scholarly journals Temporarily decreasing progesterone after timed artificial insemination decreased expression of interferon-tau stimulated gene 15 (ISG15) in blood leukocytes, serum pregnancy-specific protein B concentrations, and embryo size in lactating Holstein cows

2017 ◽  
Vol 100 (4) ◽  
pp. 3233-3242 ◽  
Author(s):  
P.D. Carvalho ◽  
C.C. Consentini ◽  
S.R. Weaver ◽  
R.V. Barleta ◽  
L.L. Hernandez ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Specific Protein ◽  
Holstein Cows ◽  
Interferon Tau ◽  
Blood Leukocytes ◽  
Embryo Size ◽  
Timed Artificial Insemination ◽  
Protein B

scholarly journals Characterization of Pregnancy Specific Protein B (PSPB) from Placenta Foetalis (Cotyledon) Friesian Holstein Cows

2008 ◽  
Vol 2 (1) ◽  
Author(s):  
Abdul Samik
Keyword(s):  
Western Blot ◽  
Optical Density ◽  
Early Pregnancy ◽  
Molecular Size ◽  
Specific Protein ◽  
Holstein Cows ◽  
Sds Page ◽  
Immunohistochemical Techniques ◽  
Protein B

This research was aimed to find out a substance that was useful in early pregnancy diagnosis of Friesian Holstein cows. Pregnancy Specific Protein B (PSPB) was isolated, purified and partially characterized from cotyledon Friesian Holstein cows. Characterization of PSPB protein was conducted using SDS-PAGE and Western Blot. Antisera were developed against PSPB and by immunohistochemical techniques the protein was localized to the binucleated cells of the cotyledon. The estimated molecular size of Friesian Holstein cows PSPB was 59.88 kDa with the concentration of PSPB protein in cotyledon was 6480 ng/ml. PSPB protein could induce anti-PSPB antibody with the value of optical density (OD) were 0.179±0.0102 (before immunization); 1.466±0.3288 (3rd week after immunization); 1.936±0.4754 (1st week after booster;) and 2.256±0.4842 (2nd week after booster).

scholarly journals Evaluation of synchronization protocols and methods of early pregnancy diagnosis in dairy cattle

2021 ◽  
Vol 50 (6) ◽  
Author(s):  
A.H. Shahzad ◽  
A. Sattar ◽  
N. Ahmad ◽  
I. Ahmad ◽  
M.S. Yousaf ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Early Pregnancy ◽  
Artificial Insemination ◽  
Pregnancy Loss ◽  
Holstein Cows ◽  
Plasma Progesterone ◽  
Pregnancy Diagnosis ◽  
Milk Samples ◽  
Timed Artificial Insemination ◽  
In Pregnancy

The studies aimed to evaluate the pregnancy rate (PR) for timed artificial insemination (TAI) after G7G-Ovsynch, modified G7G-Ovsynch (MG7G-Ovsynch) and Ovsynch protocols and to assess the accuracy of using pregnancy-associated glycoproteins (PAGs) and plasma progesterone (P4) in pregnancy diagnosis compared with ultrasonography (US). In study 1, Holstein cows (n = 37) were bred by TAI following the G7G-Ovsynch protocol (n = 19) or MG7G-Ovsynch (n = 18). Pregnancy was evaluated by US at days 31, 59, and 87 after breeding. The PR was not different for the G7G-Ovsynch and MG7G-Ovsynch. Blood and milk samples were collected on day 3 after insemination and then weekly through day 59 post TAI in cows diagnosed as not pregnant on day 31 and through day 87 in pregnant cows. PAGs were measured using ELISA and P4 by radioimmunoassay (RIA). In the second study, Holstein cows (n = 212) were bred by TAI following G7G-Ovsynch protocol (n = 110) or standard Ovsynch (n = 102). Cows were subjected to pregnancy diagnosis on days 30, 60, and 90. A subset (n = 15 in each group) was subjected to blood and milk samples on days 30, 45, 60, 75, and 90 to measure PAGs and P4. In study 2, PR was not significantly different between synchronization protocols on days 30, 60, and 90. Pregnancy loss averaged 15% between day 30 and day 90. The use of PAGs and P4 proved equally effective in diagnosis of pregnancy. Thus, G7G-Ovsynch was deemed the protocol of choice in postpartum cows, and PAGs assayed in milk or plasma could be used to diagnose pregnancy.

scholarly journals Effect of Synchronisation of Ovulation on Ovarian Profile and Days Open in Holstein Cows Diagnosed as Nonpregnant 26 Days after Timed Artificial Insemination

2009 ◽  
Vol 55 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Takeshi OSAWA ◽  
Sachika HONJOU ◽  
Hideki NITTA ◽  
Michiko UCHIZA ◽  
Nanae TAMEOKA ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Holstein Cows ◽  
Days Open ◽  
Timed Artificial Insemination

scholarly journals Pregnancy-Specific Protein B Induces Release of an Alpha Chemokine in Bovine Endometrium

Endocrinology ◽  
1999 ◽  
Vol 140 (1) ◽  
pp. 542-545 ◽  
Author(s):  
Kathy J. Austin ◽  
Cathy P. King ◽  
Judith E. Vierk ◽  
R. Garth Sasser ◽  
Thomas R. Hansen
Keyword(s):  
Affinity Chromatography ◽  
Primary Culture ◽  
Early Pregnancy ◽  
Specific Protein ◽  
Molar Ratio ◽  
Interferon Tau ◽  
Western Blots ◽  
Chemotactic Protein ◽  
Uterine Proteins ◽  
Protein B

Abstract Pregnancy-specific protein B (PSPB), is secreted from binucleate trophoblast of the bovine conceptus as early as day 15 of pregnancy. The objective of this experiment was to determine if PSPB induced uterine proteins. PSPB was purified from day 120 cotyledons using antibody-based affinity chromatography. Endometrium from day 14 non-pregnant cows (n = 3) was prepared for explant (3H-Leu added) culture. Radiolabeled proteins released into medium were dialyzed, separated using 1D-PAGE, and detected using fluorography and densitometry. PSPB (0, 0.5, 5, 25 & 50 nM) caused a concentration-dependent increase in the release of a radiolabeled 8-kDa uterine protein. Western blots revealed that the 8-kDa protein cross-reacted with antibody against granulocyte chemotactic protein-2 (GCP-2). PSPB also induced release of GCP-2 by bovine endometrial (BEND) cells in primary culture. The induction of GCP-2 by PSPB was blocked by addition of antiserum against PSPB (1:4 molar ratio). This is the first indication that PSPB has a hormonal role in inducing GCP-2, an alpha chemokine that also is induced by interferon-tau during early pregnancy. This chemotactic cytokine may be integral to mediating adhesion, inflammation and angiogenesis associated with early implantation.

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