scholarly journals Mammary epithelium disruption and mammary epithelial cell exfoliation during milking in dairy cows

2017 ◽  
Vol 100 (12) ◽  
pp. 9824-9834 ◽  
Author(s):  
L. Herve ◽  
H. Quesnel ◽  
V. Lollivier ◽  
J. Portanguen ◽  
R.M. Bruckmaier ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Dairy Cows ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelium ◽  
Mammary Epithelial

scholarly journals Differential expression of living mammary epithelial cell subpopulations in milk during lactation in dairy cows

2015 ◽  
Vol 98 (10) ◽  
pp. 6897-6904 ◽  
Author(s):  
M. Baratta ◽  
M.G. Volpe ◽  
D. Nucera ◽  
G. Gabai ◽  
N. Guzzo ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Dairy Cows ◽  
Differential Expression ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Cell Subpopulations

scholarly journals Milk yield loss in response to feed restriction is associated with mammary epithelial cell exfoliation in dairy cows

2019 ◽  
Vol 102 (3) ◽  
pp. 2670-2685 ◽  
Author(s):  
L. Herve ◽  
H. Quesnel ◽  
M. Veron ◽  
J. Portanguen ◽  
J.J. Gross ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Dairy Cows ◽  
Milk Yield ◽  
Mammary Epithelial Cell ◽  
Yield Loss ◽  
Mammary Epithelial ◽  
Feed Restriction

Dynamic reciprocity revisited: a continuous, bidirectional flow of information between cells and the extracellular matrix regulates mammary epithelial cell function

1995 ◽  
Vol 73 (7-8) ◽  
pp. 391-397 ◽  
Author(s):  
C. D. Roskelley ◽  
M. J. Bissell
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Cell Function ◽  
Mammary Epithelium ◽  
Mammary Epithelial ◽  
Biochemical Signals ◽  
Bidirectional Flow ◽  
Flow Of Information

Interactions between cells and the extracellular matrix (ECM) generate two classes of signals, mechanical and biochemical. In the case of the mammary epithelial cell, both are required to initiate ECM-dependent expression of the abundant milk protein β-casein. Mechanical signals induce a cellular rounding, while functional biochemical signals are associated with an increase in tyrosine phosphorylation. These individual components are part of a complex signalling hierarchy that leads to the emergence of the fully functional lactational phenotype. Interestingly, both the assembly and disassembly of this hierarchy, which occur cyclically in vivo, are constantly modulated by dynamic and reciprocal interactions that take place within a functional unit composed of both the cell and the ECM.Key words: mammary epithelium, differentiation, extracellular matrix, casein.

scholarly journals Cell-cell interactions promote mammary epithelial cell differentiation.

1985 ◽  
Vol 100 (5) ◽  
pp. 1415-1422 ◽  
Author(s):  
J F Levine ◽  
F E Stockdale
Keyword(s):  
Monoclonal Antibodies ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Cultured Cells ◽  
Mammary Epithelium ◽  
Mammary Epithelial ◽  
3T3 Cells ◽  
Swiss 3T3 ◽  
Casein Synthesis ◽  
Cell Cell

Mammary epithelium differentiates in a stromal milieu of adipocytes and fibroblasts. To investigate cell-cell interactions that may influence mammary epithelial cell differentiation, we developed a co-culture system of murine mammary epithelium and adipocytes and other fibroblasts. Insofar as caseins are specific molecular markers of mammary epithelial differentiation, rat anti-mouse casein monoclonal antibodies were raised against the three major mouse casein components to study this interaction. Mammary epithelium from mid-pregnant mice was plated on confluent irradiated monolayers of 3T3-L1 cells, a subclone of the Swiss 3T3 cell line that differentiates into adipocytes in monolayer culture and other cell monolayers (3T3-C2 cells, Swiss 3T3 cells, and human foreskin fibroblasts). Casein was synthesized by mammary epithelium only in the presence of co-cultured cells and the lactogenic hormone combination of insulin, hydrocortisone, and prolactin. Synthesis and accumulation of alpha-, beta-, and gamma-mouse casein within the epithelium was shown by immunohistochemical staining of cultured cells with anti-casein monoclonal antibodies, and the specificity of the immunohistochemical reaction was demonstrated using immunoblots. A competitive immunoassay was used to measure the amount of casein secreted into the culture medium. In a 24-h period, mammary epithelium co-cultured with 3T3-L1 cells secreted 12-20 micrograms beta-casein per culture dish. There was evidence of specificity in the cell-cell interaction that mediates hormone-dependent casein synthesis. Swiss 3T3 cells, newborn foreskin fibroblasts, substrate-attached material ("extracellular matrix"), and tissue culture plastic did not support casein synthesis, whereas monolayers of 3T3-L1 and 3T3-C2 cells, a subclone of Swiss 3T3 cells that does not undergo adipocyte differentiation, did. We conclude that interaction between mammary epithelium and other specific nonepithelial cells markedly influences the acquisition of hormone sensitivity of the epithelium and hormone-dependent differentiation.

345 Feed restriction increases mammary epithelial cell exfoliation rate in dairy cows

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 170-171 ◽  
Author(s):  
L. Herve ◽  
M. Veron ◽  
P. Lamberton ◽  
S. Wiart ◽  
P. Debournoux ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Dairy Cows ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Feed Restriction

scholarly journals Accelerated Mammary Tumor Development in Mutant Polyomavirus Middle T Transgenic Mice Expressing Elevated Levels of Either the Shc or Grb2 Adapter Protein

1999 ◽  
Vol 19 (12) ◽  
pp. 8169-8179 ◽  
Author(s):  
Michael J. Rauh ◽  
Valerie Blackmore ◽  
Eran R. Andrechek ◽  
Christopher G. Tortorice ◽  
Roger Daly ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Mammary Tumorigenesis ◽  
Growth Factor Receptor ◽  
Mammary Epithelium ◽  
Mammary Epithelial ◽  
Adapter Proteins ◽  
Adapter Protein

ABSTRACT The Grb2 and Shc adapter proteins play critical roles in coupling activated growth factor receptors to several cellular signaling pathways. To assess the role of these molecules in mammary epithelial development and tumorigenesis, we have generated transgenic mice which individually express the Grb2 and Shc proteins in the mammary epithelium. Although mammary epithelial cell-specific expression of Grb2 or Shc accelerated ductal morphogenesis, mammary tumors were rarely observed in these strains. To explore the potential role of these adapter proteins in mammary tumorigenesis, mice coexpressing either Shc or Grb2 and a mutant form of polyomavirus middle T (PyV mT) antigen in the mammary epithelium were generated. Coexpression of either Shc or Grb2 with the mutant PyV mT antigen resulted in a dramatic acceleration of mammary tumorigenesis compared to parental mutant PyV mT strain. The increased rate of tumor formation observed in these mice was correlated with activation of the epidermal growth factor receptor family and mitogen-activated protein kinase pathway. These observations suggest that elevated levels of the Grb2 or Shc adapter protein can accelerate mammary tumor progression by sensitizing the mammary epithelial cell to growth factor receptor signaling.

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