An improved biomechanical testing protocol for evaluating spinal arthroplasty and motion preservation devices in a multilevel human cadaveric cervical model

Denis J. DiAngelo; Kevin T. Foley

doi:10.3171/foc.2004.17.3.4

An improved biomechanical testing protocol for evaluating spinal arthroplasty and motion preservation devices in a multilevel human cadaveric cervical model

Neurosurgical FOCUS ◽

10.3171/foc.2004.17.3.4 ◽

2004 ◽

Vol 17 (3) ◽

pp. 1-29 ◽

Cited By ~ 16

Author(s):

Denis J. DiAngelo ◽

Kevin T. Foley

Keyword(s):

Cervical Spine ◽

Biomechanical Testing ◽

Bending Moment ◽

Testing Methods ◽

Motion Response ◽

Testing Protocol ◽

Spinal Arthroplasty ◽

Pure Moment ◽

The Individual

Object An experimental study was performed to determine the biomechanical end-mounting configurations that replicate in vivo physiological motion of the cervical spine in a multiple-level human cadaveric model. The vertebral motion response for the modified testing protocol was compared to in vivo motion data and traditional pure-moment testing methods. Methods Biomechanical tests were performed on fresh human cadaveric cervical spines (C2–T1) mounted in a programmable testing apparatus. Three different end-mounting conditions were studied: pinned–pinned, pinned–fixed, and translational/pinned–fixed. The motion response of the individual segmental vertebral rotations was statistically compared using one-way analysis of variance and Student-Newman-Keuls tests (p < 0.05 unless otherwise stated) to determine differences in the motion responses for different testing methods. Conclusions A translational/pinned–fixed mounting configuration induced a bending-moment distribution across the cervical spine, resulting in a motion response that closely matched the in vivo case. In contrast, application of pure-moment loading did not reproduce the physiological response and is less suitable for studying disc arthroplasty and nonfusion devices.

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Use of Spine Robot Employing Real Time Force Control to Simulate a Pure Moment Protocol for the Subaxial Cervical Spine: An In Vitro Biomechancial Study

ASME 2011 Summer Bioengineering Conference, Parts A and B ◽

10.1115/sbc2011-53902 ◽

2011 ◽

Author(s):

Daniel M. Wido ◽

Denis J. DiAngelo ◽

Brian P. Kelly

Keyword(s):

Cervical Spine ◽

Real Time ◽

Force Control ◽

Biomechanical Testing ◽

Subaxial Cervical Spine ◽

Experimental Artifact ◽

Pure Moment ◽

Robotic Testing ◽

Spine Robot

A standard biomechanical testing protocol for evaluation of the sub-axial cervical spine is the application of pure bending moments to the free end of the spine (with opposing end fixed) and measurement of its motion response. The pure moment protocol is often used to compare spinal fusion instrumentation and has also been used to evaluate non-fusion instrumentation (e.g. disc arthroplasty devices) [1,2]. A variety of different testing systems have been employed to implement pure moment application. In cases where the loading is applied quasi-statically using a series of weights and pulleys the spine may relax between intermittent loading phases and/or unintended loading may be applied causing experimental artifact. Our objective was to use an existing programmable robotic testing platform (Spine Robot) to develop a novel real time force control strategy to simulate pure moment loading under precisely controlled continuous movement conditions. This would serve to advance robotic testing capabilities with an end goal to simulate different protocols in the same platform, and to potentially minimize fixturing and quasi-static artifacts.

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Backsplinting for Treatment of Thoracic and Lumbar Fracture/Luxation in the Dog: Principles of Application and Case Series

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.1055/s-0038-1633107 ◽

1992 ◽

Vol 05 (04) ◽

pp. 179-187 ◽

Cited By ~ 7

Author(s):

Gail Smith ◽

R. H. Patterson

Keyword(s):

Closed Reduction ◽

Biomechanical Testing ◽

Case Series ◽

Bending Moment ◽

Bending Test ◽

Bending Moments ◽

Lumbar Fracture ◽

Bracing System ◽

Sufficient Strength

SummarySixteen animals with thoracic and lumbar fracture/luxations were treated with an external bracing system. The splint design and the method of application are described along with the complications seen. All of the animals recovered acceptable function, although six had residual neurologic deficits. Biomechanical testing of the splint revealed sufficient strength to limit angular deformation at a bending moment of 40 Nm. This approximates calculated in vivo bending moments for a large paralysed dog. Principles for closed reduction and external bracing of thoracic and lumbar injuries are presented.External coaptation was used in the reduction and fixation of 16 animals with thoracic and lumbar fracture/luxations. This spinal bracing system is described and principles of its application are presented. An experimental bending test was employed to test the hypothesis that the splint could effectively limit angulation at calculated in vivo bending moments.

