitudinal study on 39 subjects (age 45 ± 11) in Reading, UK. Diet diaries (5 day) and blood were obtained from each subject at 2, 8, 17 and 23 weeks, and plasma Se, glutathione peroxidase (Gpx3) enzyme activity, and selenoprotein mRNA levels were determined. There were no significant longitudinal effects on Se biomarkers. Se intake averaged 48 ± 14 μg/d. Plasma Se concentrations averaged 1·13 ± 0·16 μmol/l. Plasma Se v. energy-corrected Se intake (ng Se/kJ/d) was significantly correlated, but neither Gpx3 activity v. Se intake (ng Se/kJ/d) nor Gpx3 activity v. plasma Se was significantly correlated. Collectively, this indicates that subjects were on the plateaus of the response curves. Selenoprotein mRNAs were quantitated in total RNA isolated from whole blood, but mRNA levels for Gpx1, selenoprotein H, and selenoprotein W (all highly regulated by Se in rodents), as well selenoprotein P, Gpx3, and phospholipid hydroperoxide glutathione peroxidase were also not significantly correlated with plasma Se. Thus selenoprotein molecular biomarkers, as well as traditional biochemical markers, are unable to further distinguish differences in Se status in these Se replete subjects. The efficacy of molecular biomarkers to detect Se deficiency needs to be tested in Se-deficient populations.

scholarly journals Assessment of Glutathione Peroxidase-1 (GPX1) Gene Expression as a Specific Diagnostic and Prognostic Biomarker in Malignant Pleural Mesothelioma

Diagnostics ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2285
Author(s):  
Amal F. Gharib ◽  
Muhammad Alaa Eldeen ◽  
Amany Salah Khalifa ◽  
Wael H. Elsawy ◽  
Emad Mohamed Eed ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glutathione Peroxidase ◽  
Malignant Pleural Mesothelioma ◽  
Success Factors ◽  
Malignant Tumors ◽  
University Hospital ◽  
Pleural Mesothelioma ◽  
Mrna Levels ◽  
Expression Levels ◽  
Glutathione Peroxidase 1

Malignant pleural mesothelioma (MPM) is a malignant tumor of the mesothelial lining of the thorax. It has been related to frequent exposure to asbestos. Diagnosis of malignant pleural mesothelioma is considered a criticizing problem for clinicians. Early diagnosis and sufficient surgical excision of MPM are considered the cornerstone success factors for the management of early MPM. Glutathione peroxidase-1 (GPX1) is an intracellular protein found to be extensively distributed in all cells, and it belongs to the GPX group. In the current study, we included ninety-eight patients with MPM that underwent surgery at the Zagazig University Hospital in Egypt. We assessed GPX1 gene expression level as it was thought to be related to pathogenicity of cancer in a variety of malignant tumors. We observed a significant elevation in GPX1-mRNA levels in MPM relative to the nearby normal pleural tissues. It was found to be of important diagnostic specificity in the differentiation of MPM from normal tissues. Moreover, we studied the survival of patients in correlation to the GPX1 expression levels and we reported that median overall survival was about 16 months in patients with high GPX1 expression levels, while it was found to be about 40 months in low GPX1 levels.

Selenium regulation of transcript abundance and translational efficiency of glutathione peroxidase-1 and −4 in rat liver

2001 ◽  
Vol 357 (3) ◽  
pp. 851-858 ◽  
Author(s):  
Sherri WEISS SACHDEV ◽  
Roger A. SUNDE
Keyword(s):  
Glutathione Peroxidase ◽  
Rat Liver ◽  
Transcript Abundance ◽  
Translational Efficiency ◽  
Mrna Levels ◽  
Glutathione Peroxidase 1 ◽  
Relative Contribution ◽  
Se Deficiency ◽  
Selenoprotein Mrnas ◽  
Se Status

Glutathione peroxidase (GPX)1 mRNA in rat liver falls dramatically during Se deficiency to levels that are approx. 10% of Se-adequate levels. This regulation is mediated by mRNA stability, and is hypothesized to involve nonsense-mediated mRNA decay. mRNA levels for GPX4 and other selenoproteins are much less regulated by Se status. To evaluate the relative contribution of mRNA abundance versus translational efficiency to overall regulation of GPX1 expression, we quantified GPX1, GPX4 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts per cell in rat liver. Surprisingly, we found that GPX1 transcripts in Se deficiency are moderately abundant and similar in abundance to GAPDH and other selenoprotein mRNAs; Se supplementation increases GPX1 mRNA so that it is 30-fold higher than GAPDH mRNA. Translational efficiency of GPX1 mRNA is half of that of GPX4. Translational efficiency of GPX1 mRNA increases approx. 20-fold with Se supplementation and appears to switch GPX1 mRNA from nonsense-mediated degradation to translation. This regulatory switch can explain why GPX1 expression is an excellent parameter for assessment of Se status.

scholarly journals Dietary Selenium Deficiency Partially Rescues Type 2 Diabetes–Like Phenotypes of Glutathione Peroxidase-1–Overexpressing Male Mice

2012 ◽  
Vol 142 (11) ◽  
pp. 1975-1982 ◽  
Author(s):  
Xi Yan ◽  
Matthew P. Pepper ◽  
Marko Z. Vatamaniuk ◽  
Carol A. Roneker ◽  
Li Li ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Glutathione Peroxidase ◽  
Selenium Deficiency ◽  
Male Mice ◽  
Dietary Selenium ◽  
Glutathione Peroxidase 1
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