Gestational and Postnatal Low Protein Diet Alters Insulin Sensitivity in Female Rats

2009 ◽  
Vol 234 (12) ◽  
pp. 1437-1444 ◽  
Author(s):  
Kally J. Berleze ◽  
Alexandre P. Müller ◽  
Ingrid D. Schweigert ◽  
Aline Longoni ◽  
Fernanda Sordi ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
Protein Diet ◽  
Female Rats ◽  
Low Protein Diet ◽  
Low Protein

scholarly journals Vagotomy Reduces Insulin Clearance in Obese Mice Programmed by Low-Protein Diet in the Adolescence

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Camila Lubaczeuski ◽  
Luciana Mateus Gonçalves ◽  
Jean Franciesco Vettorazzi ◽  
Mirian Ayumi Kurauti ◽  
Junia Carolina Santos-Silva ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Insulin Sensitivity ◽  
Glucose Tolerance ◽  
High Fat Diet ◽  
Insulin Clearance ◽  
Protein Diet ◽  
Low Protein Diet ◽  
High Fat ◽  
Insulin Degrading Enzyme ◽  
Low Protein

The aim of this study was to investigate the effect of subdiaphragmatic vagotomy on insulin sensitivity, secretion, and degradation in metabolic programmed mice, induced by a low-protein diet early in life, followed by exposure to a high-fat diet in adulthood. Weaned 30-day-old C57Bl/6 mice were submitted to a low-protein diet (6% protein). After 4 weeks, the mice were distributed into three groups: LP group, which continued receiving a low-protein diet; LP + HF group, which started to receive a high-fat diet; and LP + HFvag group, which underwent vagotomy and also was kept at a high-fat diet. Glucose-stimulated insulin secretion (GSIS) in isolated islets, ipGTT, ipITT, in vivo insulin clearance, and liver expression of the insulin-degrading enzyme (IDE) was accessed. Vagotomy improved glucose tolerance and reduced insulin secretion but did not alter adiposity and insulin sensitivity in the LP + HFvag, compared with the LP + HF group. Improvement in glucose tolerance was accompanied by increased insulinemia, probably due to a diminished insulin clearance, as judged by the lower C-peptide : insulin ratio, during the ipGTT. Finally, vagotomy also reduced liver IDE expression in this group. In conclusion, when submitted to vagotomy, the metabolic programmed mice showed improved glucose tolerance, associated with an increase of plasma insulin concentration as a result of insulin clearance reduction, a phenomenon probably due to diminished liver IDE expression.

Low-protein diet impairs vascular relaxation in virgin and pregnant rats

2002 ◽  
Vol 102 (5) ◽  
pp. 553-560 ◽  
Author(s):  
Angeliki KOUMENTAKI ◽  
Frederick ANTHONY ◽  
Lucilla POSTON ◽  
Timothy WHEELER
Keyword(s):  
Cardiac Output ◽  
Plasma Volume ◽  
Vascular Function ◽  
Mesenteric Arteries ◽  
Protein Diet ◽  
Female Rats ◽  
Low Protein Diet ◽  
Pregnant Rats ◽  
No Donor ◽  
Low Protein

Pregnancy is associated with increases in maternal cardiac output and plasma volume and a reduction in peripheral vascular resistance. Cardiac output and plasma volume are substantially reduced in pregnant rats fed a low-protein diet, but it is not known whether vascular function is also compromised. We have investigated vascular function in virgin and pregnant Wistar rats subjected to dietary protein restriction [9% (w/v) casein, compared with 18% (w/v) casein for controls]. The diets were fed to the groups for 18 days; in the pregnant rats, the diets were given from day 1 of pregnancy. Branches of the mesenteric arteries were studied on day 18 of the dietary period using myography. Significant reductions in sensitivity to acetylcholine occurred in vessels from virgin (P = 0.04) and pregnant (P = 0.01) rats that had consumed the 9% casein diet. In arteries from the virgin rats on the restricted diet there was also a significant reduction in sensitivity (P = 0.0003) and maximum relaxation (P = 0.009) to the NO donor spermine NONOate. Mean placental and fetal weights were significantly lower in the rats fed on 9% casein (P<0.0001 and P = 0.005 respectively). Thus low-protein diets impair vasodilator responses in female rats. These effects may contribute to the poor cardiovascular adaptation to pregnancy and lower fetal weights associated with restricted protein intake.