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Adaptive Hybrid Control Algorithm With Iterative Learning for Robotic In Vitro Biomechanical Testing of Spine

ASME 2012 Summer Bioengineering Conference, Parts A and B ◽

10.1115/sbc2012-80333 ◽

2012 ◽

Cited By ~ 1

Author(s):

T. F. Bonner ◽

L. Gilbertson ◽

R. W. Colbrunn

Keyword(s):

Control Algorithm ◽

Degrees Of Freedom ◽

Hybrid Control ◽

Dynamic Testing ◽

Biomechanical Testing ◽

Testing Methods ◽

Robotic Technology ◽

Control Schemes ◽

Pure Moment

In spine testing, methods have been developed to apply pure moments to a single axis of the spine to elucidate the mechanical properties of the spine. The application of those concepts continues to be applied with custom loading frames, custom robotics systems, and adaptation of commercial robotic technology. With these systems and pure moment testing, spinal biomechanics variables such as the neutral zone and range of motion can be determined. As more complex testing systems with higher degrees of freedom (DOF) capabilities are developed, dynamic testing becomes a possibility. However, these more complex testing systems require more complex control schemes.

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Preclinical Testing of Radiopharmaceuticals for the Detection and Characterization of Osteomyelitis: Experiences from a Porcine Model

Molecules ◽

10.3390/molecules26144221 ◽

2021 ◽

Vol 26 (14) ◽

pp. 4221

Author(s):

Aage Kristian Olsen Alstrup ◽

Svend Borup Jensen ◽

Ole Lerberg Nielsen ◽

Lars Jødal ◽

Pia Afzelius

Keyword(s):

Animal Model ◽

Animal Models ◽

Porcine Model ◽

Animal Experiments ◽

Radioactive Tracers ◽

The Individual ◽

Selection Of

The development of new and better radioactive tracers capable of detecting and characterizing osteomyelitis is an ongoing process, mainly because available tracers lack selectivity towards osteomyelitis. An integrated part of developing new tracers is the performance of in vivo tests using appropriate animal models. The available animal models for osteomyelitis are also far from ideal. Therefore, developing improved animal osteomyelitis models is as important as developing new radioactive tracers. We recently published a review on radioactive tracers. In this review, we only present and discuss osteomyelitis models. Three ethical aspects (3R) are essential when exposing experimental animals to infections. Thus, we should perform experiments in vitro rather than in vivo (Replacement), use as few animals as possible (Reduction), and impose as little pain on the animal as possible (Refinement). The gain for humans should by far exceed the disadvantages for the individual experimental animal. To this end, the translational value of animal experiments is crucial. We therefore need a robust and well-characterized animal model to evaluate new osteomyelitis tracers to be sure that unpredicted variation in the animal model does not lead to a misinterpretation of the tracer behavior. In this review, we focus on how the development of radioactive tracers relies heavily on the selection of a reliable animal model, and we base the discussions on our own experience with a porcine model.

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Animal Models in the Evaluation of the Effectiveness of Phage Therapy for Infections Caused by Gram-Negative Bacteria from the ESKAPE Group and the Reliability of Its Use in Humans

Microorganisms ◽

10.3390/microorganisms9020206 ◽

2021 ◽

Vol 9 (2) ◽

pp. 206

Author(s):

Martyna Cieślik ◽

Natalia Bagińska ◽

Andrzej Górski ◽

Ewa Jończyk-Matysiak

Keyword(s):

Animal Models ◽

Phage Therapy ◽

Animal Experiments ◽

Species Group ◽

Effectiveness Evaluation ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Enterobacter Species ◽

The Individual

The authors emphasize how extremely important it is to highlight the role played by animal models in an attempt to determine possible phage interactions with the organism into which it was introduced as well as to determine the safety and effectiveness of phage therapy in vivo taking into account the individual conditions of a given organism and its physiology. Animal models in which phages are used make it possible, among other things, to evaluate the effective therapeutic dose and to choose the possible route of phage administration depending on the type of infection developed. These results cannot be applied in detail to the human body, but the knowledge gained from animal experiments is invaluable and very helpful. We would like to highlight how useful animal models may be for the possible effectiveness evaluation of phage therapy in the case of infections caused by gram-negative bacteria from the ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species) group of pathogens. In this review, we focus specifically on the data from the last few years.

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Graphene and Reproduction: A Love-Hate Relationship

Nanomaterials ◽

10.3390/nano11020547 ◽

2021 ◽

Vol 11 (2) ◽

pp. 547

Author(s):

Marina Ramal-Sanchez ◽

Antonella Fontana ◽

Luca Valbonetti ◽

Alessandra Ordinelli ◽

Nicola Bernabò ◽

...