High fat-low protein diet induces metabolic alterations and cognitive dysfunction in female rats

2019 ◽  
Vol 34 (6) ◽  
pp. 1531-1546 ◽  
Author(s):  
Ravinder Naik Dharavath ◽  
Shiyana Arora ◽  
Mahendra Bishnoi ◽  
Kanthi Kiran Kondepudi ◽  
Kanwaljit Chopra
Keyword(s):  
Cognitive Dysfunction ◽  
Protein Diet ◽  
Female Rats ◽  
Low Protein Diet ◽  
High Fat ◽  
Metabolic Alterations ◽  
Low Protein

THE EFFECT OF PARATHION ON THE LIVER AND KIDNEY CARBOXYLESTERASES AND THE PLASMA ACETYLCHOLINESTERASES OF RATS FED NUTRITIONALLY DEFICIENT DIETS

1966 ◽  
Vol 44 (6) ◽  
pp. 809-817 ◽  
Author(s):  
Sheila I. Read ◽  
E. J. Middleton ◽  
W. P. Mckinley
Keyword(s):  
Control Diet ◽  
Acetylcholinesterase Activity ◽  
Protein Diet ◽  
Female Rats ◽  
Male Rats ◽  
Low Protein Diet ◽  
Male And Female ◽  
Low Protein ◽  
Male And Female Rats ◽  
Liver And Kidney

Female rats were fed diets low in minerals, vitamins, or protein, or a control diet, both alone and supplemented with 10 parts per million (p.p.m.) parathion for 3 weeks. Male and female rats were fed control and tow-vitamin diets both with and without parathion supplementation (0–10 p.p.m.) for 3 weeks. The liver and kidney carboxylesterases (EC 3.1.1.1.), and the plasma acetylcholinesterases (EC 3.1.1.7.) of the male rats, were measured.In the female rats, a low-mineral diet resulted in an increase of carboxylesterases in the liver and kidney; a low-vitamin diet caused a marked increase in liver carboxylesterases but had no effect on the carboxylesterases of the kidney. Parathion at 10 p.p.m. in all diets greatly reduced the liver carboxylesterases but had less effect on kidney carboxylesterases, except in the case of the low-protein diet, for which the reduction was similar to that in the liver. Varying amounts of parathion added to the low-vitamin diet reduced the liver and kidney carboxylesterases, but to a less extent than when added to the control diet.The liver carboxylesterases of male rats were inhibited approximately 50% by 2 p.p.m. parathion in the control diet and by 4 p.p.m. parathion in the low-vitamin diet. However, inhibition of plasma acetylcholinesterase and kidney carboxylesterases was not marked until the 10 p.p.m. parathion level was fed. The acetylcholinesterase activity of the plasma of male rats did not decrease until the level of liver carboxylesterases was very low.

scholarly journals A Low Protein Diet Alters Bone Material Level Properties and the Response toIn VitroRepeated Mechanical Loading

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Victor Dubois-Ferrière ◽  
René Rizzoli ◽  
Patrick Ammann
Keyword(s):  
Cyclic Loading ◽  
Mechanical Loading ◽  
Protein Diet ◽  
Female Rats ◽  
Low Protein Diet ◽  
Nanoindentation Test ◽  
Displacement Curve ◽  
Bone Material ◽  
Low Protein

Low protein intake is associated with an alteration of bone microstructure and material level properties. However, it remains unknown whether these alterations of bone tissue could influence the response to repeated mechanical loading. The authors investigated thein vitroeffect of repeated loading on bone strength in humeri collected from 20 6-month-old female rats pair-fed with a control (15% casein) or an isocaloric low protein (2.5% casein) diet for 10 weeks. Bone specimens were cyclically loaded in three-point bending under load control for 2000 cycles. Humeri were then monotonically loaded to failure. The load-displacement curve of thein vitrocyclically loaded humerus was compared to the contralateral noncyclically loaded humerus and the influence of both protein diets. Material level properties were also evaluated through a nanoindentation test. Cyclic loading decreased postyield load and plastic deflection in rats fed a low protein diet, but not in those on a regular diet. Bone material level properties were altered in rats fed a low protein diet. This suggests that bone biomechanical alterations consequent to cyclic loading are more likely to occur in rats fed a low protein diet than in control animals subjected to the samein vitrocyclic loading regimen.