Keyword(s):

Graphene Oxide ◽

Reproductive Function ◽

Scientometric Analysis ◽

Potential Toxicity ◽

Good Tool ◽

Vitro Fertilization ◽

Number Of Publications ◽

The Individual

Since its discovery, graphene and its multiple derivatives have been extensively used in many fields and with different applications, even in biomedicine. Numerous efforts have been made to elucidate the potential toxicity derived from their use, giving rise to an adequate number of publications with varied results. On this basis, the study of the reproductive function constitutes a good tool to evaluate not only the toxic effects derived from the use of these materials directly on the individual, but also the potential toxicity passed on to the offspring. By providing a detailed scientometric analysis, the present review provides an updated overview gathering all the research studies focused on the use of graphene and graphene-based materials in the reproductive field, highlighting the consequences and effects reported to date from experiments performed in vivo and in vitro and in different animal species (from Archea to mammals). Special attention is given to the oxidized form of graphene, graphene oxide, which has been recently investigated for its ability to increase the in vitro fertilization outcomes. Thus, the potential use of graphene oxide against infertility is hypothesized here, probably by engineering the spermatozoa and thus manipulating them in a safer and more efficient way.

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ANTI-INFLAMMATORY ACTIVITY OF CURCUMIN AND CAPSAICIN AUGMENTED IN COMBINATION

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i6.18635 ◽

2017 ◽

Vol 9 (6) ◽

pp. 145

Author(s):

Thriveni Vasanth Kumar ◽

Manjunatha H. ◽

Rajesh Kp

Keyword(s):

Acetic Acid ◽

Protective Effect ◽

Vascular Permeability ◽

Diclofenac Sodium ◽

Significant Inhibition ◽

Inflammatory Activity ◽

Anti Inflammatory ◽

The Individual

Objective: Dietary curcumin and capsaicin are well known for their health beneficial potencies. The current study was done to assess the anti-inflammatory activity of curcumin, capsaicin and their combination by employing in vitro and in vivo models.Methods: We investigated the protective effect of curcumin, capsaicin and their combination using in vitro heat induced human red blood cell (HRBC) membrane stabilisation, in vivo 3% agar induced leukocyte mobilisation and acetic acid induced vascular permeability assay.Results: Curcumin, capsaicin and their combination exhibited concentration dependent protective effect against heat-induced HRBC membrane destabilisation, while combined curcumin and capsaicin restored 87.0±0.64 % membrane stability and it is found to be better than curcumin, capsaicin and diclofenac sodium (75.0±0.25. 72±0.9 and 80.0±0.31 %) protective effect. In agar suspension induced leukocyte mobilization assay, the combined curcumin and capsaicin had shown 39.5±1.58 % of inhibition compared to individual curcumin and capsaicin, which showed moderate inhibition of 16.0±3.14 and 21.6±2.17 % respectively. Besides, the combined curcumin and capsaicin had shown highly significant inhibition of acetic acid-induced vascular permeability in rats (62.0±3.14 %), whereas individual curcumin and capsaicin showed moderate inhibition of vascular permeability with 36.0±2.41 and 43.0±1.92 % respectively.Conclusion: This study demonstrates the significant anti-inflammatory property of combined curcumin and capsaicin at half of the individual concentration of curcumin and capsaicin.

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In Vivo Bioavailability of Selenium in Selenium-Enriched Streptococcus thermophilus and Enterococcus faecium in CD IGS Rats

Antioxidants ◽

10.3390/antiox10030463 ◽

2021 ◽

Vol 10 (3) ◽

pp. 463

Author(s):

Gabriela Krausova ◽

Antonin Kana ◽

Marek Vecka ◽

Ivana Hyrslova ◽

Barbora Stankova ◽

...

Keyword(s):

Glutathione Reductase ◽

Enterococcus Faecium ◽

Reductase Activity ◽

Streptococcus Thermophilus ◽

Heart Tissue ◽

Sprague Dawley ◽

Glutathione Reductase Activity ◽

Novel Concept ◽

The Individual

The selenium (Se) enrichment of yeasts and lactic acid bacteria (LAB) has recently emerged as a novel concept; the individual health effects of these beneficial microorganisms are combined by supplying the essential micronutrient Se in a more bioavailable and less toxic form. This study investigated the bioavailability of Se in the strains Enterococcus faecium CCDM 922A (EF) and Streptococcus thermophilus CCDM 144 (ST) and their respective Se-enriched forms, SeEF and SeST, in a CD (SD-Sprague Dawley) IGS rat model. Se-enriched LAB administration resulted in higher Se concentrations in the liver and kidneys of rats, where selenocystine was the prevalent Se species. The administration of both Se-enriched strains improved the antioxidant status of the animals. The effect of the diet was more pronounced in the heart tissue, where a lower glutathione reductase content was observed, irrespective of the Se fortification in LAB. Interestingly, rats fed diets with EF and SeEF had higher glutathione reductase activity. Reduced concentrations of serum malondialdehyde were noted following Se supplementation. Diets containing Se-enriched strains showed no macroscopic effects on the liver, kidneys, heart, and brain and had no apparent influence on the basic parameters of the lipid metabolism. Both the strains tested herein showed potential for further applications as promising sources of organically bound Se and Se nanoparticles.

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