Decreased serum insulin-like growth factor I response to growth hormone in hypophysectomized rats fed a low protein diet: evidence for a postreceptor defect

1988 ◽  
Vol 117 (3) ◽  
pp. 320-326 ◽  
Author(s):  
M. Maes ◽  
Y. Amand ◽  
L. E. Underwood ◽  
D. Maiter ◽  
J.-M. Ketelslegers
Keyword(s):  
Binding Sites ◽  
Protein Diet ◽  
Female Rats ◽  
Low Protein Diet ◽  
Affinity Constant ◽  
Protein Calorie Malnutrition ◽  
Low Protein ◽  
Igf I ◽  
Hypophysectomized Rats ◽  
Gh Resistance

Abstract. In protein-calorie malnutrition, serum IGF-I concentrations are low despite high GH. This GH resistance might be due to a reduced number of liver GH binding sites as suggested by studies performed in fasted rats that were refed a low protein diet. To determine whether a postreceptor defect in GH action might also contribute to the GH resistance, we measured the number and the affinity constant of the liver GH binding sites and the serum IGF-I responses to injections of recombinant bGH in hypophysectomized female rats, fed a standard (15% protein) diet (N = 25) or a low (5%) protein diet (N = 25) for 8 days. There were no significant differences in the liver GH binding capacities between the 15% and the 5% protein-fed rats, whether expressed as pmol per liver (20.6 ± 3.5 vs 14.4 ± 1.3; mean ± sem; P < 0.2; N = 5, respectively), pmol per mg DNA (1.08 ± 0.16 vs 0.84 ± 0.07; P <0.4) or fmol per mg of protein (28.98 ± 5.04 vs 30.26 ± 2.00; P > 0.5). Likewise, the affinity constants of the GH binding sites of the 15% and the 5% protein-fed rats were not significantly different (0.78 ± 0.05 vs 0.78 ± 0.07 × 109 l/mol; P > 0.5). Despite these non-significant reductions in liver GH binding sites, the IGF-I responses 24 h after sc injections of increasing doses of bovine GH were blunted in the rats fed the 5% protein diet. The maximal IGF-I response in the rats with the normal protein intake was 360 ± 30 U/I, but only 130 ± 40 U/l in the 5% protein-fed animals (P < 0.001). The blunted serum IGF-I responses to GH, together with decreased maximal stimulation in the 5% protein-fed hypophysectomized rats, support the possibility that a postreceptor defect in GH action contributes to the GH resistance in protein-calorie malnutrition.

Low protein diet during gestation and lactation increases food reward seeking but does not modify sucrose taste reactivity in adult female rats

2016 ◽  
Vol 49 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Amanda Alves Marcelino Silva ◽  
Mayara Matias Oliveira ◽  
Taisy Cinthia Ferro Cavalcante ◽  
Larissa Cavalcanti Amaral Almeida ◽  
Julliet Araújo Souza ◽  
...  
Keyword(s):  
Adult Female ◽  
Food Reward ◽  
Protein Diet ◽  
Female Rats ◽  
Low Protein Diet ◽  
Taste Reactivity ◽  
Reward Seeking ◽  
Low Protein

The influence of simultaneous whole body exposure to cigarette smoke and low-protein diet feeding on fertility, reproduction, and progeny of rats

2002 ◽  
Vol 21 (11) ◽  
pp. 615-621 ◽  
Author(s):  
E Florek ◽  
K Szymanowski ◽  
J Wrzosek ◽  
W Piekoszewski ◽  
W Biczysko ◽  
...  
Keyword(s):  
Tobacco Smoke ◽  
Negative Influence ◽  
Smoke Exposure ◽  
Protein Diet ◽  
Female Rats ◽  
Tobacco Smoke Exposure ◽  
Low Protein Diet ◽  
Whole Body ◽  
Low Protein ◽  
Parent Generation

The aim of the present study was to evaluate the toxicological influence of different concentrations of tobacco smoke and low-protein diet on fertility, reproduction, and survival of offspring of Wistar female rats. We studied three generations, two litters in each. There were 192 animals in parent generation. Animals were exposed to three different concentrations of tobacco smoke that were reflected by the carbon monoxide (CO) concentration (500, 1000, and 1500 mg CO/m3 air) during 11 weeks (six before and two weeks during mating, and three weeks of pregnancy). Additionally, animals were divided into two subgroups that were fed normal or low-protein diet. In the present study, we concentrated on the indices describing the reproduction ability, fertility, and rearing of pups of mothers exposed to tobacco smoke. The parameters of newborns survival were estimated also. We concluded that neither tobacco smoke exposure nor the low-protein diet changed the duration of pregnancy. The tobacco smoke in all doses and low-protein diet had a negative influence on fertility, reproduction, and survival of newborns from exposed mothers. This influence was mainly attributed to the tobacco smoke exposure. The levels of carboxyhaemoglobin were increasing with increasing tobacco smoke concentration.

Sign in / Sign up

Export Citation Format

Share Document